| 1 | Lee County Electric Cooperative INC | Unclassified | $616.9M | $1.3B |
| 2 | Echo INC | International, Foreign Affairs & National Security | $5.7M | $8.3M |
| 3 | Richard D Verleger Charitable Foundation | Philanthropy, Voluntarism & Grantmaking | $2.7M | $2.2M |
| 4 | Southwest Florida and Lee County Fair Assoc INC | Recreation & Sports | $1.6M | $1.4M |
| 5 | Lighthouse of Southwest Florida INC | Unclassified | $1.3M | $1.6M |
| 6 | Classical Christian Academy INC | Education | $1.2M | $1.1M |
| 7 | Carefree Clubhouse Corporation | Unclassified | $917K | $1.0M |
| 8 | Veterans of Foreign Wars of the United States Dept of Florida | Mutual & Membership Benefit | $449K | $351K |
| 9 | Peter S Autism School INC | Education | $368K | $377K |
| 10 | The International Order of St. Luke the Physician | Unclassified | $309K | $497K |
| 11 | Tommy Bohanon Foundation INC | Youth Development | $234K | $145K |
| 12 | Cultivate Abundance | Food, Agriculture & Nutrition | $231K | $44K |
| 13 | Living Well Mission INC | Mental Health & Crisis Intervention | $206K | $77K |
| 14 | Holiday 4 Heroes Corporation | Public & Societal Benefit | $204K | $5K |
| 15 | Inclusive Adventures INC | Human Services | $199K | $8K |
| 16 | Benevolent & Protective Order of Elks of the USA | Unclassified | $178K | $241K |
| 17 | International Brotherhood of Electrical Workers | Unclassified | $154K | $173K |
| 18 | Amvets | Unclassified | $132K | $425K |
| 19 | All Saints Home Association INC | Education | $118K | $421K |
| 20 | Boxer Rescue Angels of Florida INC | Animal-Related | $107K | $55K |
| 21 | Hubert Charitable Foundation | Philanthropy, Voluntarism & Grantmaking | $103K | $1.1M |
| 22 | Six Lakes Park Association INC | Recreation & Sports | $88K | $132K |
| 23 | Golda Circle Art House INC | Arts, Culture & Humanities | $78K | $610K |
| 24 | Rotary International | Community Improvement & Capacity Building | $61K | $52K |
| 25 | Miles of Smiles Foundation INC | Religion-Related | $58K | $334K |
| 26 | Royal Palm Ministries INC | Unclassified | $54K | $4K |
| 27 | Vietnamese Buddhist Association of Southwest Florida | Religion-Related | $53K | $191K |
| 28 | Ba Nga Afayo Initiative Uganda INC | Community Improvement & Capacity Building | $53K | $136 |
| 29 | The Uyeno Foundation INC | Education | $48K | $289K |
| 30 | North Fort Myers Academy Arts Foundation INC | Education | $47K | $93K |
| 31 | Knights of Columbus | Unclassified | $45K | $13K |
| 32 | Sabal Springs Arts and Crafts | Arts, Culture & Humanities | $43K | $5K |
| 33 | Saccavadi Dhamma Center | Religion-Related | $37K | $171K |
| 34 | Horizon Mobile Home Owners Assoc INC | Unclassified | $36K | $46K |
| 35 | Wake Up America of Lake Fairways INC | Food, Agriculture & Nutrition | $32K | $13K |
| 36 | Kids of the Future Ministry | Religion-Related | $30K | $22K |
| 37 | Packin in the Pawz Dog Rescue INC | Animal-Related | $28K | $2K |
| 38 | Keep Florida Wild Land Conservation INC | Animal-Related | $2K | $4K |
| 39 | Kaylees Angels | Mental Health & Crisis Intervention | $21 | $23 |
| 40 | Accessibility Outdoors Foundation | Recreation & Sports | $1 | $1 |
| 41 | Friend to Friend Foundation | Philanthropy, Voluntarism & Grantmaking | $1 | $694K |
| 42 | Wat Lao Dhammavanno | Religion-Related | $0 | $0 |
| 43 | Greyhound Adoption Kennel | Animal-Related | $0 | $0 |
| 44 | Herons Glen Mens Golf Association | Recreation & Sports | $0 | $0 |
| 45 | Saint Nicholas Monastery INC | Religion-Related | $0 | $0 |
| 46 | United Daughters of the Confederacy | Unclassified | $0 | $0 |
