| 1 | The Mitre Corporation Bedford | $2.5B | $2.5B | $1.2B |
| 2 | Charles Stark Draper Laboratory INC Cambridge | $944.5M | $871.1M | $817.0M |
| 3 | Woods Hole Oceanographic Institution Woods Hole | $393.9M | $344.3M | $785.1M |
| 4 | Convergent Research INC Cambridge | $72.4M | $72.4M | $108.0M |
| 5 | Marine Biological Laboratory Woods Hole | $59.5M | $57.3M | $245.0M |
| 6 | Woodwell Climate Research Center Falmouth | $32.8M | $32.6M | $66.4M |
| 7 | Lab Central INC Cambridge | $27.3M | $27.3M | $110.7M |
| 8 | American Meteorological Society Boston | $21.1M | $18.4M | $28.5M |
| 9 | Terc INC Cambridge | $19.7M | $14.4M | $20.9M |
| 10 | Sia Foundation INC Randolph | $12.4M | $12.3M | $42.6M |
| 11 | Probabilistic Computing Foundation INC Cambridge | $11.8M | $0 | $24.9M |
| 12 | Science for America INC Cambridge | $10.9M | $10.9M | $21.3M |
| 13 | Gloucester Marine Genomics Institute Incorporated Gloucester | $9.9M | $9.9M | $13.2M |
| 14 | World Wide Web Consortium INC Wakefield | $8.7M | $8.7M | $7.0M |
| 15 | Securebio INC Cambridge | $7.3M | $7.3M | $8.5M |
| 16 | American Association of Variable Star Observers Becket | $7.2M | $1.1M | $19.1M |
| 17 | Igem Foundation INC Cambridge | $6.5M | $6.5M | $1.7M |
| 18 | Fab Foundation Boston | $6.5M | $6.5M | $4.4M |
| 19 | Tricontinental Ltd Northhampton | $6.3M | $858K | $9.7M |
| 20 | Stockholm Environment Institute Usinc Somerville | $6.3M | $5.6M | $4.8M |
| 21 | Massachusetts Biomedical Initiatives Worcester | $6.2M | $5.3M | $31.3M |
| 22 | MIT Real Estate Foundation INC Cambridge | $6.1M | $4.3M | $4.8M |
| 23 | Earthwatch Institute INC Newton | $5.5M | $3.9M | $5.2M |
| 24 | Science and Technology Futures INC Cambridge | $5.1M | $5.1M | $10.2M |
| 25 | Massautonomy INC N Falmouth | $4.9M | $4.9M | $2.0M |
| 26 | Consortium of Universities for the Advancement of Hydrologic Science Arlington | $4.8M | $4.8M | $2.5M |
| 27 | Cortico Corporation Boston | $3.6M | $3.6M | $6.6M |
| 28 | James M and Cathleen D Stone Family Foundation INC Boston | $3.6M | $0 | $2.3M |
| 29 | Boston Biostatistics Research Fdn INC Framingham | $3.5M | $3.5M | $14.9M |
| 30 | Seeding Labs a Non Profit Corporation Boston | $3.5M | $3.5M | $5.5M |
| 31 | Microscopy Society of America Wakefield | $2.9M | $2.8M | $5.3M |
| 32 | Northeast Radio Observatory Corporation Cambridge | $2.7M | $2.7M | $579K |
| 33 | Research Institute for Learning and Development Lexington | $2.5M | $2.5M | $1.5M |
| 34 | Nucleate Bio INC Brighton | $2.4M | $2.4M | $1.0M |
| 35 | Association for the Advancement of Sustainability in Higher Education Boston | $2.4M | $2.3M | $1.3M |
| 36 | Aging Biology Foundation INC Waltham | $2.3M | $0 | $26K |
| 37 | Atlantic White Shark Conservancy INC North Chatham | $2.1M | $1.9M | $2.7M |
| 38 | International Society of Protistologists INC Woods Hole | $2.0M | $191K | $1.7M |
| 39 | Prpl Foundation INC Wakefield | $1.8M | $1.8M | $4.4M |
| 40 | Engineering Center Education Trust Boston | $1.7M | $1.4M | $3.0M |
| 41 | Openreview Foundation Newton Highlands | $1.6M | $1.6M | $2.8M |
| 42 | Tatta Bio Cambridge | $1.6M | $1.6M | $1.9M |
| 43 | Mansfield Bio-incubator INC Mansfield | $1.6M | $1.6M | $7.1M |
| 44 | Participatory Culture Foundation Boston | $1.4M | $1.4M | $887K |
| 45 | American Society of Civil Engineers Boston | $1.4M | $615K | $2.7M |
| 46 | Bioinnovations Institute Natick | $1.3M | $1.3M | $74K |
| 47 | T1d Exchange INC Boston | $1.3M | $336K | $7.4M |
| 48 | Human Cell Atlas INC Cambridge | $1.2M | $1.2M | $2.4M |
| 49 | Corundum Convergence Institute Cambridge | $998K | $998K | $739K |
| 50 | American Association for Aerosol Research INC Wakefield | $830K | $830K | $2.7M |