| 1 | City of Oaks Foundation INC Raleigh | $105.2M | $105.2M | $109.8M |
| 2 | Serc Reliability Corporation Charlotte | $55.1M | $33.2M | $21.7M |
| 3 | Southern Appalachian Highlands Conservancy Asheville | $30.7M | $30.6M | $148.4M |
| 4 | Triangle Land Conservancy INC Durham | $29.1M | $26.1M | $219.4M |
| 5 | Environmental Research and Raleigh | $15.9M | $9.4M | $18.3M |
| 6 | Grandfather Mountain Stewardship Foundation INC Linville | $15.4M | $10.5M | $38.0M |
| 7 | North Carolina League of Conservation Voters Foundation INC Raleigh | $14.5M | $14.5M | $9.6M |
| 8 | Three Rivers Land Trust INC Salisbury | $14.1M | $12.6M | $39.2M |
| 9 | The Trust for Tomorrow Fairfield | $11.3M | $11.3M | $4.7M |
| 10 | Urban Sustainability Directors Network Sanford | $11.3M | $11.3M | $16.7M |
| 11 | Foothills Conservancy of North Carolina INC Morganton | $10.4M | $9.9M | $45.2M |
| 12 | North Carolina Outward Bound School Asheville | $10.4M | $8.4M | $29.3M |
| 13 | North Carolina Coastal Land Trust Wilmington | $8.3M | $6.3M | $64.8M |
| 14 | Pilot View Resource Conservation & Development Lenoir | $7.6M | $7.6M | $7.5M |
| 15 | Mainspring Conservation Trust INC Franklin | $7.6M | $6.7M | $19.9M |
| 16 | Catawba Lands Conservancy Charlotte | $7.4M | $7.3M | $52.9M |
| 17 | Conservation Trust for North Carolina Raleigh | $7.0M | $3.7M | $12.8M |
| 18 | Wine to Water Boone | $7.0M | $6.7M | $8.4M |
| 19 | Appalachian Voices Boone | $6.8M | $6.8M | $7.9M |
| 20 | Resource Institute INC Winston Salem | $6.7M | $6.7M | $4.4M |
| 21 | Canary Media INC Asheville | $6.6M | $6.6M | $1.4M |
| 22 | Conserving Carolina Hendersonvlle | $6.6M | $6.5M | $45.8M |
| 23 | North Carolina Arboretum Society Asheville | $6.6M | $5.6M | $4.1M |
| 24 | North Carolina Conservation Network Raleigh | $6.3M | $6.3M | $10.7M |
| 25 | Piedmont Land Conservancy Greensboro | $6.1M | $4.5M | $24.2M |
| 26 | Blue Ridge Conservancy Boone | $5.9M | $5.9M | $21.5M |
| 27 | Soil Health Institute Morrisville | $5.8M | $5.8M | $8.3M |
| 28 | Cfa Interpretive Association Pisgah Forest | $5.7M | $4.6M | $3.5M |
| 29 | Soar INC Balsam | $5.5M | $5.5M | $7.3M |
| 30 | Mountaintrue Asheville | $5.2M | $5.1M | $3.4M |
| 31 | Walnut Creek Preserve INC Rutherfordton | $4.6M | $4.6M | $6.2M |
| 32 | Blue Ridge Parkway Foundation Asheville | $4.5M | $4.4M | $7.6M |
| 33 | Blue Ridge Resource Conservation and Development Council Spruce Pine | $4.1M | $4.1M | $3.2M |
| 34 | North Carolina Sustainable Energy Association Raleigh | $3.9M | $3.7M | $2.8M |
| 35 | Water Well Trust Incorporated Davidson | $3.9M | $3.8M | $5.3M |
| 36 | Cape Fear Botanical Garden Fayetteville | $3.9M | $3.9M | $11.4M |
| 37 | Dogwood Alliance INC Asheville | $3.9M | $3.9M | $5.9M |
| 38 | Elliotsville Foundation INC Durham | $3.7M | $0 | $24.9M |
| 39 | Hights INC Cullowhee | $3.6M | $3.6M | $2.0M |
| 40 | Eagles Nest Foundation Incorporated Pisgah Forest | $3.5M | $3.4M | $6.9M |
| 41 | Bald Head Island Conservancy INC Bald Head Isl | $3.5M | $2.2M | $8.7M |
| 42 | Carolina Horse Park Foundation Raeford | $3.5M | $3.1M | $4.5M |
| 43 | The American Chestnut Foundation Asheville | $3.4M | $3.4M | $9.9M |
| 44 | North Carolina Wildlife Federation Raleigh | $3.3M | $3.3M | $8.7M |
| 45 | Southern Highlands Reserve INC Lake Toxaway | $3.1M | $0 | $6.0M |
| 46 | Appalachian Sustainable Agriculture Project Asheville | $2.9M | $2.8M | $4.1M |
| 47 | The Scrap Exchange INC Durham | $2.8M | $2.8M | $6.9M |
| 48 | Association for the Preservation of the Eno River Valley INC Durham | $2.7M | $2.6M | $11.8M |
| 49 | Eo Wilson Biodiversity Foundation Durham | $2.6M | $2.6M | $2.7M |
| 50 | North Carolina Clean Energy Fund Raleigh | $2.4M | $2.4M | $2.8M |