| 1 | Conservation International Foundation Arlington | $417.8M | $271.2M | $647.5M |
| 2 | The Conservation Fund a Nonprofit Corporation Arlington | $374.4M | $373.2M | $919.4M |
| 3 | Mattress Recycling Council Alexandria | $55.3M | $41.8M | $28.9M |
| 4 | Rare INC Arlington | $37.6M | $33.6M | $35.1M |
| 5 | Water Environment Federation Alexandria | $33.4M | $31.9M | $46.4M |
| 6 | Blue Investments for Nature INC Arlington | $30.7M | $23.7M | $466.4M |
| 7 | Volgenau Foundation McLean | $29.4M | $0 | $166.2M |
| 8 | National Recreation & Park Association Ashburn | $26.4M | $24.7M | $26.9M |
| 9 | Global Environment & Technology Foundation Arlington | $25.0M | $19.5M | $19.1M |
| 10 | American Horticultural Society INC Alexandria | $17.5M | $10.1M | $13.7M |
| 11 | Norfolk Botanical Garden INC Norfolk | $17.3M | $16.2M | $38.8M |
| 12 | Ward Burton Wild Life Foundation Halifax | $16.6M | $14.7M | $37.9M |
| 13 | Virginia Environmental Endowment Richmond | $15.7M | $3.8M | $32.4M |
| 14 | American Energy Action Alexandria | $15.4M | $15.4M | $11.4M |
| 15 | Global Water Challenge Arlington | $14.3M | $14.3M | $20.0M |
| 16 | Lewis Ginter Botanical Garden INC Richmond | $14.1M | $13.5M | $42.9M |
| 17 | Piedmont Environmental Council Warrenton | $13.0M | $12.0M | $11.6M |
| 18 | Council on Library and Information Resources Alexandria | $11.3M | $11.3M | $20.8M |
| 19 | Green Blue Institute Charlottesvle | $10.7M | $10.7M | $8.6M |
| 20 | Natureserve Arlington | $10.5M | $9.7M | $8.0M |
| 21 | Capital Region Land Conservancy INC Richmond | $9.6M | $5.7M | $13.3M |
| 22 | Land Trust of Virginia INC Middleburg | $8.9M | $1.9M | $8.7M |
| 23 | Health Care Without Harm Reston | $8.5M | $8.5M | $18.8M |
| 24 | Norfolk Botanical Gardens Foundation Norfolk | $7.4M | $232K | $15.6M |
| 25 | James River Association Richmond | $6.7M | $6.3M | $13.9M |
| 26 | Local Energy Alliance Program INC Charlottesvle | $6.1M | $6.1M | $1.0M |
| 27 | The Lamb Center Fairfax | $5.6M | $5.5M | $15.6M |
| 28 | Fonds de Preservation de la Biodiversite Au Gabon INC Arlington | $5.5M | $5.5M | $6.2M |
| 29 | Energy Systems Integration Group INC Reston | $5.4M | $5.4M | $4.4M |
| 30 | Public Democracy America Great Falls | $5.2M | $5.2M | $364K |
| 31 | Ecological Trust Fund of Panama Arlington | $4.8M | $3.8M | $44.6M |
| 32 | Global Forest Generation INC Arlington | $4.6M | $4.6M | $4.1M |
| 33 | Garden Club of Virginia INC Richmond | $4.6M | $1.9M | $13.4M |
| 34 | Co2 Coalition INC Fairfax | $4.5M | $4.5M | $3.5M |
| 35 | Generation180 Charlottesvle | $3.8M | $3.8M | $2.4M |
| 36 | Beneficial Electrification League Arlington | $3.8M | $3.8M | $1.5M |
| 37 | Cave Conservancy of the Virginias Glen Allen | $3.7M | $613K | $2.3M |
| 38 | Sustainable Conservation INC Arlington | $3.7M | $3.7M | $31.4M |
| 39 | Natures Capital INC Arlington | $3.6M | $3.6M | $2.2M |
| 40 | Nature for Justice Falls Church | $3.5M | $3.5M | $2.1M |
| 41 | Elizabeth River Project Norfolk | $3.4M | $3.4M | $13.9M |
| 42 | Resource Protection Group INC Gainesville | $3.2M | $258K | $5.6M |
| 43 | Association of State Drinking Water Administrators Arlington | $2.7M | $1.8M | $4.6M |
| 44 | Conservation Allies Warrenton | $2.6M | $2.6M | $974K |
| 45 | Friends of the Rappahannock INC Fredericksbrg | $2.5M | $2.4M | $3.1M |
| 46 | The Piedmont Foundation Warrenton | $2.4M | $2.4M | $27.5M |
| 47 | Willowsford Conservancy Aldie | $2.4M | $2.2M | $8.7M |
| 48 | Ecoagriculture International INC Oakton | $2.4M | $2.4M | $1.9M |
| 49 | Shenandoah National Park Trust Charlottesvle | $2.3M | $2.2M | $6.6M |
| 50 | Okane Family Foundation INC Great Falls | $2.2M | $0 | $5.4M |