| 1 | Greenacres Foundation Cincinnati | $202.4M | $0 | $388.2M |
| 2 | Western Reserve Land Conservancy Moreland Hls | $29.2M | $29.2M | $34.1M |
| 3 | Cincinnati Nature Center Association Milford | $23.0M | $10.8M | $78.2M |
| 4 | Holden Arboretum Trust Brooklyn | $20.4M | $14.4M | $76.7M |
| 5 | Cardinal Land Conservancy INC Loveland | $14.5M | $12.6M | $20.7M |
| 6 | West Creek Conservancy Parma | $14.0M | $13.9M | $7.8M |
| 7 | Holden Arboretum Kirtland | $13.0M | $11.8M | $198.8M |
| 8 | Institute for Energy Economics and Financial Analysis INC Valley City | $11.5M | $11.5M | $8.1M |
| 9 | Conservancy for Cuyahoga Valley National Park Peninsula | $8.2M | $6.3M | $10.3M |
| 10 | Appalachia Ohio Alliance Columbus | $7.8M | $7.7M | $53.3M |
| 11 | Cleveland Botanical Garden Cleveland | $6.5M | $6.3M | $29.2M |
| 12 | Columbus Region Green Fund Columbus | $5.7M | $5.7M | $8.7M |
| 13 | Joy Outdoor Education Center Foundation INC Clarksville | $5.5M | $3.7M | $9.2M |
| 14 | Great Parks Forever Cincinnati | $5.4M | $5.3M | $24.5M |
| 15 | Black Swamp Conservancy Pemberville | $5.3M | $4.6M | $15.0M |
| 16 | Ohio Environmental Council Columbus | $5.3M | $3.8M | $4.5M |
| 17 | Southern Ohio Diversification Initiative Piketon | $5.2M | $5.2M | $2.9M |
| 18 | Indian Lake Advocacy Group Russells Pt | $5.1M | $5.1M | $5.1M |
| 19 | Friends of the Conservatory Columbus | $4.9M | $4.9M | $11.3M |
| 20 | Hamilton Parks Conservancy Hamilton | $4.9M | $4.4M | $2.3M |
| 21 | Capital Crossroads Spec Improvement District of Columbia INC Columbus | $4.6M | $4.6M | $1.2M |
| 22 | Environmental Health Watch INC Cleveland | $4.3M | $4.0M | $4.0M |
| 23 | Winous Point Marsh Conservancy Port Clinton | $3.6M | $1.3M | $6.1M |
| 24 | Power a Clean Future Ohio Columbus | $3.4M | $3.4M | $3.0M |
| 25 | Little Miami Conservancy Loveland | $3.3M | $3.3M | $11.4M |
| 26 | Athens-hocking County Recycling Centers INC Athens | $3.1M | $3.1M | $2.3M |
| 27 | Horticultural Research Institute Endowment Fund INC Columbus | $3.0M | $3.0M | $23.6M |
| 28 | Cleveland Water Alliance Cleveland | $3.0M | $3.0M | $2.8M |
| 29 | Shaker Lakes Regional Nature Center Cleveland | $2.7M | $2.5M | $7.8M |
| 30 | Wilderness Center INC Wilmot | $2.6M | $2.6M | $18.3M |
| 31 | Glen Helen Association Yellow Spgs | $2.5M | $2.3M | $11.3M |
| 32 | United Plant Savers Rutland | $2.3M | $2.3M | $2.8M |
| 33 | Hba Charitable and Education Foundation Independence | $2.2M | $662K | $1.4M |
| 34 | Keep Cincinnati Beautiful INC Cincinnati | $2.1M | $2.0M | $2.0M |
| 35 | Green Umbrella Cincinnati | $1.8M | $1.8M | $534K |
| 36 | Nopec Foundation INC Solon | $1.5M | $0 | $6.4M |
| 37 | Mill Creek Alliance Reading | $1.4M | $1.4M | $816K |
| 38 | Gorman Heritage Farm Foundation Cincinnati | $1.3M | $1.2M | $2.9M |
| 39 | Cold Creek Foundation Castalia | $1.2M | $1.2M | $1.2M |
| 40 | The Ohio Water Environment Association Columbus | $1.2M | $1.2M | $1.3M |
| 41 | Operator Training Committee of Ohio INC Springfield | $1.1M | $1.1M | $224K |
| 42 | Three Valley Conservation Trust Oxford | $1.1M | $1.1M | $5.8M |
| 43 | Black Environmental Leaders Association Solon | $1.1M | $1.1M | $329K |
| 44 | Greater Ohio Policy Center INC Columbus | $1.1M | $1.1M | $800K |
| 45 | Logan Theater INC Logan | $954K | $954K | $7.2M |
| 46 | Keep Akron Beautiful Akron | $942K | $840K | $847K |
| 47 | Toussaint River Watershed Conservancy Westlake | $932K | $932K | $1.4M |
| 48 | Columbiana County Agricultural Society Lisbon | $900K | $900K | $3.2M |
| 49 | Athens Conservancy Athens | $884K | $879K | $6.5M |
| 50 | Morris Institute Caldwell | $876K | $0 | $6.5M |