| 47 | Stan & Haney Draft Fund | Human Services | $0 | $180K |
| 48 | Ordered Steps International INC | Religion-Related | $0 | $0 |
| 49 | Iglesia Balsamo de Galaad INC | Religion-Related | $0 | $0 |
| 50 | Knights of Columbus | Unclassified | $0 | $0 |
| 51 | Toastmasters International | Unclassified | $0 | $0 |
| 52 | Herons Glen Ladies Golf Association | Recreation & Sports | $0 | $0 |
| 53 | Veterans of Foreign Wars of the United States Dept of Flordia | Unclassified | $0 | $0 |
| 54 | Cat Tails and More INC | Animal-Related | $0 | $0 |
| 55 | Sos Jesus Ministry INC | Arts, Culture & Humanities | $0 | $1 |
| 56 | Gulf Coast Worship | Mental Health & Crisis Intervention | $0 | $0 |
| 57 | Herons Glen Pickleball Association | Recreation & Sports | $0 | $0 |
| 58 | Tops Club INC | Health Care | $0 | $0 |
| 59 | Beyond Boundaries Foundation | Recreation & Sports | $0 | $0 |
| 60 | Cotree INC | Education | $0 | $0 |
| 61 | Joys Warriors | Recreation & Sports | $0 | $0 |
| 62 | Amvets | Unclassified | $0 | $0 |
| 63 | Veterans of the Glen | Public & Societal Benefit | $0 | $0 |
| 64 | Tops Club INC | Health Care | $0 | $0 |
| 65 | Butterfly Society of the Glen | Environment | $0 | $0 |
| 66 | Ghost Squadron Association | Public & Societal Benefit | $0 | $0 |
| 67 | Pray and Multiply INC | Religion-Related | $0 | $0 |
| 68 | Triumphant Community Development Co Rp | Community Improvement & Capacity Building | $0 | $0 |
| 69 | Amvets | Unclassified | $0 | $0 |
| 70 | Coastal Crushers Baseball INC | Recreation & Sports | $0 | $0 |
| 71 | Lake Fairways Community Fun | Philanthropy, Voluntarism & Grantmaking | $0 | $0 |
| 72 | Ministerio Mundial Llamados a Triunfar INC | Religion-Related | $0 | $0 |
| 73 | Guns and Hoses Pipes and Drums | Arts, Culture & Humanities | $0 | $0 |
| 74 | Swimenergy INC | Youth Development | $0 | $0 |
| 75 | Pine Lakes Fish | Human Services | $0 | $0 |
| 76 | Southwest Florida Eagle Cam Foundation INC | Animal-Related | $0 | $0 |
| 77 | Caloosa Saddle Club INC | Recreation & Sports | $0 | $0 |
| 78 | Centro Bethesda Outreach | Food, Agriculture & Nutrition | $0 | $0 |
| 79 | Parent Booster USA INC | Education | $0 | $0 |
| 80 | New Image Tabernacle Community Services Corporation | Human Services | $0 | $0 |
| 81 | Parent Booster USA INC | Education | $0 | $0 |
| 82 | Laika Raintree INC | Animal-Related | $0 | $0 |
| 83 | New Wine Worship Center | Unclassified | $0 | $0 |
| 84 | Catastrophic Health Planners INC | Health Care | $0 | $0 |
| 85 | Concerned Citizens of Bayshore Community INC | Animal-Related | $0 | $0 |
| 86 | Temple Baptist Church of Lee Co INC | Religion-Related | $0 | $0 |
| 87 | North Fort Myers First Baptist Church | Unclassified | $0 | $0 |
| 88 | Open Bible Community Church INC | Unclassified | $0 | $0 |
| 89 | Tropic Isles Baptist Church | Unclassified | $0 | $0 |
| 90 | Church of the Nazarene | Unclassified | $0 | $0 |
| 91 | Slater Road Baptist Church INC | Unclassified | $0 | $0 |
| 92 | La Leche League International INC | Unclassified | $0 | $0 |
| 93 | Holy Theotokos Monastery INC | Unclassified | $0 | $0 |
| 94 | International Church of the Golden Keys INC | Unclassified | $0 | $0 |
| 95 | Northside Baptist Church | Unclassified | $0 | $0 |
| 96 | Florida Church Planters INC | Religion-Related | $0 | $0 |
| 97 | North Shore Alliance Church | Unclassified | $0 | $0 |
| 98 | North Fort Myers Rotary Trust Fund | Unclassified | $0 | $0 |
| 99 | Foster Care Advisory Services INC | Human Services | $0 | $0 |
| 100 | Faith Assembly of God | Unclassified | $0 | $0 |