| 1 | Christian & Missionary Alliance Foundation INC | Human Services | $194.7M | $749.6M |
| 2 | Gmp-employers Retiree Trust | Unclassified | $118.4M | $597.6M |
| 3 | Harry Chapin Food Bank of Southwest Florida INC | Food, Agriculture & Nutrition | $104.6M | $52.0M |
| 4 | The Early Learning Coalition of Southwest Florida INC | Education | $74.0M | $8.3M |
| 5 | Goodwill Industries of Southwest Florida INC | Employment | $63.1M | $108.7M |
| 6 | Cypress Cove at Healthpark Florida INC | Human Services | $62.4M | $298.1M |
| 7 | Florida Gulf Coast University Foundation INC | Education | $61.0M | $230.1M |
| 8 | Love a Child INC | International, Foreign Affairs & National Security | $44.5M | $42.6M |
| 9 | Southwest Florida Community Foundation INC | Philanthropy, Voluntarism & Grantmaking | $42.5M | $183.5M |
| 10 | Area Agency on Aging for Southwest Florida INC | Unclassified | $32.0M | $13.2M |
| 11 | Saluscare INC | Unclassified | $31.7M | $28.2M |
| 12 | Habitat for Humanity of Lee & Hendry Counties INC | Housing & Shelter | $30.7M | $44.7M |
| 13 | Canterbury School Corporation | Education | $29.3M | $32.8M |
| 14 | United Way of Lee County INC | Philanthropy, Voluntarism & Grantmaking | $27.7M | $14.9M |
| 15 | Evangelical Christian School INC | Education | $23.9M | $55.2M |
| 16 | McGregor Clinic INC | Health Care | $21.5M | $5.8M |
| 17 | Florida Gulf Coast University Financing Corporation | Philanthropy, Voluntarism & Grantmaking | $20.7M | $175.7M |
| 18 | Daniel R & Anne M Harper Foundation INC | Philanthropy, Voluntarism & Grantmaking | $18.7M | $12.4M |
| 19 | Florida Southwestern State College Foundation INC | Education | $14.9M | $57.2M |
| 20 | Health Planning Council of Sw Fla | Health Care | $14.0M | $3.8M |
| 21 | Southwest Florida Workforce Development Board INC | Employment | $13.5M | $3.9M |
| 22 | Lee Memorial Hospital INC | Unclassified | $10.1M | $9.0M |
| 23 | Florida Southwestern State College Financing Corporation | Education | $9.9M | $35.3M |
| 24 | Almira Family Foundation | Philanthropy, Voluntarism & Grantmaking | $7.9M | $16.6M |
| 25 | Gulf Coast Humane Society INC | Animal-Related | $7.8M | $14.6M |
| 26 | Healthy Start Coalition of Southwest Florida INC | Recreation & Sports | $7.6M | $6.3M |
| 27 | Rubenstein Family Foundation INC | Philanthropy, Voluntarism & Grantmaking | $7.1M | $6.7M |
| 28 | Shalimare Incorporated | Human Services | $6.6M | $2.5M |
| 29 | The Edward and Gale McBride Foundation INC | Unclassified | $6.2M | $18.3M |
| 30 | Bernard E & Edith B Waterman Charitable Foundation | Unclassified | $6.1M | $28.5M |
| 31 | Baba Foundation INC | Philanthropy, Voluntarism & Grantmaking | $6.0M | $6.0M |
| 32 | Abuse Counseling and Treatment INC | Mental Health & Crisis Intervention | $6.0M | $6.2M |
| 33 | Florida Cancer Specialists Foundation INC | Philanthropy, Voluntarism & Grantmaking | $6.0M | $10.4M |
| 34 | Alliance of Casa la Linda Corporation | Housing & Shelter | $5.7M | $9.4M |
| 35 | Community Cooperative INC | Human Services | $5.6M | $6.2M |
| 36 | Thomas Edison and Henry Ford Winter Estates INC | Arts, Culture & Humanities | $5.5M | $4.9M |
| 37 | Vetjobs | Employment | $5.5M | $10.3M |
| 38 | Childrens Advocacy Center of Sw Florida INC | Human Services | $5.2M | $5.8M |
| 39 | Carol B Phelon Foundation | Philanthropy, Voluntarism & Grantmaking | $5.0M | $17.0M |
| 40 | Royal Palm Coast Realtor Association INC | Unclassified | $4.8M | $17.0M |
| 41 | Southwest Florida Affordablehousing Choice Foundation INC | Housing & Shelter | $4.6M | $20.4M |
| 42 | Center for Progress and Excellence INC | Mental Health & Crisis Intervention | $4.6M | $905K |
| 43 | City of Palms Charter High School INC | Education | $4.6M | $3.4M |
| 44 | Lee County Housing Development Corporation | Housing & Shelter | $4.4M | $1.9M |
| 45 | The Legacy Foundation at Shell Point INC | Philanthropy, Voluntarism & Grantmaking | $4.3M | $3.9M |
| 46 | The Swarm Collective INC | Philanthropy, Voluntarism & Grantmaking | $4.2M | $1.3M |
| 47 | John E & Aliese Price Foundation INC | Unclassified | $4.0M | $9.9M |
| 48 | Larc INC | Employment | $4.0M | $6.9M |
| 49 | Sterling Center | Mental Health & Crisis Intervention | $3.9M | $1.4M |
