| 1 | Nature Conservancy | Unclassified | $4.0B | $9.5B |
| 2 | National Rural Electric Cooperative | Unclassified | $2.3B | $862.3M |
| 3 | Railroad Employees National Health and Welfare Plan Trust | Unclassified | $1.8B | $597.2M |
| 4 | American Institutes for Research in the Behavioral Sciences | Social Science | $787.5M | $1.1B |
| 5 | National Railway Carriers & United Trans Union Health & Welfare Plan | Mutual & Membership Benefit | $644.1M | $321.0M |
| 6 | National Association of Chain Drug Stores INC | Unclassified | $616.2M | $623.2M |
| 7 | Public Broadcasting Service | Arts, Culture & Humanities | $606.5M | $654.6M |
| 8 | Stand Together Chamber of Commerce INC | Unclassified | $432.6M | $257.3M |
| 9 | Conservation International Foundation | Environment | $417.8M | $647.5M |
| 10 | Partnership for Supply Chain Management INC | Human Services | $415.7M | $135.7M |
| 11 | U S Committee for Refugees and Immigrants INC | Unclassified | $407.2M | $133.9M |
| 12 | The Conservation Fund a Nonprofit Corporation | Environment | $374.4M | $919.4M |
| 13 | Stand Together C4 Fund | Public & Societal Benefit | $257.7M | $362.9M |
| 14 | National Rural Electric Cooperative Association | Unclassified | $234.7M | $291.1M |
| 15 | United Service Organizations | Unclassified | $230.2M | $159.0M |
| 16 | Americans for Prosperity | Community Improvement & Capacity Building | $224.0M | $60.7M |
| 17 | United Service Organizations INC | Public & Societal Benefit | $186.6M | $125.0M |
| 18 | Analytic Services INC | Science & Technology | $174.0M | $265.6M |
| 19 | Stand Together Trust | Education | $172.6M | $303.8M |
| 20 | American Diabetes Association INC | Diseases, Disorders & Medical Disciplines | $165.8M | $253.9M |
| 21 | Consumer Technology Association | Community Improvement & Capacity Building | $156.9M | $433.3M |
| 22 | Cna Corporation | Unclassified | $153.6M | $160.7M |
| 23 | Greater Washington Educational Telecommunications Association INC | Arts, Culture & Humanities | $148.8M | $229.4M |
| 24 | US Civilian Research and Development Foundation | Science & Technology | $140.0M | $79.8M |
| 25 | International Justice Mission | International, Foreign Affairs & National Security | $136.1M | $97.7M |
| 26 | Marymount University | Unclassified | $126.9M | $258.0M |
| 27 | Technoserve INC | International, Foreign Affairs & National Security | $123.0M | $112.8M |
| 28 | Capital Impact Partners | Public & Societal Benefit | $122.7M | $807.0M |
| 29 | Graham Holdings Company Employee Benefits Trust | Unclassified | $114.6M | $6.3M |
| 30 | Trout Unlimited INC | Animal-Related | $114.2M | $60.7M |
| 31 | Confederation of Indian Industry | Unclassified | $110.2M | $88.0M |
| 32 | National Council on Aging INC | Unclassified | $110.2M | $23.2M |
| 33 | March of Dimes INC | Diseases, Disorders & Medical Disciplines | $103.2M | $64.2M |
| 34 | Amyotrophic Lateral Sclerosis Assn. | Diseases, Disorders & Medical Disciplines | $102.4M | $107.2M |
| 35 | American Association of Motor Vehicle Administrators | Public & Societal Benefit | $101.4M | $95.9M |
| 36 | Stand Together Foundation | Public & Societal Benefit | $101.2M | $223.4M |
| 37 | Army Emergency Relief | Public & Societal Benefit | $101.1M | $357.5M |
| 38 | Railroad Employees National Dental Plan and Trust | Unclassified | $94.7M | $47.1M |
| 39 | Ethiopian Community Development Council INC | Human Services | $92.4M | $43.9M |
| 40 | Nami National | Mental Health & Crisis Intervention | $83.1M | $77.2M |
| 41 | National Experienced Workforce Solutions INC | Employment | $81.6M | $16.0M |
| 42 | Association of State and Territorial Health Officials | Health Care | $79.7M | $18.8M |
| 43 | Cdc Small Business Finance Corp. | Community Improvement & Capacity Building | $75.0M | $133.4M |
| 44 | Communities in Schools INC | Education | $74.0M | $99.2M |
| 45 | Good Food Institute INC | Food, Agriculture & Nutrition | $71.1M | $62.0M |
| 46 | Common Application INC | Education | $70.4M | $99.2M |
| 47 | Institute for Justice | Civil Rights, Social Action & Advocacy | $68.7M | $178.0M |
| 48 | National Apartment Association | Unclassified | $65.3M | $50.2M |
| 49 | PBS Foundation | Arts, Culture & Humanities | $65.1M | $71.3M |
