| 1 | Cystic Fibrosis Foundation | Diseases, Disorders & Medical Disciplines | $1.9B | $5.0B |
| 2 | Impactassets INC | Philanthropy, Voluntarism & Grantmaking | $1.9B | $3.0B |
| 3 | Henry M Jackson Foundation for the Advancement of Military Medicine | Medical Research | $631.6M | $321.6M |
| 4 | The Keith Campbell Foundation for the Environment INC | Philanthropy, Voluntarism & Grantmaking | $377.7M | $420.2M |
| 5 | Clark Charitable Foundation INC | Philanthropy, Voluntarism & Grantmaking | $363.2M | $447.1M |
| 6 | Diana Davis Spencer Foundation | Philanthropy, Voluntarism & Grantmaking | $356.6M | $1.3B |
| 7 | Lockheed Martin Corporaton Benefit Tr for Collectively Bargained Empl | Unclassified | $236.7M | $713.8M |
| 8 | Global Communities INC | International, Foreign Affairs & National Security | $233.7M | $277.5M |
| 9 | Association of Public Health Laboratories INC | Unclassified | $142.3M | $36.1M |
| 10 | Shell Foundation | Unclassified | $127.2M | $697.1M |
| 11 | Rast Foundation | Philanthropy, Voluntarism & Grantmaking | $96.8M | $94.8M |
| 12 | National Association of Community Health Centers INC | Health Care | $86.7M | $49.4M |
| 13 | Holton-arms School INC | Education | $80.5M | $147.8M |
| 14 | J Willard and Alice S Marriott Foundation | Philanthropy, Voluntarism & Grantmaking | $79.6M | $434.5M |
| 15 | American Society of Health-system Pharmacists | Unclassified | $71.4M | $184.8M |
| 16 | American Gastroenterological Association Institute INC | Diseases, Disorders & Medical Disciplines | $70.6M | $112.3M |
| 17 | Ryuji Ueno Foundation INC | Philanthropy, Voluntarism & Grantmaking | $66.5M | $107.3M |
| 18 | National Center for Children and Families | Human Services | $66.4M | $46.8M |
| 19 | Calvert Impact Capital INC | Community Improvement & Capacity Building | $64.5M | $737.7M |
| 20 | Congressional Country Club INC | Unclassified | $62.9M | $93.3M |
| 21 | The Morningstar Philanthropic Foundation | Unclassified | $62.6M | $546.1M |
| 22 | Childrens Inn at Nih INC | Health Care | $57.6M | $93.2M |
| 23 | Hjf Medical Research International INC | Diseases, Disorders & Medical Disciplines | $57.0M | $15.3M |
| 24 | Umuc Ventures INC | Education | $50.7M | $44.5M |
| 25 | Foundation for Advanced Education in the Sciences INC | Education | $49.5M | $66.9M |
| 26 | De Beaumont Foundation INC | Philanthropy, Voluntarism & Grantmaking | $48.7M | $163.5M |
| 27 | Naomi and Nehemiah Cohen Foundation Virginia Corporation | Philanthropy, Voluntarism & Grantmaking | $48.1M | $95.6M |
| 28 | Landon School Corporation | Education | $48.0M | $143.9M |
| 29 | Educational Foundation of America | Unclassified | $47.5M | $251.2M |
| 30 | Auto Care Association | Community Improvement & Capacity Building | $42.3M | $38.1M |
| 31 | Management Leadership for Tomorrow | Education | $41.9M | $43.2M |
| 32 | Norwood School INC | Education | $37.9M | $70.5M |
| 33 | Rochambeau the French International School of Washington Dc | Unclassified | $37.6M | $121.5M |
| 34 | So What Else INC | Philanthropy, Voluntarism & Grantmaking | $34.0M | $2.5M |
| 35 | Clark-winchcole Foundation | Unclassified | $33.8M | $176.5M |
| 36 | American Association of Blood Banks | Health Care | $31.0M | $23.3M |
| 37 | American Association of Colleges of Osteopathic Medicine | Education | $31.0M | $52.5M |
| 38 | Cornell Douglas Foundation INC | Philanthropy, Voluntarism & Grantmaking | $28.6M | $57.2M |
| 39 | Kaboom INC | Recreation & Sports | $25.3M | $29.0M |
| 40 | Alley Cat Allies Incorporated | Animal-Related | $23.1M | $39.7M |
| 41 | Bethesda Country Club INC | Unclassified | $23.0M | $30.7M |
| 42 | Editorial Projects in Education INC | Arts, Culture & Humanities | $23.0M | $44.7M |
| 43 | Peter Alfond Foundation | Unclassified | $21.6M | $71.2M |
| 44 | Washington Episcopal Day School INC | Education | $20.5M | $42.9M |
| 45 | Solon E Summerfield Foundation INC | Unclassified | $20.2M | $33.8M |
| 46 | Vose River Charitable Fund | Health Care | $19.1M | $28.9M |
| 47 | Biophysical Society | Unclassified | $18.1M | $20.6M |
| 48 | Mann-paller Foundation INC | Unclassified | $17.4M | $90.9M |
| 49 | Ddw LLC | Unclassified | $16.0M | $4.8M |
| 50 | Boy Scouts of America | Unclassified | $15.3M | $44.3M |
