| 1 | Iowa Soybean Association | Unclassified | $53.8M | $54.5M |
| 2 | On with Life INC | Unclassified | $19.3M | $42.5M |
| 3 | Lauridsen Family Foundation | Philanthropy, Voluntarism & Grantmaking | $13.7M | $20.8M |
| 4 | Faith Baptist Bible College | Education | $12.0M | $21.6M |
| 5 | On with Life Extended Services Ltd | Health Care | $9.8M | $4.8M |
| 6 | Ankeny Christian Academy | Unclassified | $6.5M | $7.5M |
| 7 | Donald F & Charlene K Lamberti Foundation | Philanthropy, Voluntarism & Grantmaking | $6.5M | $9.0M |
| 8 | Des Moines Area Community College Foundation | Education | $6.4M | $27.1M |
| 9 | International Brotherhood of Electrical Workers | Unclassified | $5.9M | $14.4M |
| 10 | Iowa Association of Municipal Utilities | Unclassified | $5.7M | $4.8M |
| 11 | North Central Soybean Research Program | Science & Technology | $5.6M | $2.3M |
| 12 | American Free Enterprise Chamber of Commerce | Community Improvement & Capacity Building | $5.1M | $1.0M |
| 13 | Iowa Agriculture Water Alliance | Environment | $3.9M | $2.7M |
| 14 | National Association of Sporting Goods Wholesalers | Unclassified | $3.8M | $9.0M |
| 15 | Goserv Global INC | Public Safety, Disaster Preparedness & Relief | $3.8M | $718K |
| 16 | John and Cindy Family Foundation | Philanthropy, Voluntarism & Grantmaking | $3.6M | $20.2M |
| 17 | Iowa Ffa Foundation | Unclassified | $3.6M | $14.3M |
| 18 | Iowa Concrete Paving Association | Unclassified | $2.6M | $3.4M |
| 19 | Ankeny Golf & Country Club Ankeny | Unclassified | $2.3M | $1.6M |
| 20 | Robert and Arlene Hamilton Family Charitable Foundation | Philanthropy, Voluntarism & Grantmaking | $2.2M | $2.6M |
| 21 | Ankeny Soccer Club | Recreation & Sports | $2.1M | $1.1M |
| 22 | Dcb Foundation | Philanthropy, Voluntarism & Grantmaking | $1.9M | $6.1M |
| 23 | Soil and Water Conservation Society | Environment | $1.7M | $2.0M |
| 24 | Federation of Exchange Accommodators | Unclassified | $1.4M | $4.1M |
| 25 | Hope Honoring Opportunities for Personal Empowerment | Human Services | $1.4M | $760K |
| 26 | Soybean Research Development Council | Unclassified | $1.4M | $1.2M |
| 27 | Ankeny Area Chamber of Commerce | Unclassified | $1.3M | $465K |
| 28 | Iowa Veterinary Medical Association | Unclassified | $1.2M | $2.7M |
| 29 | Future Farmers of America | Unclassified | $1.2M | $653K |
| 30 | Denny and Candy Elwell Foundation | Unclassified | $1.1M | $3.5M |
| 31 | Iowa Ready Mixed Concrete Association | Unclassified | $1.1M | $1.6M |
| 32 | National Chimney Sweep Guild | Unclassified | $1.1M | $1.1M |
| 33 | On with Life Foundation | Human Services | $960K | $2.2M |
| 34 | Cyclone Regional Training Center INC | Education | $935K | $1.5M |
| 35 | North Central Poultry Association | Food, Agriculture & Nutrition | $877K | $1.6M |
| 36 | Soy Aquaculture Alliance INC | Mutual & Membership Benefit | $875K | $758K |
| 37 | Soy Transportation Coalition | Community Improvement & Capacity Building | $861K | $1.1M |
| 38 | Agricultures Clean Water Alliance | Environment | $857K | $369K |
| 39 | Iowa Farm Bureau Federation | Unclassified | $835K | $3.4M |
| 40 | International Association of Lighting Management Companies INC | Unclassified | $727K | $653K |
| 41 | Third Party Risk Association | Community Improvement & Capacity Building | $699K | $236K |
| 42 | Missio Kaleo INC | Religion-Related | $631K | $41K |
| 43 | Murphy Charitable Family Foundation | Philanthropy, Voluntarism & Grantmaking | $627K | $4.9M |
| 44 | Agsa Corp. | Recreation & Sports | $599K | $355K |
| 45 | Hope Foundation of Kenya | International, Foreign Affairs & National Security | $583K | $204K |
| 46 | Ahepa-penelope 38 Apartments INC | Housing & Shelter | $535K | $1.6M |
| 47 | Ankeny Athletic Booster Club | Unclassified | $501K | $52K |
| 48 | Little League Baseball INC | Recreation & Sports | $440K | $462K |
| 49 | Ahepa 192-II INC | Housing & Shelter | $436K | $1.7M |
| 50 | Can Do Cancer | Health Care | $357K | $596K |
