| 1 | Wells Fargo & Company Employee Benefit Trust Des Moines | Z | $2.7B | $2.7B | $14.1M |
| 2 | Prairie Meadows Race Track & Casino INC Altoona | Z | $2.5B | $2.5B | $327.6M |
| 3 | Central Iowa Hospital Corporation Des Moines | E | $2.4B | $1.4B | $1.8B |
| 4 | The Trustees of Grinnell College Grinnell | B | $1.4B | $397.4M | $3.2B |
| 5 | Catholic Health Initiatives-iowa Corp. Des Moines | E | $978.7M | $977.0M | $1.1B |
| 6 | Mercy Health Services Iowa Corp. Sioux City | E | $976.0M | $972.7M | $874.1M |
| 7 | Genesis Health System Davenport | E | $687.8M | $675.7M | $876.8M |
| 8 | Intermediaryed Mount Vernon | B | $647.7M | $352.5M | $420.5M |
| 9 | Iowa Physicians Clinic Medical Foundation Wdm | E | $615.7M | $615.1M | $291.8M |
| 10 | Mercy Medical Center Cedar Rapids | E | $606.5M | $478.8M | $728.2M |
| 11 | Veridian Credit Union Waterloo | W | $569.8M | $459.8M | $8.0B |
| 12 | St. Lukes Methodist Hospital Cedar Rapids | E | $554.2M | $502.6M | $636.9M |
| 13 | Allen Memorial Hospital Corporation Waterloo | E | $477.4M | $443.1M | $448.5M |
| 14 | Iowa Health System Wdm | E | $443.7M | $442.4M | $1.2B |
| 15 | Southeast Iowa Regional Medical Center INC W Burlington | E | $438.9M | $438.2M | $266.1M |
| 16 | Iowa State University Foundation Ames | B | $435.2M | $143.2M | $2.0B |
| 17 | State University of Iowa Foundation Iowa City | B | $435.0M | $202.5M | $2.3B |
| 18 | Covenant Medical Center INC Waterloo | E | $402.1M | $401.6M | $245.0M |
| 19 | Lifespace Communities INC Wdm | P | $393.1M | $391.2M | $1.3B |
| 20 | Drake University Des Moines | B | $363.3M | $256.3M | $628.3M |
| 21 | Community Fdn of Greater des Moines Des Moines | T | $319.8M | $160.8M | $918.7M |
| 22 | Unitypoint at Home Wdm | Z | $291.5M | $286.3M | $95.4M |
| 23 | Northwest Iowa Hospital Corporation Sioux City | E | $275.8M | $254.3M | $238.6M |
| 24 | Care Initiatives Wdm | E | $272.0M | $271.9M | $173.8M |
| 25 | Delta Dental of Iowa Johnston | Z | $270.8M | $262.0M | $180.2M |
| 26 | Trinity Regional Medical Center Fort Dodge | E | $240.9M | $206.0M | $254.4M |
| 27 | Hy-vee Employee Benefit Plan TR Wdm | Z | $213.2M | $213.2M | $27.2M |
| 28 | Jennie Edmundson Memorial Hospital Council Blfs | E | $208.1M | $172.2M | $180.9M |
| 29 | Mercy Clinics INC Des Moines | E | $203.3M | $203.3M | $275.0M |
| 30 | Iowa Bankers Benefit Plan Johnston | Z | $191.5M | $165.1M | $125.7M |
| 31 | University of Dubuque Dubuque | B | $184.2M | $104.0M | $446.9M |
| 32 | The Finley Hospital Dubuque | E | $182.0M | $160.8M | $257.3M |
| 33 | Lincoln National Corporation Voluntary Employee Bene Assoc TR Des Moines | Z | $178.0M | $178.0M | $19.6M |
| 34 | Pella Regional Health Center Pella | E | $175.6M | $159.6M | $192.4M |
| 35 | Corn Belt Power Cooperative Humboldt | Z | $171.6M | $171.6M | $570.1M |
| 36 | St. Ambrose University Davenport | B | $159.9M | $121.5M | $410.9M |
| 37 | Coe College Cedar Rapids | B | $155.6M | $81.1M | $174.7M |
| 38 | Iowa West Foundation Council Blfs | Z | $152.2M | $0 | $495.7M |
| 39 | Des Moines University Osteopathic Medical Center Des Moines | B | $151.0M | $108.2M | $522.1M |
| 40 | Wartburg College Waverly | B | $143.5M | $108.6M | $243.0M |
| 41 | Palmer College Foundation Davenport | B | $140.9M | $93.6M | $320.1M |
| 42 | University of Northern Iowa Foundation Cedar Falls | B | $140.3M | $35.6M | $290.6M |
| 43 | Mercy Hospital Iowa City | E | $138.3M | $19.7M | $44.0M |
| 44 | Luther College Decorah | B | $138.1M | $132.2M | $326.7M |
| 45 | Mercy Medical Center-clinton INC Clinton | E | $133.7M | $133.7M | $117.5M |
| 46 | Iowa Farm Bureau Federation Wdm | Z | $131.4M | $49.5M | $1.8B |
| 47 | St. Anthony Regional Hospital and Nursing Home Carroll | E | $127.5M | $113.7M | $212.9M |
| 48 | Wells Fargo & Company Short Term Disability Plan & Trust Des Moines | Y | $122.0M | $122.0M | $23.1M |
| 49 | Iowa Student Loan Liquidity Corp. Wdm | B | $115.5M | $115.4M | $1.6B |
| 50 | Northwest Iowa Power Cooperative Le Mars | Z | $114.6M | $114.6M | $279.0M |