| 1 | Howard Hughes Medical Institute Chevy Chase | H | $20.1B | $2.0B | $29.5B |
| 2 | Johns Hopkins University Baltimore | B | $20.1B | $10.5B | $23.0B |
| 3 | Kaiser Foundation Health Plan of the Mid Atlantic States INC Hyattsville | E | $6.2B | $6.0B | $3.3B |
| 4 | The Johns Hopkins Hospital Baltimore | E | $3.7B | $3.4B | $3.6B |
| 5 | Financial Industry Regulatory Authority INC Rockville | Z | $3.5B | $2.6B | $3.1B |
| 6 | A D F Community Outreach Foundation INC Mechanicsvlle | T | $3.1B | $3.1B | $37.5M |
| 7 | University of Maryland Medical System Corporation Linthicum Heights | E | $2.7B | $2.4B | $4.8B |
| 8 | Hh Medstar Health INC Columbia | E | $2.1B | $2.1B | $473.5M |
| 9 | Cystic Fibrosis Foundation Bethesda | G | $1.9B | $355.8M | $5.0B |
| 10 | Impactassets INC Bethesda | T | $1.9B | $680.3M | $3.0B |
| 11 | Washington Hospital Center Corporation Columbia | E | $1.7B | $1.7B | $793.9M |
| 12 | Medstar-georgetown Medical Center INC Columbia | E | $1.7B | $1.7B | $1.5B |
| 13 | Finra Regulation INC Rockville | Z | $1.4B | $1.4B | $94.1M |
| 14 | American Postal Workers Union Elkridge | Z | $1.3B | $1.3B | $445.7M |
| 15 | Sinai Hospital of Baltimore INC Baltimore | E | $1.2B | $1.0B | $811.2M |
| 16 | Adventist Healthcare INC Gaithersburg | Z | $1.2B | $909.8M | $1.6B |
| 17 | Tidalhealth Peninsula Regional INC Salisbury | E | $965.3M | $575.5M | $746.9M |
| 18 | Johns Hopkins All Childrens Hospital INC Baltimore | E | $939.2M | $912.8M | $1.4B |
| 19 | Healthwell Foundation Germantown | E | $935.0M | $911.5M | $918.2M |
| 20 | Neca Ibew Family Medical Care TR Fund Rockville | Y | $858.3M | $771.0M | $1.1B |
| 21 | Hood College of Frederick MD Frederick | B | $846.5M | $171.5M | $318.8M |
| 22 | Meritus Medical Center INC Hagerstown | E | $830.7M | $633.6M | $911.6M |
| 23 | Johns Hopkins Bayview Medical Center INC Baltimore | E | $807.9M | $805.6M | $459.3M |
| 24 | The Johns Hopkins Health System Corporation Baltimore | E | $714.8M | $711.5M | $2.2B |
| 25 | Holy Cross Health INC Silver Spring | Z | $714.6M | $709.0M | $1.1B |
| 26 | Jack and Nancy Dwyer Workforce Development Center INC Baltimore | E | $696.1M | $696.1M | $904.2M |
| 27 | Board of Education of Montgomery County Employee Benefit Trust Fund Rockville | Z | $690.1M | $690.1M | $19.4M |
| 28 | Luminis Health Anne Arundel Medical Center INC Annapolis | E | $686.9M | $672.1M | $1.1B |
| 29 | National Automatic Sprinkler Industry Welfare Fund Landover | Z | $673.9M | $397.5M | $787.7M |
| 30 | Seafarers Health and Benefits Plan Camp Springs | Z | $662.4M | $164.7M | $493.0M |
| 31 | Lucy Webb Haynes National Training School for Deaconesses and Mission Baltimore | E | $653.8M | $653.7M | $1.7B |
| 32 | Greater Baltimore Medical Center Baltimore | E | $638.4M | $629.2M | $901.3M |
| 33 | Mercy Medical Center INC Baltimore | E | $635.1M | $633.9M | $1.1B |
| 34 | Henry M Jackson Foundation for the Advancement of Military Medicine Bethesda | H | $631.6M | $628.9M | $321.6M |
| 35 | Franklin Square Hospital Center INC Baltimore | E | $613.1M | $613.1M | $342.0M |
| 36 | Major League Baseball Players Welfare Plan Sparks | Z | $598.1M | $103.4M | $145.9M |
| 37 | T Rowe Price Program for Charitable Giving INC Baltimore | T | $587.1M | $302.2M | $648.3M |
| 38 | United States Pharmacopeial Convention INC Rockville | E | $585.2M | $357.7M | $788.7M |
| 39 | Umsj Health System LLC Towson | E | $577.2M | $568.0M | $406.6M |
| 40 | Baltimore Washington Medical Center INC Linthicum Heights | E | $574.1M | $494.5M | $546.0M |
| 41 | Southern Maryland Electric Cooperative INC Hughesville | W | $542.8M | $542.5M | $1.2B |
| 42 | The Harry & Jeanette Weinberg Foundation INC Owings Mills | T | $539.7M | $0 | $1.8B |
| 43 | Johns Hopkins Medical Services Corporation Baltimore | E | $490.3M | $490.3M | $2.9M |
| 44 | Dimensions Health Corporation Linthicum Heights | E | $464.7M | $463.7M | $729.6M |
| 45 | Upper Chesapeake Medical Center INC Linthicum Heights | E | $460.7M | $389.1M | $849.1M |
| 46 | Frederick Health Hospital INC Frederick | E | $457.2M | $425.5M | $665.6M |
| 47 | Union Memorial Hospital Baltimore | E | $446.2M | $446.2M | $257.1M |
| 48 | National Gallery of Art Landover | A | $440.3M | $315.5M | $1.9B |
| 49 | Foundation for the National Institute of Health INC Rockville | H | $432.6M | $78.5M | $214.1M |
| 50 | State Employees Credit Union of Maryland INC Linthicum | Z | $398.8M | $333.1M | $5.5B |