| 1 | Iowa Physicians Clinic Medical Foundation | Health Care | $615.7M | $291.8M |
| 2 | Iowa Health System | Health Care | $443.7M | $1.2B |
| 3 | Lifespace Communities INC | Human Services | $393.1M | $1.3B |
| 4 | Unitypoint at Home | Unclassified | $291.5M | $95.4M |
| 5 | Care Initiatives | Health Care | $272.0M | $173.8M |
| 6 | Hy-vee Employee Benefit Plan Tr | Unclassified | $213.2M | $27.2M |
| 7 | Iowa Farm Bureau Federation | Unclassified | $131.4M | $1.8B |
| 8 | Iowa Student Loan Liquidity Corp. | Education | $115.5M | $1.6B |
| 9 | Iowa State Association of Counties | Unclassified | $57.9M | $51.0M |
| 10 | Unitypoint Rural Health Alliance Benefits Trust | Mutual & Membership Benefit | $32.7M | $12.7M |
| 11 | Barton Creek Senior Living Center INC | Housing & Shelter | $32.5M | $139.2M |
| 12 | Ifbf Property Management INC | Unclassified | $29.0M | $85.6M |
| 13 | Iec Hotel Corporation | Community Improvement & Capacity Building | $23.6M | $95.9M |
| 14 | Noble Foundation | Unclassified | $21.1M | $28.1M |
| 15 | Des Moines Golf and Country Club | Unclassified | $20.4M | $26.7M |
| 16 | Unitypoint Health at Work | Health Care | $17.5M | $6.1M |
| 17 | Link Associates | Unclassified | $16.1M | $3.5M |
| 18 | United Community Services INC | Mental Health & Crisis Intervention | $13.9M | $12.1M |
| 19 | Iowa Association for the Education of Young Children INC | Education | $11.1M | $3.5M |
| 20 | J Wesley Graham Trust Uw | Human Services | $9.3M | $13.1M |
| 21 | Deb & Bob Pulver Foundation | Philanthropy, Voluntarism & Grantmaking | $8.5M | $19.2M |
| 22 | National Forensic League | Arts, Culture & Humanities | $6.8M | $5.6M |
| 23 | Iowa Health Care Association Foundation | Unclassified | $5.3M | $321K |
| 24 | Great Outdoors Foundation | Animal-Related | $5.3M | $12.1M |
| 25 | Csa | Human Services | $5.2M | $3.0M |
| 26 | Iowa Girls High School Athletic Union | Recreation & Sports | $5.0M | $6.2M |
| 27 | Iowa Health Care Association | Unclassified | $5.0M | $3.3M |
| 28 | Chrysalis Foundation | Philanthropy, Voluntarism & Grantmaking | $4.8M | $11.4M |
| 29 | Iowa Association of Realtors | Unclassified | $4.2M | $6.2M |
| 30 | Independent Insurance Agents of Iowa | Unclassified | $3.5M | $10.0M |
| 31 | Meals from the Heartland | Food, Agriculture & Nutrition | $3.4M | $3.8M |
| 32 | Family Planning Council of Iowa | Health Care | $3.1M | $762K |
| 33 | Iowa Society of Certified Public Accountants | Unclassified | $3.1M | $3.0M |
| 34 | St. Gabriel Communications | Religion-Related | $2.7M | $5.7M |
| 35 | Schwartztrauber Good Samaritan Foundation INC | Unclassified | $2.7M | $1 |
| 36 | Deb & Jeff Hansen Foundation | Environment | $2.6M | $7.3M |
| 37 | Iowa Renewable Fuels Association | Community Improvement & Capacity Building | $2.2M | $1.8M |
| 38 | East High School Alumni Foundation | Unclassified | $2.1M | $7.1M |
| 39 | Iowa Automobile Dealers Association | Unclassified | $2.0M | $10.1M |
| 40 | Iowa Credit Union League | Unclassified | $1.9M | $87.4M |
| 41 | The Human Choice of Iowa | Human Services | $1.7M | $820K |
| 42 | Greater des Moines Montessori Association INC | Unclassified | $1.7M | $3.1M |
| 43 | Iowa Communications Alliance | Community Improvement & Capacity Building | $1.7M | $2.9M |
| 44 | West des Moines Chamber of Commerce | Community Improvement & Capacity Building | $1.6M | $1.1M |
| 45 | Iowa Optometric Association | Unclassified | $1.3M | $4.0M |
| 46 | Ames Ecumenical Housing INC | Housing & Shelter | $1.3M | $2.7M |
| 47 | Two Rivers Classical Academy | Education | $1.2M | $742K |
| 48 | Above & Beyond Cancer INC | Diseases, Disorders & Medical Disciplines | $1.2M | $861K |
| 49 | Justice League of Food | Employment | $1.2M | $660K |
| 50 | Ashworth Community Development Corporation | Community Improvement & Capacity Building | $1.1M | $198K |
