| 1 | AARP Washington, DC | $11.0B | $13.1B |
| 2 | American National Red Cross Washington, DC | $3.8B | $4.6B |
| 3 | Gavi Alliance Washington, DC | $2.5B | $9.5B |
| 4 | Georgetown University Washington, DC | $2.1B | $6.0B |
| 5 | Smithsonian Institute Washington, DC | $2.0B | $7.6B |
| 6 | Childrens Hospital Washington, DC | $1.7B | $2.3B |
| 7 | Howard University Washington, DC | $1.4B | $2.7B |
| 8 | American University Washington, DC | $937.1M | $2.5B |
| 9 | American Chemical Society Washington, DC | $819.1M | $2.2B |
| 10 | Paypal Charitable Giving Fund Washington, DC | $813.4M | $106.3M |
| 11 | American Foreign Service Protective Association Washington, DC | $687.3M | $183.6M |
| 12 | New Venture Fund Washington, DC | $662.4M | $624.9M |
| 13 | Corporation for Public Broadcasting Washington, DC | $630.8M | $278.0M |
| 14 | Securities Investor Protection Corporation Washington, DC | $625.3M | $5.1B |
| 15 | Fiat Lux Risk and Insurance Company Washington, DC | $621.3M | $2.0B |
| 16 | Future Forward USA Action Washington, DC | $615.3M | $26.7M |
| 17 | Legal Services Corporation Washington, DC | $577.1M | $190.7M |
| 18 | United Mine Workers of America 1993 Benefit Plan and Trust Washington, DC | $541.6M | $216.1M |
| 19 | Pharmaceutical Research and Manufacturers of America Washington, DC | $521.3M | $349.0M |
| 20 | National Fish and Wildlife Foundation Washington, DC | $513.2M | $3.1B |
| 21 | Abax Ventures INC Washington, DC | $482.1M | $960.0M |
| 22 | The Aspen Institute INC Washington, DC | $443.8M | $756.6M |
| 23 | Pew Charitable Trusts Washington, DC | $408.9M | $1.4B |
| 24 | Catholic University of America Washington, DC | $408.7M | $897.0M |
| 25 | National Education Association of the United States Washington, DC | $402.8M | $443.0M |
| 26 | Amalgamated Charitable Foundation INC Washington, DC | $396.9M | $361.1M |
| 27 | Medical Faculty Associates INC Washington, DC | $389.5M | $264.9M |
| 28 | World Wildlife Fund INC Washington, DC | $374.8M | $783.9M |
| 29 | National Academy of Sciences Washington, DC | $361.7M | $1.6B |
| 30 | National Endowment for Democracy Washington, DC | $356.2M | $106.5M |
| 31 | Association of American Medical Colleges Washington, DC | $354.0M | $696.9M |
| 32 | Laborers-employers Benefit Plan Collection Trust Washington, DC | $348.5M | $2.1M |
| 33 | World Central Kitchen Incorporated Washington, DC | $327.8M | $221.6M |
| 34 | National Public Radio INC Washington, DC | $325.2M | $407.6M |
| 35 | Windward Fund Washington, DC | $308.2M | $432.7M |
| 36 | John F Kennedy Center for the Performing Arts Washington, DC | $306.9M | $691.4M |
| 37 | Population Services International Washington, DC | $306.3M | $257.6M |
| 38 | International City Management Association Retirement Corporation Washington, DC | $290.1M | $704.3M |
| 39 | Sixteen Thirty Fund Washington, DC | $282.2M | $106.5M |
| 40 | Service Employees International Union Washington, DC | $280.8M | $405.8M |
| 41 | National Park Foundation Washington, DC | $275.1M | $560.6M |
| 42 | World Resources Institute Washington, DC | $267.4M | $630.3M |
| 43 | United Food and Commercial Workers International Union Washington, DC | $261.8M | $677.3M |
| 44 | American Petroleum Institute Washington, DC | $252.3M | $109.9M |
| 45 | International Baccalaureate Organization Washington, DC | $250.3M | $446.0M |
| 46 | Kipp Dc Public Charter Schools Washington, DC | $243.0M | $493.8M |
| 47 | Southeastern Universities Research Association INC Washington, DC | $242.1M | $56.9M |
| 48 | Gallaudet University Washington, DC | $235.0M | $550.0M |
| 49 | Chamber of Commerce of the United States of America Washington, DC | $225.8M | $147.0M |
| 50 | Corporation for Travel Promotion Washington, DC | $224.5M | $392.6M |