| 1 | Astrazeneca Patient Assistance Organization Wilmington, DE | $4.6B | $0 |
| 2 | Christiana Care Health Services INC Wilmington, DE | $3.1B | $4.8B |
| 3 | American Online Giving Foundation INC Newark, DE | $2.1B | $269.5M |
| 4 | Bayhealth Medical Center INC Dover, DE | $1.1B | $1.8B |
| 5 | Beebe Medical Center INC Lewes, DE | $634.9M | $723.8M |
| 6 | Delaware State University Dover, DE | $266.3M | $529.9M |
| 7 | Delaware Electric Cooperative INC Greenwood, DE | $254.8M | $452.2M |
| 8 | Union Hospital of Cecil County Wilmington, DE | $194.5M | $209.2M |
| 9 | Tidalhealth Nanticoke INC Seaford, DE | $173.3M | $163.5M |
| 10 | American Gift Fund Wilmington, DE | $166.3M | $642.6M |
| 11 | St. Francis Hospital INC Wilmington, DE | $161.5M | $75.6M |
| 12 | Wilmington University INC New Castle, DE | $142.4M | $384.9M |
| 13 | The Grateful Lives Foundation Wilmington, DE | $84.8M | $1.1B |
| 14 | Food for Education Foundation INC Dover, DE | $74.9M | $93.5M |
| 15 | Five Pointe Professional Liability Insurance Company Wilmington, DE | $74.4M | $437.3M |
| 16 | Henry Francis du Pont Winterthur Museum INC Winterthur, DE | $69.3M | $439.2M |
| 17 | Conexio Care INC Claymont, DE | $63.9M | $44.6M |
| 18 | Beebe Physican Network INC Lewes, DE | $62.2M | $23.9M |
| 19 | Sunrise Project INC Wilmington, DE | $61.1M | $14.0M |
| 20 | Goodwill Industries of Delaware & Delaware County INC Wilmington, DE | $57.9M | $53.2M |
| 21 | Young Mens Christian Association of Delaware Wilmington, DE | $56.3M | $112.1M |
| 22 | Zearn Claymont, DE | $54.9M | $81.9M |
| 23 | Food Bank of Delaware INC Newark, DE | $53.4M | $60.6M |
| 24 | Sustainable Energy Utility INC Dover, DE | $48.7M | $162.6M |
| 25 | Community Systems INC New Castle, DE | $48.0M | $15.0M |
| 26 | Climate Arc INC Dover, DE | $44.4M | $30.5M |
| 27 | Unidel Foundation INC Greenville, DE | $43.6M | $484.8M |
| 28 | Odyssey Charter School Incorporated Wilmington, DE | $42.3M | $80.9M |
| 29 | Christiana Care Home Health and Community Services INC Wilmington, DE | $39.9M | $23.9M |
| 30 | Tower Hill School Wilmington, DE | $39.2M | $125.4M |
| 31 | Easter Seals Delaware & Marylands Eastern Shore INC New Castle, DE | $37.2M | $64.7M |
| 32 | Life at St. Francis Healthcare INC Wilmington, DE | $37.2M | $33.9M |
| 33 | Westside Family Healthcare INC Wilmington, DE | $34.3M | $26.2M |
| 34 | St. Andrews School of Delaware INC Middletown, DE | $34.3M | $399.3M |
| 35 | Aspira of Delaware Charter Operations Newark, DE | $32.9M | $72.1M |
| 36 | Children and Families First Delaware INC Wilmington, DE | $32.5M | $13.3M |
| 37 | Delaware Community Foundation Wilmington, DE | $32.2M | $346.1M |
| 38 | Boys and Girls Club of Delaware INC Wilmington, DE | $32.1M | $57.2M |
| 39 | Giving What We Can USA INC Newark, DE | $27.9M | $22.0M |
| 40 | Goldey Beacom College INC Wilmington, DE | $26.3M | $243.5M |
| 41 | Delaware Hospice INC Newark, DE | $25.8M | $40.8M |
| 42 | Lee INC Wilmington, DE | $24.9M | $3.6M |
| 43 | Sanford School INC Hockessin, DE | $23.0M | $35.0M |
| 44 | Mary Campbell Center Wilmington, DE | $22.9M | $28.8M |
| 45 | Mot Charter School INC Middletown, DE | $20.7M | $43.5M |
| 46 | Wilmington Country Club Wilmington, DE | $20.6M | $36.4M |
| 47 | Exceptional Care for Children INC Newark, DE | $19.8M | $47.6M |
| 48 | Lee Foundation for Civic Leadership INC Wilmington, DE | $19.5M | $6.7M |
| 49 | Milford Housing Development Corperation Milford, DE | $19.0M | $34.0M |
| 50 | Nemours New Jersey Physician Practice PC Wilmington, DE | $18.5M | $2.8M |