| 1 | Southeastern Conference Birmingham | N | $2.9B | $1.1B | $351.0M |
| 2 | University of Alabama Health Services Foundation PC Birmingham | E | $1.2B | $1.2B | $1.1B |
| 3 | Childrens Hospital of Alabama Birmingham | E | $953.2M | $953.2M | $988.2M |
| 4 | Navigate Affordable Housing Partners INC Birmingham | L | $919.3M | $919.3M | $93.2M |
| 5 | Powersouth Energy Cooperative Andalusia | Z | $859.8M | $859.7M | $2.7B |
| 6 | Corporate America Credit Union Irondale | Z | $780.4M | $230.3M | $4.6B |
| 7 | Mobile Infirmary Association Mobile | E | $723.0M | $657.9M | $881.9M |
| 8 | Auburn University Foundation Auburn | B | $435.7M | $139.2M | $1.1B |
| 9 | Gulf Health Hospitals INC Mobile | E | $429.7M | $429.6M | $411.9M |
| 10 | Noland Health Services INC Hoover | P | $420.3M | $148.5M | $573.0M |
| 11 | Samford University Birmingham | B | $400.2M | $299.7M | $905.5M |
| 12 | Haplotace Foundation Birmingham | T | $348.4M | $0 | $723.8M |
| 13 | Collegiate Housing Foundation Fairhope | L | $341.4M | $336.2M | $3.2B |
| 14 | Southern Poverty Law Center INC Montgomery | I | $339.3M | $129.1M | $822.2M |
| 15 | Jackson Hospital & Clinic INC Montgomery | E | $332.9M | $332.9M | $245.6M |
| 16 | The Health Care Authority of the City of Anniston Anniston | E | $281.0M | $281.0M | $203.7M |
| 17 | Equal Justice Initiative Montgomery | R | $276.5M | $79.6M | $455.3M |
| 18 | Corporation for the Promotion of Rifle Practice and Firearms Safety Anniston | N | $275.3M | $92.7M | $373.7M |
| 19 | The Community Foundation of Greater Birmingham Birmingham | T | $271.8M | $89.4M | $350.7M |
| 20 | Electric Membership Corp Baldwin County Electric Membership Corp. Summerdale | Z | $223.3M | $223.3M | $387.4M |
| 21 | Alabama State University Trust for Educational Excellence Montgomery | B | $222.4M | $5.9M | $113.1M |
| 22 | Joe Wheeler Electric Membership Corporation Trinity | Z | $219.2M | $219.2M | $407.1M |
| 23 | Cullman Regional Medical Center INC Cullman | E | $193.8M | $191.1M | $233.7M |
| 24 | Tuskegee University Tuskegee Institute | B | $183.1M | $183.1M | $513.8M |
| 25 | St. Vincents Birmingham Birmingham | E | $182.8M | $182.6M | $305.7M |
| 26 | Cullman Electric Cooperative Cullman | Z | $167.9M | $167.9M | $340.1M |
| 27 | Alabama Credit Union Tuscaloosa | Z | $143.3M | $143.2M | $2.0B |
| 28 | Central Alabama Electric Cooperative Prattville | Z | $134.2M | $134.2M | $466.7M |
| 29 | United Way of Central Alabama INC Birmingham | T | $133.9M | $128.2M | $141.4M |
| 30 | Max Credit Union Montgomery | Z | $123.9M | $123.8M | $2.1B |
| 31 | Southern Energy Credit Union Birmingham | Z | $119.3M | $119.3M | $3.5B |
| 32 | Civil Air Patrol Maxwell Afb | M | $109.9M | $108.6M | $247.3M |
| 33 | Infirmary Health System INC Mobile | E | $107.1M | $92.8M | $523.9M |
| 34 | Guardian Credit Union Prattville | Z | $106.7M | $93.7M | $1.1B |
| 35 | Avadian Credit Union Hoover | Z | $105.7M | $90.0M | $1.4B |
| 36 | Five Star Credit Union Dothan | Z | $93.5M | $80.1M | $1.2B |
| 37 | Listerhill Credit Union Sheffield | Z | $93.3M | $93.2M | $1.4B |
| 38 | Community Foundation of South Alabama Mobile | T | $92.7M | $15.4M | $111.4M |
| 39 | Tigers Unlimited Foundation Auburn | T | $92.1M | $62.5M | $131.2M |
| 40 | Eternal Word Television Network INC Irondale | X | $89.8M | $79.0M | $88.0M |
| 41 | Sand Mountain Electric Cooperative Rainsville | Z | $89.6M | $89.6M | $125.5M |
| 42 | Dixie Electric Cooperative Montgomery | Z | $88.3M | $88.3M | $175.4M |
| 43 | Baptist Health System INC Birmingham | E | $87.7M | $28.3M | $266.5M |
| 44 | Family Savings Credit Union Rainbow City | Z | $86.0M | $83.4M | $1.0B |
| 45 | Faulkner University Montgomery | B | $86.0M | $83.6M | $115.3M |
| 46 | St. Vincents East Birmingham | E | $84.8M | $84.3M | $125.2M |
| 47 | Tallapoosa River Electric Cooperative INC Lafayette | Z | $84.2M | $84.2M | $185.9M |
| 48 | Alabama One Credit Union Tuscaloosa | Z | $79.2M | $79.2M | $1.3B |
| 49 | Oakwood University INC Huntsville | Z | $77.1M | $71.9M | $106.7M |
| 50 | Alabama Childrens Hospital Foundation Birmingham | E | $77.0M | $76.1M | $1.6B |