| 1 | Northwestern Memorial Healthcare Chicago, IL | $10.1B | $14.2B |
| 2 | Advocate Health and Hospitals Corporation Downers Grove, IL | $7.8B | $23.8B |
| 3 | Central States Southeast & Southwest Areas Health & Welfare F Chicago, IL | $5.5B | $16.6B |
| 4 | Feeding America Chicago, IL | $5.0B | $573.7M |
| 5 | University of Chicago Chicago, IL | $4.9B | $15.0B |
| 6 | Northwestern University Evanston, IL | $4.2B | $19.2B |
| 7 | Osf Healthcare System Peoria, IL | $3.9B | $5.8B |
| 8 | University of Chicago Medical Center Chicago, IL | $3.2B | $4.8B |
| 9 | Rush University Medical Center Chicago, IL | $3.0B | $4.7B |
| 10 | The Chicago Community Trust Chicago, IL | $2.5B | $6.0B |
| 11 | Northshore University Healthsystem Arlington Hts, IL | $2.3B | $6.8B |
| 12 | Northwestern Memorial Healthcare Chicago, IL | $2.3B | $15.2B |
| 13 | Modern Woodmen of America Rock Island, IL | $1.9B | $18.2B |
| 14 | Loyola University Medical Center Maywood, IL | $1.7B | $4.2B |
| 15 | Memorial Health System Springfield, IL | $1.6B | $2.0B |
| 16 | Ann & Robert H Lurie Childrens Hospital of Chicago Chicago, IL | $1.5B | $3.6B |
| 17 | The Carle Foundation Hospital Urbana, IL | $1.5B | $651.5M |
| 18 | Unite Here Health Oak Brook, IL | $1.5B | $1.4B |
| 19 | Advocate North Side Health Network Downers Grove, IL | $1.4B | $1.7B |
| 20 | Alliant Credit Union Chicago, IL | $1.3B | $19.5B |
| 21 | Line Construction Benefit Fund Lombard, IL | $1.2B | $1.9B |
| 22 | Loyola University of Chicago Chicago, IL | $1.1B | $2.6B |
| 23 | Delta Dental of Illinois Naperville, IL | $1.0B | $216.3M |
| 24 | Big Ten Conference INC Rosemont, IL | $928.1M | $292.3M |
| 25 | Depaul University Chicago, IL | $918.1M | $1.9B |
| 26 | Edward Hospital Arlington Hts, IL | $908.3M | $704.6M |
| 27 | Accenture United States Benefit Chicago, IL | $901.3M | $123.3M |
| 28 | Swedishamerican Hospital Rockford, IL | $888.1M | $1.2B |
| 29 | Midwest Operating Engineers Welfare Fund Countryside, IL | $801.8M | $2.3B |
| 30 | Southern Illinois Hospital Services Carbondale, IL | $765.6M | $1.5B |
| 31 | St. Vincent Hospital of the Hospital Sisters of the Third Order of St. Springfield, IL | $739.7M | $918.9M |
| 32 | Fermi Research Alliance LLC Batavia, IL | $715.5M | $18.9M |
| 33 | Blessing Hospital Quincy, IL | $704.7M | $806.6M |
| 34 | The Carle Foundation Urbana, IL | $693.3M | $3.3B |
| 35 | University of Illinois Foundation Champaign, IL | $671.3M | $3.6B |
| 36 | Elmhurst Memorial Healthcare Arlington Hts, IL | $666.6M | $695.0M |
| 37 | Endeavor Health Medical Group Arlington Hts, IL | $658.0M | $280.5M |
| 38 | Advocate Health Care Network Downers Grove, IL | $643.1M | $698.0M |
| 39 | Northwest Community Hospital Arlington Hts, IL | $633.8M | $521.7M |
| 40 | Sarah Bush Lincoln Health Center Mattoon, IL | $626.2M | $1.2B |
| 41 | Swedish Covenant Health Arlington Hts, IL | $607.5M | $336.2M |
| 42 | St. Johns Hospital of the Hospital Sisters of the Third Order of St. F Springfield, IL | $601.6M | $593.7M |
| 43 | Silver Cross Hospital & Medical Centers New Lenox, IL | $587.8M | $1.1B |
| 44 | Hospital Sisters Health System Springfield, IL | $577.8M | $582.8M |
| 45 | Adventist Midwest Health Hinsdale, IL | $574.7M | $556.2M |
| 46 | Advocate Sherman Hospital Downers Grove, IL | $561.7M | $477.7M |
| 47 | Midwest Food Bank Nfp INC Normal, IL | $556.7M | $86.6M |
| 48 | American Medical Association Chicago, IL | $546.2M | $1.5B |
| 49 | United Airlines Employee Sick Leave TR Chicago, IL | $539.7M | $61.6M |
| 50 | Midwestern University Downers Grove, IL | $537.5M | $2.1B |