| 51 | Christian Evangelistic Mission INC | Unclassified | $349K | $93K |
| 52 | Ahepa 192-III | Housing & Shelter | $339K | $2.4M |
| 53 | Ankeny Friends of the Arts | Arts, Culture & Humanities | $325K | $264K |
| 54 | The Iowa State Poolplayers Association | Recreation & Sports | $317K | $56K |
| 55 | Ag Technology and Environmental Stewardship Foundation INC | Philanthropy, Voluntarism & Grantmaking | $317K | $196K |
| 56 | Agricultural Drainage Management Coalition INC | Environment | $299K | $29K |
| 57 | Restoring Hope International INC | International, Foreign Affairs & National Security | $299K | $280K |
| 58 | Neveln Center INC | Human Services | $283K | $33K |
| 59 | Iowa Blitz Fastpitch INC | Recreation & Sports | $281K | $34K |
| 60 | Shoulder 2 Shoulder | Food, Agriculture & Nutrition | $243K | $27K |
| 61 | Ankeny Junior Football INC | Recreation & Sports | $237K | $450K |
| 62 | Safari Club International | Unclassified | $236K | $101K |
| 63 | Miracle Travel Works INC | Health Care | $230K | $192K |
| 64 | Iowa Biodiesel Board | Community Improvement & Capacity Building | $222K | $314K |
| 65 | United Union of Roofers Waterproofers and Allied Workers | Unclassified | $216K | $196K |
| 66 | Central Iowa Coed Soccer | Recreation & Sports | $211K | $79K |
| 67 | Wiedenman Family Foundation | Human Services | $201K | $157K |
| 68 | Centennial Choral Music Parent Organization | Education | $182K | $61K |
| 69 | Amateur Athletic Union of the US INC | Recreation & Sports | $176K | $75K |
| 70 | Family Career and Community Leaders of America INC | Unclassified | $175K | $50K |
| 71 | Iowa-veterinary Medical Association Foundation | Unclassified | $172K | $449K |
| 72 | Neonatal Therapy Certification Board | Health Care | $171K | $214K |
| 73 | Karl Schooler Scholarship Tr Fund | Education | $159K | $694K |
| 74 | Iowa Foster & Adoptive Parents Assn. | Unclassified | $159K | $1.2M |
| 75 | Electrical Workers Building Foundation | Unclassified | $159K | $7.2M |
| 76 | Amadora Legacy Project INC | Health Care | $158K | $261K |
| 77 | Ankeny Basketball Club INC | Recreation & Sports | $155K | $41K |
| 78 | Foster Squad | Human Services | $152K | $70K |
| 79 | Ankeny Centennial Basketball Club | Recreation & Sports | $145K | $102K |
| 80 | Uptown Ankeny INC | Community Improvement & Capacity Building | $143K | $106K |
| 81 | Iowa Defense Counsel Association | Unclassified | $140K | $220K |
| 82 | Ankeny Business & Industry Collaborative | Community Improvement & Capacity Building | $137K | $184K |
| 83 | Ankeny Community Champions INC | Community Improvement & Capacity Building | $137K | $27K |
| 84 | Ankeny Jaguars Shooting Sports Foundation | Recreation & Sports | $130K | $46K |
| 85 | Iowa State Acacia Chapter Foundation | Education | $127K | $681K |
| 86 | Iowa Association of Agricultural Educators | Education | $126K | $178K |
| 87 | The Ankeny Klothing Exchange | Human Services | $120K | $525K |
| 88 | Light Shiners Charitable Tr | Human Services | $117K | $1.5M |
| 89 | United Union of Roofers Waterproofers & Allied Workers | Education | $113K | $139K |
| 90 | Jgr-jackson Gray Racing Corp. | Recreation & Sports | $103K | $48K |
| 91 | Iowa Ready Mixed Concrete Association Scholarship Fund | Education | $100K | $131K |
| 92 | Future Farmers of America State Associations & Local Chapter | Unclassified | $99K | $25K |
| 93 | On with Life Supportive Housing Corp. | Human Services | $97K | $664K |
| 94 | Hawks Football Committee | Recreation & Sports | $96K | $42K |
| 95 | Societa Vittoria Italiana | Unclassified | $94K | $572K |
| 96 | Autism Society of Iowa | Diseases, Disorders & Medical Disciplines | $93K | $120K |
| 97 | Ankeny Instrumental Music Foundation INC | Education | $92K | $752K |
| 98 | Ankeny Community Theatre Ltd | Arts, Culture & Humanities | $91K | $113K |
| 99 | Iowa Public Airports Association INC | Community Improvement & Capacity Building | $89K | $190K |
| 100 | Centennial Volleyball Assoc Cva | Recreation & Sports | $86K | $41K |