| 1 | Early Learning Coalition of Floridas Gateway INC | Education | $18.5M | $2.5M |
| 2 | Belmont Academy INC | Education | $8.3M | $14.6M |
| 3 | Suwannee Valley Community Coordinated Child Care INC | Human Services | $7.0M | $3.1M |
| 4 | First Federal Foundation INC | Philanthropy, Voluntarism & Grantmaking | $5.2M | $5.5M |
| 5 | Carc-advocates for Citizens with Disabilities INC | Human Services | $4.5M | $5.6M |
| 6 | The Foundation for Florida Gateway College INC | Education | $3.4M | $29.4M |
| 7 | Lester and Anne Scaff Foundation INC | Education | $3.4M | $6.7M |
| 8 | Another Way INC | Human Services | $2.9M | $3.5M |
| 9 | United Way of Suwannee Valley INC | Unclassified | $2.8M | $2.0M |
| 10 | Lake City Christian Academy INC | Education | $2.4M | $1.4M |
| 11 | Columbia County Senior Services INC | Unclassified | $2.3M | $2.8M |
| 12 | American Legion | Unclassified | $2.1M | $435K |
| 13 | Hart 2 Hart Christian Academy INC | Education | $1.7M | $1.1M |
| 14 | Covenant Community School INC | Education | $1.4M | $833K |
| 15 | Columbia County Resources INC | Recreation & Sports | $1.3M | $2.1M |
| 16 | Happy House INC | Human Services | $1.2M | $787K |
| 17 | Cambridge Prep Academy Incorporated | Education | $1.1M | $668K |
| 18 | Lake City Columbia County Humane Society INC | Animal-Related | $999K | $1.3M |
| 19 | Joe H Anderson Jr Foundation | Philanthropy, Voluntarism & Grantmaking | $993K | $686K |
| 20 | Living Hope INC | Mental Health & Crisis Intervention | $723K | $8K |
| 21 | Stlwfar Foundation Corporation | Philanthropy, Voluntarism & Grantmaking | $720K | $5.3M |
| 22 | Pregnancy Crisis Center of Lake City INC | Unclassified | $675K | $1.4M |
| 23 | Christian Service Center of Columbia County INC | Human Services | $600K | $896K |
| 24 | Mister Mac Behavioral Consultants INC | Arts, Culture & Humanities | $593K | $165K |
| 25 | Lake City Lodge No 624 Loyal Order of Moose | Unclassified | $547K | $854K |
| 26 | Partners of Hope International | International, Foreign Affairs & National Security | $547K | $835K |
| 27 | Buck Rogers Foundation INC | Human Services | $485K | $2.7M |
| 28 | Lake City Columbia County Chamber of Commerce | Unclassified | $443K | $884K |
| 29 | Redmond Christian School | Education | $407K | $1.0M |
| 30 | Lake City Board of Realtors INC | Unclassified | $348K | $998K |
| 31 | Grace and Gratitude Sober Living INC | Human Services | $342K | $6K |
| 32 | Benevolent & Protective Order of Elks of the USA | Unclassified | $338K | $350K |
| 33 | Florida Peanut Federation INC | Community Improvement & Capacity Building | $327K | $242K |
| 34 | Richardson Community Center-annie Mattox Park North INC | Human Services | $324K | $110K |
| 35 | Florida Farm Bureau Federation INC | Unclassified | $314K | $860K |
| 36 | Caring Hearts Incorporation | Housing & Shelter | $284K | $43K |
| 37 | Veterans of Foreign Wars of the United States Dept of Florida | Unclassified | $272K | $326K |
| 38 | Lake City Columbia County Youth Baseball INC | Recreation & Sports | $256K | $87K |
| 39 | Columbia County Public Schools Foundation INC | Education | $193K | $1.5M |
| 40 | Compassion for the Family INC | International, Foreign Affairs & National Security | $168K | $26K |
| 41 | Columbia Youth Soccer Association INC | Unclassified | $161K | $96K |
| 42 | Save Our Cocker Spaniels INC | Animal-Related | $160K | $102K |
| 43 | Kingdom Gravity Ministries International INC | Education | $150K | $14K |
| 44 | Columbia County Quarterback Club INC | Recreation & Sports | $144K | $6K |
| 45 | Julie Seals Ministries INC | Religion-Related | $136K | $67K |
| 46 | Art League of North Florida INC | Arts, Culture & Humanities | $135K | $327K |
| 47 | Faith Academy INC | Education | $121K | $19K |
| 48 | Carc Housing INC | Human Services | $117K | $141K |