| 50 | If I Can Dream Foundation | Employment | $3.7M | $956K |
| 51 | Valeries House INC | Human Services | $3.5M | $6.9M |
| 52 | Crestwell Higher Learning School INC | Education | $3.2M | $4.1M |
| 53 | Captains for Clean Water INC | Environment | $3.2M | $4.0M |
| 54 | Heights Center INC | Community Improvement & Capacity Building | $3.2M | $509K |
| 55 | Swfl Childrens Charities INC | Philanthropy, Voluntarism & Grantmaking | $3.1M | $1.3M |
| 56 | Quality Life Center of Southwest Florida INC | Community Improvement & Capacity Building | $3.1M | $5.1M |
| 57 | St. Charles Yacht Club INC | Unclassified | $3.0M | $7.2M |
| 58 | Premier Mobile Health Services Corporation | Unclassified | $2.8M | $665K |
| 59 | Fred & Jean Allegretti Foundation INC | Philanthropy, Voluntarism & Grantmaking | $2.8M | $15.2M |
| 60 | Bobby Nichols-fiddlesticks Foundation INC | Philanthropy, Voluntarism & Grantmaking | $2.7M | $516K |
| 61 | Ronald McDonald House Charities of Southwest Florida INC | Health Care | $2.6M | $8.1M |
| 62 | Interfaith Charities of South Lee INC | Human Services | $2.6M | $870K |
| 63 | Boy Scouts of America | Unclassified | $2.5M | $13.1M |
| 64 | Dix Family Foundation | Unclassified | $2.5M | $577K |
| 65 | De Lasalle Academy of Fort Myers INC | Education | $2.4M | $4.4M |
| 66 | Heights Charter School INC | Education | $2.4M | $556K |
| 67 | Gladiolus Food Pantry INC | Food, Agriculture & Nutrition | $2.3M | $2.1M |
| 68 | Boys & Girls Clubs of Lee County INC | Youth Development | $2.3M | $3.3M |
| 69 | Lee County Legal Aid Society | Unclassified | $2.2M | $2.7M |
| 70 | Gulf Coast Symphony Orchestra INC | Arts, Culture & Humanities | $2.2M | $34K |
| 71 | Military Spouse Corporate Career Network INC | Human Services | $2.2M | $1.4M |
| 72 | The Foundation for Lee County Public Schools INC | Unclassified | $2.2M | $6.6M |
| 73 | Charity Pros INC | Human Services | $2.0M | $2.4M |
| 74 | Dunbar Improvement Association INC | Unclassified | $1.9M | $6.9M |
| 75 | Shady Rest Foundation INC | Health Care | $1.8M | $2.5M |
| 76 | Colonnade Foundation | Philanthropy, Voluntarism & Grantmaking | $1.8M | $3.0M |
| 77 | Our Mothers Home of Southwest Florida INC | Human Services | $1.8M | $2.3M |
| 78 | Fort Myers Presbyterian Community INC | Housing & Shelter | $1.7M | $2.9M |
| 79 | Florida Arts INC | Arts, Culture & Humanities | $1.6M | $5.0M |
| 80 | Animal Refuge Center INC | Animal-Related | $1.6M | $4.3M |
| 81 | Imaginarium Group INC | Arts, Culture & Humanities | $1.6M | $513K |
| 82 | Independent Development Services Corporation | Unclassified | $1.6M | $364K |
| 83 | Hawkins Family Foundation INC | Philanthropy, Voluntarism & Grantmaking | $1.6M | $1.5M |
| 84 | Hearts & Homes for Veterans INC | Public & Societal Benefit | $1.5M | $1.4M |
| 85 | The Cape Coral Kiwanis Foundation INC | Philanthropy, Voluntarism & Grantmaking | $1.5M | $4.4M |
| 86 | Dr Piper Center for Social Services INC | Human Services | $1.5M | $819K |
| 87 | Southwest Florida Symphony Orchestra and Chorus Association I | Arts, Culture & Humanities | $1.4M | $3.0M |
| 88 | Guardians of Florida Animal Rescue INC | Animal-Related | $1.4M | $713K |
| 89 | Southwest Florida Safety Council INC | Unclassified | $1.4M | $845K |
| 90 | Sadako K Judd Private Foundation INC | Philanthropy, Voluntarism & Grantmaking | $1.3M | $1.9M |
| 91 | Affordable Homeownership Foundation INC | Crime & Legal-Related | $1.3M | $3.2M |
| 92 | Crosspointe Academy INC | Education | $1.3M | $513K |
| 93 | Tice & the Shores Lodge No 1297 Loyal Order of Moose | Unclassified | $1.2M | $1.6M |
| 94 | Panira Healthcare Clinic INC | Health Care | $1.2M | $305K |
| 95 | Park Family Foundation | Philanthropy, Voluntarism & Grantmaking | $1.2M | $5.1M |
| 96 | Protected Harbor INC | Mental Health & Crisis Intervention | $1.2M | $2.1M |
| 97 | Source of Light and Hope Development Center INC | Human Services | $1.1M | $317K |
| 98 | International Association of Fire Fighters | Unclassified | $1.1M | $900K |
| 99 | Haven of Hope International INC | International, Foreign Affairs & National Security | $1.1M | $551K |
| 100 | Junior Achievement of Southwest Florida | International, Foreign Affairs & National Security | $1.1M | $1.0M |