| 1 | Battelle Memorial Institute | Science & Technology | $13.3B | $1.8B |
| 2 | Ohiohealth Corporation Grant Riverside Doctors Dublin Met | Health Care | $7.9B | $10.2B |
| 3 | Nationwide Childrens Hospital | Unclassified | $4.3B | $9.0B |
| 4 | Ohiohealth Corporation | Health Care | $2.3B | $1.7B |
| 5 | Jobsohio Beverage System | Community Improvement & Capacity Building | $1.8B | $1.2B |
| 6 | Nationwide Childrens Hospital INC | Health Care | $1.6B | $428.7M |
| 7 | Mount Carmel Health System | Unclassified | $1.4B | $3.2B |
| 8 | Ohio State University Physicians INC | Education | $1.2B | $528.4M |
| 9 | Columbus Foundation | Philanthropy, Voluntarism & Grantmaking | $955.8M | $2.8B |
| 10 | Buckeye Power INC | Unclassified | $915.5M | $1.6B |
| 11 | Mount Carmel Health Plan INC | Health Care | $598.5M | $501.8M |
| 12 | Nationwide Insurance Companies & Affiliates Employee Health Care Tr | Unclassified | $516.7M | $102.9M |
| 13 | Ohio State University Foundation | Education | $348.3M | $2.0B |
| 14 | Capital University | Education | $318.8M | $295.4M |
| 15 | Ohio Child Care Resource and Referral Association | Unclassified | $307.0M | $12.8M |
| 16 | Jobsohio | Employment | $301.4M | $1.4B |
| 17 | National Youth Advocate Program | Human Services | $279.0M | $67.2M |
| 18 | Nationwide Foundation | Unclassified | $250.4M | $90.2M |
| 19 | Ohio Education Assn. | Unclassified | $228.4M | $217.5M |
| 20 | Ohio Operating Engineers Health & Welfare Plan | Unclassified | $201.8M | $190.2M |
| 21 | Pediatric Academic Association INC Department of Pediatrics B-132 | Health Care | $182.3M | $20.3M |
| 22 | Franklin University | Unclassified | $178.5M | $143.9M |
| 23 | Equitas Health INC | Diseases, Disorders & Medical Disciplines | $165.9M | $53.3M |
| 24 | Battelle Voluntary Employees Beneficiary Association | Unclassified | $143.6M | $4.3M |
| 25 | National Church Residences | Housing & Shelter | $116.4M | $216.1M |
| 26 | Columbus Jewish Foundation | Philanthropy, Voluntarism & Grantmaking | $100.2M | $184.2M |
| 27 | Hospital Properties INC | Unclassified | $89.3M | $867.8M |
| 28 | 64 Telhio Credit Union INC | Unclassified | $87.9M | $1.4B |
| 29 | Ads Alliance Data Systems INC Employee Benefits Trust | Unclassified | $81.6M | $11.6M |
| 30 | Siemer Family Foundation | Philanthropy, Voluntarism & Grantmaking | $75.1M | $76.8M |
| 31 | Ohio Association of Foodbanks | Food, Agriculture & Nutrition | $73.4M | $15.6M |
| 32 | National Church Residences Foundation | Unclassified | $71.5M | $122.3M |
| 33 | Southeast INC | Mental Health & Crisis Intervention | $67.8M | $39.6M |
| 34 | Goodwill Industries of Central Ohio INC | Employment | $66.5M | $61.9M |
| 35 | Young Mens Christian Association of Central Ohio | Human Services | $66.0M | $99.2M |
| 36 | Robert F Wolfe & Edgar T Wolfe Foundation | Philanthropy, Voluntarism & Grantmaking | $65.0M | $474.7M |
| 37 | Clark County Hospital Services Corporation | Health Care | $61.3M | $42.0M |
| 38 | Ohio Dominican University | Education | $58.0M | $68.3M |
| 39 | Community Shelter Board | Unclassified | $56.4M | $27.0M |
| 40 | Foundation of the Catholic Diocese of Columbus | Religion-Related | $51.1M | $289.0M |
| 41 | Plumbers & Steamfitters 189 Welfare Fund | Unclassified | $50.4M | $63.3M |
| 42 | Pelotonia | Education | $48.7M | $32.5M |
| 43 | Alvis INC | Human Services | $45.8M | $48.3M |
| 44 | Prevailing Wage Contractors Association | Unclassified | $45.7M | $16.4M |
| 45 | Columbus College of Art & Design | Education | $45.0M | $73.0M |
| 46 | Ohio Capital Corporation for Housing | Housing & Shelter | $44.5M | $210.0M |
| 47 | Kipp Columbus | Education | $44.3M | $19.8M |
| 48 | Ohio Medical Transportation INC | Public Safety, Disaster Preparedness & Relief | $43.5M | $63.2M |
| 49 | Lifeline of Ohio Organ Procurement INC | Health Care | $42.7M | $26.2M |
| 50 | Heinzerling Community | Human Services | $41.5M | $43.5M |
| 51 | Columbus Downtown Development Corporation | Community Improvement & Capacity Building | $41.3M | $150.5M |
| 52 | Ohio Historical Society | Arts, Culture & Humanities | $40.7M | $7.5M |