| 49 | Habitat for Humanity International INC | Housing & Shelter | $116K | $896K |
| 50 | Greater Lake City Community Development Corporation INC | Housing & Shelter | $100K | $989K |
| 51 | American Legion Auxiliary | Unclassified | $97K | $0 |
| 52 | Yesahcan Sanctuary INC | Animal-Related | $92K | $1 |
| 53 | Florida Association Medical Staff Services INC | Health Care | $86K | $70K |
| 54 | Estate Planning Council of Northeast Florida | Mutual & Membership Benefit | $84K | $46K |
| 55 | Rotary Club of Lake City | Unclassified | $77K | $94K |
| 56 | Ames Christian University INC | Education | $73K | $13K |
| 57 | International Brotherhood of Electrical Workers | Unclassified | $73K | $23K |
| 58 | Kiwanis International INC | Unclassified | $71K | $41K |
| 59 | Shiftforward INC | Human Services | $69K | $210K |
| 60 | Lexis Kitty Corner Incorporated | Animal-Related | $60K | $1 |
| 61 | Blue Gray Army INC | Arts, Culture & Humanities | $45K | $80K |
| 62 | Mustard Tree Ministries INC | Religion-Related | $37K | $6K |
| 63 | B&s Combs Elks Lodge 1599 INC | Mutual & Membership Benefit | $31K | $143K |
| 64 | Community Concerts of Lake City INC | Unclassified | $28K | $64K |
| 65 | Lake City Citizens Police Academy Alumni Association INC | Education | $21K | $36K |
| 66 | Ember Global Vision INC | Human Services | $18K | $13K |
| 67 | Destined for a Purpose INC | Housing & Shelter | $14K | $13K |
| 68 | Mtm Capital Holdings INC | Community Improvement & Capacity Building | $10K | $600K |
| 69 | Columbia County Adult Softball | Unclassified | $4K | $17K |
| 70 | Florida Alpha Educational Foundation INC | Unclassified | $164 | $678K |
| 71 | Career Connections 386 INC | Employment | $1 | $1 |
| 72 | Visionary Veterans | Public & Societal Benefit | $1 | $1 |
| 73 | Grace Harbor Ministries INC | Religion-Related | $0 | $0 |
| 74 | Women of God Ministries in Christ INC | Religion-Related | $0 | $0 |
| 75 | Fellowship Church of Lake City | Religion-Related | $0 | $0 |
| 76 | Spirit of Christ Lutheran Church | Unclassified | $0 | $0 |
| 77 | Catholic Charities Bureau INC Lake City Regional Office | Unclassified | $0 | $0 |
| 78 | Columbia County Riding Club at Winfield INC | Recreation & Sports | $0 | $0 |
| 79 | Alliance of Divine Love INC | Unclassified | $0 | $0 |
| 80 | Alliance of Divine Love INC | Unclassified | $0 | $0 |
| 81 | New Generation Educational Services | Education | $0 | $0 |
| 82 | Healing Hands International Ministries INC | Religion-Related | $0 | $0 |
| 83 | North Florida Center of Excellence INC | Education | $0 | $0 |
| 84 | Ramona Park Church INC | Unclassified | $0 | $0 |
| 85 | Order of the Eastern Star of Florida | Unclassified | $0 | $0 |
| 86 | Free & Accepted Masons of Florida | Unclassified | $0 | $0 |
| 87 | Free & Accepted Masons of Florida | Unclassified | $0 | $0 |
| 88 | Rotary International | Unclassified | $0 | $0 |
| 89 | Crime Stoppers of Columbia Countyinc | Crime & Legal-Related | $0 | $0 |
| 90 | The Orchard Community Church | Unclassified | $0 | $0 |
| 91 | W2 Ministries Incorporated | Religion-Related | $0 | $0 |
| 92 | Florida Cattlemens County Association INC | Food, Agriculture & Nutrition | $0 | $0 |
| 93 | Iglesia Evangelica Aposento Alto INC | Unclassified | $0 | $0 |
| 94 | Heroes in the Real World INC | Arts, Culture & Humanities | $0 | $0 |
| 95 | Muna International INC | International, Foreign Affairs & National Security | $0 | $0 |
| 96 | The Ichetucknee Partnership INC | Environment | $0 | $0 |
| 97 | Pride of B and S Combs Elks Temple | Mutual & Membership Benefit | $0 | $0 |
| 98 | Call to Life Ministries | Unclassified | $0 | $0 |
| 99 | Fishin for Heroes | Public & Societal Benefit | $0 | $0 |
| 100 | Lake City Honor Guard INC | Arts, Culture & Humanities | $0 | $0 |