| 51 | Dan and Joanie Houston Foundation | Philanthropy, Voluntarism & Grantmaking | $1.1M | $2.1M |
| 52 | Link Associates Foundation Incorporates | Unclassified | $1.1M | $5.8M |
| 53 | Plumbing Heating Cooling Contractors of Iowa | Unclassified | $1.0M | $1.5M |
| 54 | Iowa Mediation Service INC | Crime & Legal-Related | $1.0M | $1.5M |
| 55 | Beth el Jacob Synagogue Foundation | Unclassified | $1.0M | $767K |
| 56 | Iowa Association for Justice | Unclassified | $1.0M | $418K |
| 57 | Iowa Restaurant Association | Unclassified | $959K | $206K |
| 58 | Freestore | Human Services | $934K | $904K |
| 59 | Stadium Managers Association | Unclassified | $927K | $489K |
| 60 | Iowa Credit Union Foundation INC | Unclassified | $925K | $2.4M |
| 61 | Emily Anne Weikert Charitable Foundation | Unclassified | $913K | $1.5M |
| 62 | Iowa-illinois Safety Council INC | Unclassified | $911K | $926K |
| 63 | National Ttt Society | Unclassified | $891K | $1.5M |
| 64 | Iris INC | International, Foreign Affairs & National Security | $888K | $367K |
| 65 | Guideone Foundation INC | Philanthropy, Voluntarism & Grantmaking | $849K | $1.2M |
| 66 | Cedar Rapids Sheet Metal Industry Labor Management Committee | Community Improvement & Capacity Building | $780K | $2.2M |
| 67 | Sheet Metal Contractors of Iowa Industry Fund | Unclassified | $770K | $121K |
| 68 | Future Net | Education | $753K | $1.3M |
| 69 | Strands of Strength INC | Diseases, Disorders & Medical Disciplines | $752K | $2.4M |
| 70 | Keep Iowa Beautiful | Environment | $745K | $525K |
| 71 | Foundation of the Association of Former Agents of the United States | Crime & Legal-Related | $741K | $1.5M |
| 72 | West des Moines Girls Softball Association | Unclassified | $739K | $596K |
| 73 | Tai Studies Center | Unclassified | $732K | $138K |
| 74 | Central Iowa Housing Trust Fund | Community Improvement & Capacity Building | $729K | $576K |
| 75 | Iowa Heart Foundation | Medical Research | $720K | $7.5M |
| 76 | Realtor Foundation of Iowa | Unclassified | $695K | $469K |
| 77 | Professional Educators of Iowa | Unclassified | $689K | $624K |
| 78 | Chpcommunity | Health Care | $686K | $337K |
| 79 | Wolf Foundation | Philanthropy, Voluntarism & Grantmaking | $686K | $1.6M |
| 80 | Iowa Funeral Directors and Embalmers Association Incorporated | Unclassified | $674K | $4.6M |
| 81 | Story County Housing Trust | Housing & Shelter | $670K | $149K |
| 82 | West des Moines Community Schools Foundation | Philanthropy, Voluntarism & Grantmaking | $638K | $1.5M |
| 83 | McCullough Family Foundation | Philanthropy, Voluntarism & Grantmaking | $619K | $1.7M |
| 84 | Historic Valley Junction Foundation | Community Improvement & Capacity Building | $565K | $364K |
| 85 | Change Course | Employment | $557K | $63K |
| 86 | Iowa Farm Bureau Foundation | Housing & Shelter | $539K | $549K |
| 87 | Giving Back Outdoors | Animal-Related | $536K | $186K |
| 88 | Iowa Business for Clean Energy | Environment | $527K | $419K |
| 89 | Furry Friends Refuge | Animal-Related | $524K | $2.4M |
| 90 | Oskaloosa Retirement Homes INC | Unclassified | $515K | $515K |
| 91 | Iowa Council of Foundations | Education | $511K | $353K |
| 92 | Des Moines Swimming Federation | Unclassified | $510K | $103K |
| 93 | Iowa Asian Alliance Foundation | Education | $476K | $434K |
| 94 | Independent Order of Odd Fellows | Unclassified | $476K | $1.3M |
| 95 | Merchants Bonding Company Foundation | Philanthropy, Voluntarism & Grantmaking | $468K | $1.2M |
| 96 | Sheet Metal Contractors of Iowa INC | Unclassified | $456K | $245K |
| 97 | Iowa High School Speech Association | Youth Development | $456K | $360K |
| 98 | Iowa Emergencey Medical Services Association | Health Care | $448K | $502K |
| 99 | Ox Building Corporation | Public & Societal Benefit | $430K | $1.6M |
| 100 | Valley Tiger Booster Club INC | Education | $430K | $247K |