| 1 | Independent Health Association INC | Health Care | $2.0B | $776.7M |
| 2 | Kaleida Health | Health Care | $2.0B | $1.3B |
| 3 | Mercy Hospital of Buffalo | Health Care | $594.3M | $288.6M |
| 4 | Sisters of Charity Hospital of Buffalo New York | Health Care | $470.9M | $291.0M |
| 5 | University at Buffalo Foundation INC | Education | $343.1M | $1.4B |
| 6 | Dyouville College | Education | $253.0M | $238.5M |
| 7 | Kenmore Mercy Hospital | Health Care | $225.4M | $165.4M |
| 8 | Catholic Health System INC | Unclassified | $210.1M | $602.2M |
| 9 | Mount St. Marys Hospital of Niagara Falls | Health Care | $160.3M | $161.9M |
| 10 | Ehs INC | Diseases, Disorders & Medical Disciplines | $124.7M | $72.7M |
| 11 | Canisius University of Buffalo New York | Education | $122.9M | $278.8M |
| 12 | U B Foundation Activities INC | Unclassified | $111.2M | $145.5M |
| 13 | Community Foundation for Greater Buffalo INC | Philanthropy, Voluntarism & Grantmaking | $87.6M | $944.0M |
| 14 | Bestself Behavioral Health INC | Unclassified | $84.9M | $110.3M |
| 15 | Buffalo Fine Arts Academy | Arts, Culture & Humanities | $81.1M | $360.9M |
| 16 | University Emergency Medical Services INC | Public Safety, Disaster Preparedness & Relief | $77.8M | $16.1M |
| 17 | Trinity Medical Wny Pc | Health Care | $67.5M | $22.4M |
| 18 | Western New York Independent Living INC | Health Care | $61.1M | $29.2M |
| 19 | Neighborhood Health Center of Wny INC | Unclassified | $59.1M | $39.6M |
| 20 | Cubrc INC | Science & Technology | $57.0M | $49.8M |
| 21 | Faculty Students Association of State University of New York | Education | $54.4M | $61.9M |
| 22 | Community Services for Every1 INC | Human Services | $54.2M | $30.0M |
| 23 | Say Yes Buffalo Scholarship INC | Education | $53.0M | $57.1M |
| 24 | Jericho Road Ministries | Religion-Related | $51.7M | $31.7M |
| 25 | University at Buffalo Neurosurgery INC | Health Care | $51.7M | $30.0M |
| 26 | McAuley-seton Home Care INC | Health Care | $49.8M | $89.5M |
| 27 | Charter School for Applied Technologies | Education | $49.0M | $65.1M |
| 28 | Trocaire College | Education | $48.1M | $51.7M |
| 29 | Sheas Oconnel Preservation Guild Ltd | Arts, Culture & Humanities | $45.2M | $29.2M |
| 30 | Buffalo City Cemetery | Mutual & Membership Benefit | $42.8M | $62.4M |
| 31 | Western New York Bloodcare INC | Health Care | $42.7M | $27.4M |
| 32 | Catholic Health System Infusion Pharmacy INC | Unclassified | $42.7M | $20.0M |
| 33 | Feedmore Western New York INC | Food, Agriculture & Nutrition | $41.0M | $9.1M |
| 34 | Roswell Park Alliance Foundation | Philanthropy, Voluntarism & Grantmaking | $40.6M | $162.8M |
| 35 | Goodwill Industries of Western Ny | Human Services | $40.0M | $33.1M |
| 36 | University of Buffalo Pediatric Associates P C | Health Care | $38.9M | $19.2M |
| 37 | Buffalo Urban Development Corporation | Community Improvement & Capacity Building | $38.3M | $171.9M |
| 38 | The Community Action Organization of Western New York INC | Unclassified | $35.9M | $17.6M |
| 39 | John R Oishei Foundation | Unclassified | $34.3M | $330.0M |
| 40 | University Psychiatric Practice INC | Mental Health & Crisis Intervention | $32.3M | $25.7M |
| 41 | University Neurology INC | Health Care | $31.9M | $8.1M |
| 42 | Young Mens Christian Association Buffalo Niagara | Human Services | $31.6M | $60.7M |
| 43 | Learning Disabilities Association of Western New York INC | Unclassified | $31.4M | $21.3M |
| 44 | The Childrens Hospital of Buffalo Foundation | Health Care | $31.1M | $84.8M |
| 45 | Catholic Health System Program of All Inclusive Care for the Elderly | Unclassified | $31.1M | $45.8M |
| 46 | Ubf Faculty-student Housing Corp. | Education | $30.5M | $147.3M |
| 47 | Catholic Charities of Buffalo | Philanthropy, Voluntarism & Grantmaking | $30.2M | $42.6M |
| 48 | Nichols School of Buffalo | Education | $29.5M | $108.7M |
| 49 | Stanley G Falk School | Unclassified | $28.8M | $7.2M |
| 50 | Mercy Flight INC | Unclassified | $28.7M | $48.6M |