| 50 | American Institute in Taiwan | International, Foreign Affairs & National Security | $63.6M | $172.2M |
| 51 | Association of the United State Army INC | Public & Societal Benefit | $60.0M | $144.9M |
| 52 | Leadership Institute | Public & Societal Benefit | $59.7M | $32.2M |
| 53 | National Defense Industrial Association | International, Foreign Affairs & National Security | $59.5M | $97.5M |
| 54 | The Railrd Emplys Nati Earl Retire Major Medcl Benefit Plan Trust | Unclassified | $57.7M | $26.2M |
| 55 | Air & Space Forces Association | Education | $57.3M | $78.1M |
| 56 | Army Navy Country Club | Unclassified | $56.6M | $120.9M |
| 57 | Blumont Global Development INC | International, Foreign Affairs & National Security | $55.4M | $10.4M |
| 58 | Air Force Aid Society INC | Public & Societal Benefit | $53.9M | $244.9M |
| 59 | Navy-marine Corps Relief Society | Public & Societal Benefit | $50.2M | $148.6M |
| 60 | Robin Merger Corporation INC | Education | $49.7M | $68.0M |
| 61 | National Association of Corporate Directors | Public & Societal Benefit | $49.6M | $80.3M |
| 62 | Student Conservation Association INC | Unclassified | $48.9M | $23.4M |
| 63 | Food Marketplace INC | Unclassified | $47.9M | $86.3M |
| 64 | Susan B Anthony List INC | Education | $45.4M | $9.7M |
| 65 | Air-conditioning Heating and Refrigeration Institute | Community Improvement & Capacity Building | $45.1M | $56.0M |
| 66 | Insurance Institute for Highway Safety | Public Safety, Disaster Preparedness & Relief | $44.7M | $65.0M |
| 67 | Close Up Foundation | Arts, Culture & Humanities | $42.6M | $17.6M |
| 68 | Atlas Economic Research Foundation | Social Science | $42.3M | $22.4M |
| 69 | Ashoka | International, Foreign Affairs & National Security | $42.1M | $96.8M |
| 70 | Mercatus Center INC | Social Science | $39.4M | $36.8M |
| 71 | True Ground Housing Partners | Unclassified | $39.3M | $216.1M |
| 72 | Consumer Brands Association | Unclassified | $38.2M | $50.1M |
| 73 | National Assn of State Dept of Agriculture | Unclassified | $38.2M | $12.1M |
| 74 | Meals on Wheels America | Food, Agriculture & Nutrition | $37.8M | $44.4M |
| 75 | Rare INC | Environment | $37.6M | $35.1M |
| 76 | Casualty Actuarial Society | Unclassified | $36.9M | $33.5M |
| 77 | Ahc INC | Housing & Shelter | $36.6M | $313.7M |
| 78 | U S Dairy Export Council INC | Unclassified | $35.2M | $12.3M |
| 79 | American Public Power Assoc | Unclassified | $35.0M | $43.8M |
| 80 | State Policy Network | Civil Rights, Social Action & Advocacy | $33.9M | $24.6M |
| 81 | Infectious Diseases Society of America INC | Unclassified | $32.3M | $66.3M |
| 82 | American Society of Pension Professionals & Actuaries INC | Unclassified | $32.1M | $36.6M |
| 83 | Associated General Contractors of America | Unclassified | $32.1M | $42.9M |
| 84 | National Board for Professional Teaching Standards INC | Education | $32.0M | $16.6M |
| 85 | Aerospace Industries Association of America INC | Unclassified | $31.8M | $40.0M |
| 86 | Institute for Humane Studies | Unclassified | $31.3M | $35.5M |
| 87 | National Electrical Manufacturers Association | Unclassified | $31.2M | $19.1M |
| 88 | Child Care Aware of America | Human Services | $30.7M | $46.8M |
| 89 | Blue Investments for Nature INC | Environment | $30.7M | $466.4M |
| 90 | Bader Family Foundation | Philanthropy, Voluntarism & Grantmaking | $30.0M | $80.1M |
| 91 | American Society for Radiation Oncology | Unclassified | $28.3M | $52.5M |
| 92 | Medical Device Innovation Consortium | Medical Research | $28.2M | $11.8M |
| 93 | Coast Guard Mutual Assistance Incorporated | Public & Societal Benefit | $27.7M | $55.4M |
| 94 | Railroad Employees Health Flexible Spending Account Plan | Mutual & Membership Benefit | $27.7M | $7.8M |
| 95 | Washington Golf and Country Club INC | Unclassified | $27.3M | $52.9M |
| 96 | Spirit of America Worldwide | International, Foreign Affairs & National Security | $26.0M | $32.8M |
| 97 | Community Health Accreditation Program INC | Health Care | $25.7M | $37.5M |
| 98 | Arlington Community Foundation | Philanthropy, Voluntarism & Grantmaking | $25.4M | $35.1M |
| 99 | Global Environment & Technology Foundation | Environment | $25.0M | $19.1M |
| 100 | Croplife America | Unclassified | $23.5M | $18.3M |