| 1 | Lakeland Regional Medical Center INC | Health Care | $1.3B | $1.2B |
| 2 | Midflorida Credit Union | Unclassified | $497.6M | $8.1B |
| 3 | Florida Southern College | Education | $271.9M | $344.3M |
| 4 | Givewell Community Foundation INC | Philanthropy, Voluntarism & Grantmaking | $196.2M | $669.9M |
| 5 | Southeastern University INC | Education | $151.7M | $141.3M |
| 6 | Lakeland Regional Health Systems INC | Health Care | $131.6M | $887.4M |
| 7 | Polk County School Readiness Coalition INC | Education | $69.2M | $7.2M |
| 8 | Publix Super Markets Charities INC | Unclassified | $62.0M | $233.8M |
| 9 | The Schools of McKeel Academy INC | Education | $36.7M | $43.0M |
| 10 | Lakeland Christian School INC | Education | $29.3M | $69.6M |
| 11 | Tri-county Human Services INC | Mental Health & Crisis Intervention | $29.0M | $24.7M |
| 12 | Carpenters Home Estates INC | Unclassified | $24.3M | $66.1M |
| 13 | Florida Methodist Foundation INC | Religion-Related | $18.6M | $246.3M |
| 14 | George Jenkins Foundation INC | Philanthropy, Voluntarism & Grantmaking | $15.6M | $11.7M |
| 15 | Bonnet Springs Park INC | Recreation & Sports | $15.0M | $154.7M |
| 16 | Votum Foundation INC | Philanthropy, Voluntarism & Grantmaking | $14.4M | $24.3M |
| 17 | Academy Prep Foundation INC | Education | $14.3M | $42.5M |
| 18 | Florida Rural Legal Services INC | Unclassified | $12.1M | $8.0M |
| 19 | Housing and Neighborhood Development Services of Central | Housing & Shelter | $11.5M | $26.3M |
| 20 | S P C a INC | Animal-Related | $10.8M | $12.9M |
| 21 | Lighthouse Ministries INC | Religion-Related | $10.5M | $9.1M |
| 22 | Sun N Fun Fly in INC | Arts, Culture & Humanities | $10.1M | $16.0M |
| 23 | New Beginnings High School | Education | $9.7M | $6.2M |
| 24 | Young Mens Christian Association of West Central Florida INC | Unclassified | $8.9M | $30.1M |
| 25 | Boys & Girls Club of Polk County | Youth Development | $8.1M | $9.2M |
| 26 | Parker Street Ministries INC | Community Improvement & Capacity Building | $7.5M | $11.5M |
| 27 | Talents 633 Ministries Nfp | Religion-Related | $5.5M | $2.9M |
| 28 | Education & Empowerment INC | Human Services | $4.9M | $641K |
| 29 | Florida Polytechnic University Foundation INC | Education | $4.5M | $12.5M |
| 30 | Achievement Academy INC | Education | $4.4M | $3.8M |
| 31 | Emgage Foundation INC | Public & Societal Benefit | $4.1M | $3.9M |
| 32 | United Food and Commercial Workers International Union | Unclassified | $4.0M | $1.2M |
| 33 | Florida Presbyterian Homes INC | Human Services | $4.0M | $58.8M |
| 34 | Lakeland Yacht and Country Club INC | Unclassified | $3.8M | $6.2M |
| 35 | Gospel INC | Human Services | $3.7M | $7.2M |
| 36 | Mi Escuela Montessori INC | Education | $3.5M | $1.5M |
| 37 | Central Florida Speech and Hearing Center | Unclassified | $3.3M | $3.5M |
| 38 | Lakeland Volunteers in Medicine INC | Health Care | $3.1M | $10.2M |
| 39 | Volunteers in Service to the Elderly INC | Human Services | $3.1M | $5.2M |
| 40 | Homeless Coalition of Polk Co INC | Unclassified | $3.1M | $3.5M |
| 41 | Geneva Classical Academy INC | Education | $3.0M | $3.8M |
| 42 | Lakeland Regional Medical Center Foundation INC | Health Care | $2.9M | $45.5M |
| 43 | Florida Childrens Museum INC | Arts, Culture & Humanities | $2.9M | $18.7M |
| 44 | Think Big for Kids INC | Youth Development | $2.7M | $3.3M |
| 45 | Tlc Petsnip INC | Animal-Related | $2.6M | $615K |
| 46 | Aerospace Center for Excellence INC | Education | $2.6M | $13.9M |
| 47 | Hugh Obrian Youth Leadership | Youth Development | $2.5M | $4.1M |
| 48 | Child Evangelism Fellowship INC | Youth Development | $2.5M | $3.3M |
| 49 | Academy Prep Center of Lakeland INC | Education | $2.4M | $708K |
| 50 | Magnolia Montessori Academy INC | Education | $2.3M | $2.9M |
| 51 | Lakeland Montessori Schoolhouse INC | Education | $2.1M | $5.8M |