| 51 | Erie County Bar Association Aid to Indigent Prisoners Society INC | Crime & Legal-Related | $28.3M | $16.7M |
| 52 | Buffalo Academy of Science Charter School | Education | $26.0M | $56.5M |
| 53 | Margaret L Wendt Foundation | Unclassified | $25.7M | $75.5M |
| 54 | Child & Family Services of Erie County | Unclassified | $25.7M | $15.3M |
| 55 | Buffalo State College Foundation INC | Education | $25.6M | $115.6M |
| 56 | Buffalo Hearing and Speech Center INC | Health Care | $24.3M | $8.4M |
| 57 | Tower Family Fund INC | Philanthropy, Voluntarism & Grantmaking | $22.0M | $40.7M |
| 58 | Health Foundation for Western & Central New York INC | Unclassified | $21.8M | $159.5M |
| 59 | Wny Catholic Long Term Care INC | Unclassified | $21.1M | $13.5M |
| 60 | Legal Aid Bureau of Buffalo INC | Crime & Legal-Related | $20.8M | $9.7M |
| 61 | Transitional Services INC | Mental Health & Crisis Intervention | $20.2M | $10.1M |
| 62 | Tapestry Charter School | Education | $19.9M | $42.1M |
| 63 | M & T Charitable Foundation | Unclassified | $19.3M | $182.2M |
| 64 | 43NORTH Foundation INC | Unclassified | $18.6M | $61.2M |
| 65 | Western New York Public Broadcasting Association | Arts, Culture & Humanities | $18.2M | $26.0M |
| 66 | Ub Family Medicine INC | Health Care | $17.3M | $6.8M |
| 67 | Girl Scouts of Western New York INC | Youth Development | $17.3M | $12.4M |
| 68 | Via Visually Impaired Advancement | Human Services | $17.0M | $21.9M |
| 69 | Cazenovia Recovery Systems INC | Unclassified | $17.0M | $27.4M |
| 70 | Community Health Center of Buffalo INC | Health Care | $16.9M | $14.8M |
| 71 | Western New York Bloodcare Foundation INC | Health Care | $16.8M | $47.7M |
| 72 | Neighborhood Legal Services INC | Unclassified | $16.6M | $8.3M |
| 73 | Villa Maria College of Buffalo | Unclassified | $16.3M | $8.0M |
| 74 | South Buffalo Charter School | Education | $16.2M | $37.8M |
| 75 | Global Concepts Charter School | Education | $15.4M | $28.5M |
| 76 | Jewish Family Services of Western New York | Human Services | $15.3M | $9.9M |
| 77 | Journeys End Refugee Services | Unclassified | $15.0M | $7.1M |
| 78 | Elmwood Village Charter School 2 | Education | $14.3M | $20.2M |
| 79 | Buffalo Philharmonic Orchestra Society INC | Arts, Culture & Humanities | $13.8M | $12.8M |
| 80 | Buffalo & Erie County Public Library | Education | $13.8M | $4.2M |
| 81 | St. Marys School for the Deaf | Unclassified | $13.6M | $10.9M |
| 82 | Buffalo Niagara Medical Campus INC | Health Care | $13.4M | $102.2M |
| 83 | Jacobs Institute INC | Medical Research | $12.5M | $7.4M |
| 84 | Reach Academy Charter School | Education | $12.1M | $23.3M |
| 85 | Ub Associates INC | Education | $11.9M | $56.2M |
| 86 | Buffalo State College Foundation Housing Corporation | Education | $11.8M | $35.7M |
| 87 | University Ophthalmology Services INC | Health Care | $11.7M | $13.0M |
| 88 | American Federation of Teachers | Unclassified | $11.5M | $7.2M |
| 89 | The Bison Childrens Scholarship Fund INC | Philanthropy, Voluntarism & Grantmaking | $11.5M | $14.6M |
| 90 | United Way of Buffalo and Erie County | Philanthropy, Voluntarism & Grantmaking | $11.0M | $28.2M |
| 91 | Hauptman-woodward Medical Research Institute INC | Medical Research | $11.0M | $27.6M |
| 92 | Buffalo Federation of Neighborhood Centers INC | Unclassified | $10.9M | $13.5M |
| 93 | Buffalo Club | Unclassified | $10.9M | $15.9M |
| 94 | Zoological Society of Buffalo INC | Animal-Related | $10.8M | $34.1M |
| 95 | Feedmore Foundation of Western New York INC | Human Services | $10.7M | $71.6M |
| 96 | University Urology INC | Education | $10.6M | $317K |
| 97 | Margaret L Wendt Foundations Holdings INC | Unclassified | $10.6M | $16K |
| 98 | Buffalo Civic Auto Ramps INC | Public & Societal Benefit | $10.4M | $3.4M |
| 99 | Buffalo Urban League INC | Human Services | $10.3M | $8.3M |
| 100 | New Era Cap Foundation | Philanthropy, Voluntarism & Grantmaking | $10.1M | $20.3M |