| 51 | Hmshost Corporation Vacation Benefits Plan and Trust | Unclassified | $14.1M | $2.1M |
| 52 | Felburn Foundation | Unclassified | $14.1M | $35.5M |
| 53 | Richard E and Nancy P Marriott Foundation INC | Philanthropy, Voluntarism & Grantmaking | $13.6M | $61.6M |
| 54 | Viragh Family Foundation INC | Philanthropy, Voluntarism & Grantmaking | $12.6M | $37.1M |
| 55 | Primary Day School INC | Unclassified | $12.1M | $10.4M |
| 56 | Bridges from School to Work INC | Human Services | $12.0M | $13.3M |
| 57 | Harm Reduction Therapeutics INC | Mental Health & Crisis Intervention | $11.6M | $8.3M |
| 58 | St. Marys Housing Corporation | Unclassified | $11.6M | $61.5M |
| 59 | Car Care Council INC | Public Safety, Disaster Preparedness & Relief | $11.2M | $25.3M |
| 60 | England Family Foundation INC | Unclassified | $11.1M | $97.2M |
| 61 | Our Lady of the Woods Corporation | Unclassified | $11.0M | $19.5M |
| 62 | National Association of School Psychologists | Unclassified | $10.7M | $30.7M |
| 63 | Round House Theatre INC | Arts, Culture & Humanities | $10.7M | $12.5M |
| 64 | Burning Tree Club INC | Recreation & Sports | $10.5M | $76.4M |
| 65 | American College of Medical Genetics and Genomics | Unclassified | $10.5M | $4.4M |
| 66 | Meyer and Deanne Sharlin Foundation | Philanthropy, Voluntarism & Grantmaking | $10.4M | $8.6M |
| 67 | Washington Waldorf School INC | Education | $10.0M | $19.3M |
| 68 | Devworks International INC | Human Services | $9.4M | $1.9M |
| 69 | Deck Foundation | Philanthropy, Voluntarism & Grantmaking | $9.4M | $23.9M |
| 70 | Myles D and J Faye Sampson Family Foundation | Unclassified | $9.2M | $30.0M |
| 71 | Major League Soccer Players Association | Employment | $8.6M | $36.2M |
| 72 | The Nancy Peery Marriott Foundation INC | Philanthropy, Voluntarism & Grantmaking | $8.6M | $20.7M |
| 73 | Mater Dei School INC | Education | $8.6M | $39.5M |
| 74 | The George L Shields Foundation INC | Philanthropy, Voluntarism & Grantmaking | $8.5M | $25.0M |
| 75 | Millstream Fund INC | Unclassified | $8.4M | $16.9M |
| 76 | Calvert Impact INC | Community Improvement & Capacity Building | $8.3M | $101.8M |
| 77 | Rfi Family Foundation INC | Philanthropy, Voluntarism & Grantmaking | $7.9M | $3.5M |
| 78 | Make-a-wish Foundation of the Mid-atlantic INC | Unclassified | $7.8M | $7.3M |
| 79 | Suburban Hospital Foundation INC | Health Care | $7.6M | $34.3M |
| 80 | Putchie Foundation | Mental Health & Crisis Intervention | $7.4M | $10.6M |
| 81 | Marriott Daughters Foundation | Philanthropy, Voluntarism & Grantmaking | $7.1M | $77.9M |
| 82 | Alan and Amy Meltzer Family Foundation INC | Philanthropy, Voluntarism & Grantmaking | $7.0M | $10.4M |
| 83 | Moco Kidsco | Arts, Culture & Humanities | $6.9M | $10.1M |
| 84 | Orcid INC | Education | $6.8M | $6.9M |
| 85 | Diener School INC | Education | $6.8M | $24.4M |
| 86 | D J McManus Foundation INC | Philanthropy, Voluntarism & Grantmaking | $6.7M | $23.6M |
| 87 | Cordes Foundation | Philanthropy, Voluntarism & Grantmaking | $6.5M | $10.5M |
| 88 | Bladder Cancer Advocacy Network INC | Medical Research | $6.4M | $15.8M |
| 89 | Daba Fund | Philanthropy, Voluntarism & Grantmaking | $6.3M | $11.8M |
| 90 | National Association of Surety Bond Producers INC | Unclassified | $5.8M | $9.6M |
| 91 | Wonders Early Learning and Extended Day INC | Unclassified | $5.6M | $1.4M |
| 92 | Imagination Stage INC | Arts, Culture & Humanities | $5.4M | $5.7M |
| 93 | David and June Trone Family Foundation | Philanthropy, Voluntarism & Grantmaking | $5.3M | $5.2M |
| 94 | Curesearch for Childrens Cancer | Diseases, Disorders & Medical Disciplines | $5.2M | $7.2M |
| 95 | Pbtf Research Grove Holding Company INC | Unclassified | $5.1M | $48.8M |
| 96 | The@moses Feldman Family Foundation | Philanthropy, Voluntarism & Grantmaking | $5.1M | $59.0M |
| 97 | American Fireworks Standards Laboratory INC | Public Safety, Disaster Preparedness & Relief | $5.0M | $6.4M |
| 98 | Friends of Barker INC | Philanthropy, Voluntarism & Grantmaking | $4.7M | $17.4M |
| 99 | Stuart and Laura Plank Family Foundation INC | Philanthropy, Voluntarism & Grantmaking | $4.6M | $4.4M |
| 100 | American Gastroenterological Association INC | Community Improvement & Capacity Building | $4.5M | $5.1M |