| 1 | Wells Fargo & Company Employee Benefit Trust Des Moines, IA | $2.7B | $14.1M |
| 2 | Prairie Meadows Race Track & Casino INC Altoona, IA | $2.5B | $327.6M |
| 3 | Central Iowa Hospital Corporation Des Moines, IA | $1.4B | $1.8B |
| 4 | Catholic Health Initiatives-iowa Corp. Des Moines, IA | $977.0M | $1.1B |
| 5 | Mercy Health Services Iowa Corp. Sioux City, IA | $972.7M | $874.1M |
| 6 | Genesis Health System Davenport, IA | $675.7M | $876.8M |
| 7 | Iowa Physicians Clinic Medical Foundation Wdm, IA | $615.1M | $291.8M |
| 8 | St. Lukes Methodist Hospital Cedar Rapids, IA | $502.6M | $636.9M |
| 9 | Mercy Medical Center Cedar Rapids, IA | $478.8M | $728.2M |
| 10 | Veridian Credit Union Waterloo, IA | $459.8M | $8.0B |
| 11 | Allen Memorial Hospital Corporation Waterloo, IA | $443.1M | $448.5M |
| 12 | Iowa Health System Wdm, IA | $442.4M | $1.2B |
| 13 | Southeast Iowa Regional Medical Center INC W Burlington, IA | $438.2M | $266.1M |
| 14 | Covenant Medical Center INC Waterloo, IA | $401.6M | $245.0M |
| 15 | The Trustees of Grinnell College Grinnell, IA | $397.4M | $3.2B |
| 16 | Lifespace Communities INC Wdm, IA | $391.2M | $1.3B |
| 17 | Intermediaryed Mount Vernon, IA | $352.5M | $420.5M |
| 18 | Unitypoint at Home Wdm, IA | $286.3M | $95.4M |
| 19 | Care Initiatives Wdm, IA | $271.9M | $173.8M |
| 20 | Delta Dental of Iowa Johnston, IA | $262.0M | $180.2M |
| 21 | Drake University Des Moines, IA | $256.3M | $628.3M |
| 22 | Northwest Iowa Hospital Corporation Sioux City, IA | $254.3M | $238.6M |
| 23 | Hy-vee Employee Benefit Plan TR Wdm, IA | $213.2M | $27.2M |
| 24 | Trinity Regional Medical Center Fort Dodge, IA | $206.0M | $254.4M |
| 25 | Mercy Clinics INC Des Moines, IA | $203.3M | $275.0M |
| 26 | State University of Iowa Foundation Iowa City, IA | $202.5M | $2.3B |
| 27 | Lincoln National Corporation Voluntary Employee Bene Assoc TR Des Moines, IA | $178.0M | $19.6M |
| 28 | Jennie Edmundson Memorial Hospital Council Blfs, IA | $172.2M | $180.9M |
| 29 | Corn Belt Power Cooperative Humboldt, IA | $171.6M | $570.1M |
| 30 | Iowa Bankers Benefit Plan Johnston, IA | $165.1M | $125.7M |
| 31 | The Finley Hospital Dubuque, IA | $160.8M | $257.3M |
| 32 | Community Fdn of Greater des Moines Des Moines, IA | $160.8M | $918.7M |
| 33 | Pella Regional Health Center Pella, IA | $159.6M | $192.4M |
| 34 | Iowa State University Foundation Ames, IA | $143.2M | $2.0B |
| 35 | Mercy Medical Center-clinton INC Clinton, IA | $133.7M | $117.5M |
| 36 | Luther College Decorah, IA | $132.2M | $326.7M |
| 37 | Wells Fargo & Company Short Term Disability Plan & Trust Des Moines, IA | $122.0M | $23.1M |
| 38 | St. Ambrose University Davenport, IA | $121.5M | $410.9M |
| 39 | Iowa Student Loan Liquidity Corp. Wdm, IA | $115.4M | $1.6B |
| 40 | Northwest Iowa Power Cooperative Le Mars, IA | $114.6M | $279.0M |
| 41 | St. Anthony Regional Hospital and Nursing Home Carroll, IA | $113.7M | $212.9M |
| 42 | Wesley Retirement Services INC Johnston, IA | $110.7M | $329.4M |
| 43 | Wartburg College Waverly, IA | $108.6M | $243.0M |
| 44 | Des Moines University Osteopathic Medical Center Des Moines, IA | $108.2M | $522.1M |
| 45 | University of Dubuque Dubuque, IA | $104.0M | $446.9M |
| 46 | Marvin M Jorgensen Charitable TR Urbandale, IA | $100.4M | $105.4M |
| 47 | Dordt University Incorporated Sioux Center, IA | $95.4M | $231.5M |
| 48 | Palmer College Foundation Davenport, IA | $93.6M | $320.1M |
| 49 | Impactlife Davenport, IA | $91.4M | $55.3M |
| 50 | Cornell College Mount Vernon, IA | $87.7M | $230.2M |