| 1 | Dana-farber Cancer Institute Boston | $3.5B | $3.5B | $5.3B |
| 2 | Broad Institute INC Cambridge | $845.6M | $831.1M | $2.9B |
| 3 | Whitehead Institute for Biomedical Research Cambridge | $209.9M | $164.9M | $903.4M |
| 4 | Dana Farber Mass General Brigham Cancer Care INC Boston | $137.6M | $137.6M | $25.8M |
| 5 | Institute for Protein Innovation INC Boston | $74.2M | $7.5M | $204.5M |
| 6 | Alzheimers Disease Research Foundation Wellesley Hls | $37.8M | $31.8M | $28.6M |
| 7 | Boston Va Research Institute INC Boston | $29.6M | $12.6M | $29.2M |
| 8 | Addgene INC Watertown | $26.4M | $26.4M | $56.7M |
| 9 | Zoll Foundation INC Chelmsford | $26.3M | $0 | $27.4M |
| 10 | Baim Institute for Clinical Research INC Boston | $19.0M | $19.0M | $30.2M |
| 11 | Reliant Foundation INC Worcester | $15.2M | $2.5M | $29.0M |
| 12 | Advanced Medical Research Foundation INC Chestnut Hill | $14.7M | $6.6M | $82.6M |
| 13 | Als Therapy Development Foundation INC Watertown | $14.6M | $13.4M | $23.1M |
| 14 | South Shore Health Express INC S Weymouth | $14.0M | $14.0M | $14.2M |
| 15 | Health Effects Institute Boston | $13.0M | $13.0M | $14.0M |
| 16 | Center for Information and Study on Clinical Research Participation Boston | $12.9M | $12.7M | $8.4M |
| 17 | Life Science Cares INC Cambridge | $12.9M | $12.5M | $12.0M |
| 18 | International Myeloma Society Boston | $11.8M | $11.8M | $10.8M |
| 19 | Count Me in INC Cambridge | $11.8M | $11.8M | $22.4M |
| 20 | The Progeria Research Foundation INC Peabody | $10.5M | $3.7M | $48.0M |
| 21 | National Scleroderma Foundation Danvers | $8.8M | $6.6M | $11.3M |
| 22 | Vivli INC Burlington | $6.9M | $6.9M | $10.6M |
| 23 | Foundation for Neurologic Diseases Newburyport | $6.4M | $4.4M | $49.2M |
| 24 | Myasthenia Gravis Foundation of America INC Westborough | $6.3M | $5.7M | $13.6M |
| 25 | Jett Foundation INC Norwell | $6.2M | $5.1M | $6.5M |
| 26 | Digital Medicine Society INC Boston | $5.6M | $5.6M | $4.9M |
| 27 | Openbiome Foundation INC Westport | $5.5M | $2.2M | $8.9M |
| 28 | The Ricbac Foundation Newtonville | $5.3M | $0 | $44.2M |
| 29 | Fshd Society Randolph | $5.0M | $4.9M | $5.6M |
| 30 | Silent Spring Institute INC Newton | $4.7M | $4.6M | $5.7M |
| 31 | Haymakers for Hope INC Wellesley | $4.6M | $4.6M | $548K |
| 32 | Center for Human Genetics INC Cambridge | $4.4M | $4.4M | $13.6M |
| 33 | Giovanni Armenise-harvard Foundation for Scientific Research Boston | $3.6M | $3.6M | $85.5M |
| 34 | Termeer Institute INC Marblehead | $3.5M | $3.5M | $2.1M |
| 35 | Amyloidosis Research Consortium INC Newton | $3.4M | $3.4M | $4.7M |
| 36 | Detectogether INC Westborough | $3.2M | $2.5M | $5.4M |
| 37 | Fraxa Research Foundation INC Newburyport | $2.4M | $2.3M | $5.0M |
| 38 | Alzforum Foundation INC Boston | $2.4M | $0 | $3.3M |
| 39 | Intrepid Alliance INC Cambridge | $2.2M | $2.2M | $2.3M |
| 40 | Phare Bio INC Boston | $2.1M | $2.1M | $2.6M |
| 41 | Cancollaborate Bedford | $2.1M | $2.1M | $1.8M |
| 42 | Northeast Als Consortium INC Boston | $2.1M | $2.1M | $3.1M |
| 43 | Beat Childhood Cancer Foundation INC Braintree | $2.1M | $1.6M | $2.8M |
| 44 | Lown Institute INC Needham | $2.0M | $2.0M | $4.7M |
| 45 | Albert F Egan Jr & Dorothy H Egan Foundation INC Nantucket | $1.9M | $0 | $17.3M |
| 46 | Punch 4 Parkinsons Corporation Walpole | $1.9M | $1.9M | $174K |
| 47 | Ocular Immunology and Uveitis Foundation INC Weston | $1.9M | $1.6M | $3.0M |
| 48 | Collaborative Trajectory Analysis Project Cambridge | $1.9M | $1.9M | $952K |
| 49 | The Kittredge Foundation Boston | $1.8M | $0 | $3.3M |
| 50 | Sumaira Foundation Somerville | $1.5M | $1.3M | $619K |