| 53 | 1244 Pathways Financial Cui | Unclassified | $39.7M | $648.3M |
| 54 | Columbus Center for Human Services INC | Human Services | $39.1M | $21.5M |
| 55 | Ohio Hospital Association | Unclassified | $39.0M | $94.9M |
| 56 | Columbus Association for the Performing Arts INC | Arts, Culture & Humanities | $38.8M | $78.4M |
| 57 | The Journey Foundation | Education | $38.1M | $162.9M |
| 58 | National Registry of Emergency Medical Technicians | Public Safety, Disaster Preparedness & Relief | $37.5M | $31.5M |
| 59 | Ohio League Trust for Employee Benefits | Unclassified | $37.2M | $9.6M |
| 60 | Ohio Farm Bureau Federation INC | Unclassified | $37.1M | $55.8M |
| 61 | National Board of Boiler & Pressure Vessel Inspectors | Unclassified | $37.0M | $59.8M |
| 62 | Columbus Symphony Orchestra INC | Arts, Culture & Humanities | $36.5M | $77.4M |
| 63 | Edison Welding Institute INC | Employment | $35.2M | $25.4M |
| 64 | Maryhaven INC | Mental Health & Crisis Intervention | $34.7M | $13.7M |
| 65 | Siemer Institute | Philanthropy, Voluntarism & Grantmaking | $34.4M | $23.7M |
| 66 | Arc Industries INC of Franklin County Ohio | Unclassified | $33.3M | $74.0M |
| 67 | First Community Village | Housing & Shelter | $32.6M | $105.7M |
| 68 | Columbus Neighborhood Health Center INC | Health Care | $32.6M | $17.9M |
| 69 | Diley Ridge Medical Center | Health Care | $32.0M | $40.4M |
| 70 | Greater Columbus Arts Council INC | Arts, Culture & Humanities | $31.8M | $17.5M |
| 71 | Schottenstein-employee Benefit Plan No 552 | Unclassified | $30.9M | $2.9M |
| 72 | Legal Aid of Southeast and Central Ohio | Crime & Legal-Related | $30.8M | $26.5M |
| 73 | Cems of Ohio INC | Health Care | $30.6M | $10.0M |
| 74 | Lutheran Social Services of Central Ohio INC | Unclassified | $30.0M | $52.8M |
| 75 | Delta Gamma Fraternity Housing Corporation | Unclassified | $29.7M | $53.9M |
| 76 | Trust for the Life Ins and Hlth Pl for Collectively Brgnd Pblc Empls | Unclassified | $29.6M | $350.1M |
| 77 | Lower Lights Christian Health Center INC | Health Care | $29.5M | $16.4M |
| 78 | Trust for the City of Phoenix Arizona Post Employment Health Pla | Mutual & Membership Benefit | $28.5M | $322.4M |
| 79 | International Brotherhood of Electrical Workers | Unclassified | $28.5M | $31.1M |
| 80 | Columbus School for Girls | Education | $28.1M | $49.7M |
| 81 | Ohio High School Athletic Association | Recreation & Sports | $27.3M | $22.8M |
| 82 | Ohio Childrens Alliance | Unclassified | $27.1M | $7.6M |
| 83 | Columbus Housing Partnership INC | Housing & Shelter | $26.9M | $80.5M |
| 84 | The Methodist Retirement Center of Central Ohio | Human Services | $26.8M | $123.8M |
| 85 | Impact Community Action | Community Improvement & Capacity Building | $26.2M | $16.8M |
| 86 | Jay and Jeanie Schottenstein Foundation | Unclassified | $25.7M | $10.5M |
| 87 | Ohiohealth Corporation | Health Care | $25.6M | $55.5M |
| 88 | Franklin County Historical Society | Arts, Culture & Humanities | $25.6M | $26.8M |
| 89 | Lifecare Alliance | Food, Agriculture & Nutrition | $25.4M | $48.9M |
| 90 | United Way of Central Ohio INC | Philanthropy, Voluntarism & Grantmaking | $24.3M | $39.5M |
| 91 | Heart of Ohio Family Health Centers | Health Care | $24.3M | $17.4M |
| 92 | Strs Ohio Tx Real Estate Investments INC | Community Improvement & Capacity Building | $24.3M | $210.1M |
| 93 | Make-a-wish Foundation of Ohio Kentucky and Indiana INC | Health Care | $24.3M | $18.2M |
| 94 | Wellington School | Unclassified | $23.8M | $41.4M |
| 95 | Greater Columbus Convention & Visitors Bureau | Unclassified | $23.5M | $8.1M |
| 96 | Childrens Hunger Alliance | Food, Agriculture & Nutrition | $23.1M | $7.6M |
| 97 | Ohio Historical Society Foundation | Unclassified | $22.9M | $23.0M |
| 98 | Columbus State Community College Development Foundation INC | Philanthropy, Voluntarism & Grantmaking | $22.8M | $53.5M |
| 99 | Mount Carmel College of Nursing | Unclassified | $22.8M | $55.3M |
| 100 | Friendship Village of Columbus Ohio INC | Human Services | $22.7M | $28.8M |