| 52 | R&d Clark Family Foundation INC | Philanthropy, Voluntarism & Grantmaking | $2.1M | $1.0M |
| 53 | Dream Center of Lakeland INC | Education | $2.0M | $1.5M |
| 54 | Options for Women Pregnancy Help Clinic INC | Human Services | $2.0M | $3.5M |
| 55 | Lakeland Presbyterian Apartments INC | Housing & Shelter | $2.0M | $2.8M |
| 56 | Small Wonders Child Development Center of Lakeland INC | Human Services | $1.9M | $141K |
| 57 | Kid Pack INC | Food, Agriculture & Nutrition | $1.8M | $1.0M |
| 58 | Central Florida Development Council INC | Unclassified | $1.8M | $1.4M |
| 59 | St. Josephs Educational Foundation INC | Philanthropy, Voluntarism & Grantmaking | $1.7M | $5.7M |
| 60 | Ashley Gibson Barnett Museum of Art at Florida Southern College INC | Arts, Culture & Humanities | $1.7M | $5.5M |
| 61 | Catapult Lakeland INC | Community Improvement & Capacity Building | $1.7M | $12.1M |
| 62 | Hilda Sutton and William D Blanton Charitable Foundation INC | Unclassified | $1.6M | $3.2M |
| 63 | Top Buttons INC | Youth Development | $1.6M | $494K |
| 64 | National Watermelon Association | Unclassified | $1.5M | $1.8M |
| 65 | Lakeland Association of Realtors | Unclassified | $1.4M | $3.5M |
| 66 | Norman Family Foundation | Philanthropy, Voluntarism & Grantmaking | $1.4M | $1.8M |
| 67 | Ankit Rambabu Ministries INC | Religion-Related | $1.4M | $188K |
| 68 | Drug Prevention Resource Center INC | Unclassified | $1.4M | $1.4M |
| 69 | Campisi Family Foundation INC | Education | $1.4M | $6.8M |
| 70 | Glenn W & Hazelle Paxson Morrison Foundation INC | Philanthropy, Voluntarism & Grantmaking | $1.3M | $3.9M |
| 71 | The Shepherds Hand | Philanthropy, Voluntarism & Grantmaking | $1.3M | $10.3M |
| 72 | Lakeview Presbyterian Homes INC | Housing & Shelter | $1.3M | $2.3M |
| 73 | Florida School of Preaching | Education | $1.3M | $1.8M |
| 74 | Habitat for Humanity International INC | Unclassified | $1.3M | $3.6M |
| 75 | A Womans Choice INC a Medical Program for Women | Health Care | $1.3M | $2.2M |
| 76 | Smart Start Youth & Family Services INC | Education | $1.3M | $307K |
| 77 | Sparrow Education Foundation INC | Education | $1.2M | $71K |
| 78 | Lakeland Institute for Learning Org | Education | $1.2M | $635K |
| 79 | American Logistics Aid Network | Public Safety, Disaster Preparedness & Relief | $1.1M | $438K |
| 80 | Learning Resource Ctr of Polk Co | Education | $1.1M | $780K |
| 81 | Lakeland Area Chamber of Commerce INC | Unclassified | $1.1M | $1.1M |
| 82 | Polk Theatre INC | Arts, Culture & Humanities | $944K | $1.9M |
| 83 | Camp Fire | Unclassified | $895K | $968K |
| 84 | Swan City Soccer Club INC | Youth Development | $877K | $153K |
| 85 | Keystone Challenge Fund INC | Housing & Shelter | $869K | $814K |
| 86 | Alliance for Independence INC | Human Services | $839K | $3.2M |
| 87 | Terraviv INC | Religion-Related | $837K | $12K |
| 88 | Embrace Change Corp. | Human Services | $823K | $105K |
| 89 | Golotko Foundation INC | Philanthropy, Voluntarism & Grantmaking | $819K | $665K |
| 90 | Mies Foundation INC | Religion-Related | $801K | $169K |
| 91 | Faith Without Works INC | Human Services | $801K | $53K |
| 92 | Take Heart Project | International, Foreign Affairs & National Security | $746K | $316K |
| 93 | Florida Regional Service Office INC | Mental Health & Crisis Intervention | $746K | $290K |
| 94 | Lakeland Community Theatre INC | Arts, Culture & Humanities | $733K | $777K |
| 95 | Polk County Bully Project | Animal-Related | $724K | $54K |
| 96 | Lakeland Pigfest INC | Philanthropy, Voluntarism & Grantmaking | $724K | $187K |
| 97 | Benevolent & Protective Order of Elks of the USA | Unclassified | $723K | $621K |
| 98 | Trinity Apartments of Lakeland INC | Housing & Shelter | $718K | $4.4M |
| 99 | Veterans of Foreign Wars of the United States Dept of Florida | Unclassified | $709K | $684K |
| 100 | No Limits Sportsplex | Recreation & Sports | $703K | $750K |