| 1 | University of Louisville Research Foundation INC Louisville | $652.9M | $651.0M | $966.6M |
| 2 | University of Kentucky Research Foundation Lexington | $493.2M | $486.7M | $265.5M |
| 3 | Kentucky Medical Services Foundation INC Lexington | $386.2M | $386.2M | $224.7M |
| 4 | Berea College Berea | $306.1M | $139.3M | $2.1B |
| 5 | University of the Cumberlands INC Williamsburg | $239.6M | $239.6M | $644.9M |
| 6 | Ray and Kay Eckstein Charitable Trust Paducah | $189.3M | $0 | $457.4M |
| 7 | Asbury Theological Seminary Wilmore | $180.4M | $45.3M | $363.8M |
| 8 | Bellarmine University Louisville | $155.6M | $147.9M | $201.1M |
| 9 | Centre College of Kentucky Danville | $152.8M | $0 | $672.8M |
| 10 | Campbellsville University INC Campbellsvlle | $148.9M | $144.4M | $214.8M |
| 11 | Lindsey Wilson University INC Columbia | $98.6M | $83.9M | $134.3M |
| 12 | College Heights Foundation INC Bowling Green | $95.5M | $48.2M | $304.5M |
| 13 | Georgetown College Georgetown | $95.4M | $91.9M | $76.5M |
| 14 | National Association of State Procurement Officials Lexington | $87.5M | $44.0M | $178.1M |
| 15 | University of Pikeville Pikeville | $86.1M | $86.0M | $199.8M |
| 16 | Asbury College Wilmore | $84.1M | $66.5M | $184.4M |
| 17 | Spalding University Louisville | $75.6M | $47.1M | $85.5M |
| 18 | University of Louisville Foundation INC Louisville | $70.0M | $65.9M | $1.2B |
| 19 | Community Action Council for Lex- Fayette Bourb Harr Nich Counties Lexington | $70.0M | $58.2M | $42.6M |
| 20 | Thomas More University INC Crestview Hls | $68.0M | $57.6M | $109.0M |
| 21 | Caney Creek Community Center Pippa Passes | $53.3M | $44.2M | $192.5M |
| 22 | Union Commonwealth University Barbourville | $50.4M | $34.4M | $71.7M |
| 23 | Frontier Nursing University INC Versailles | $50.1M | $50.1M | $137.8M |
| 24 | Lexington School INC Lexington | $47.8M | $25.4M | $84.2M |
| 25 | Kentucky Wesleyan College Owensboro | $47.0M | $33.7M | $66.2M |
| 26 | Partners for Rural Impact INC Berea | $46.1M | $46.1M | $16.4M |
| 27 | Midway University INC Midway | $38.1M | $38.1M | $81.6M |
| 28 | Northern Kentucky University Foundation INC Highland Heights | $37.2M | $10.3M | $164.2M |
| 29 | Christian Academy of Louisville INC Louisville | $36.6M | $32.7M | $34.9M |
| 30 | Louisville Collegiate School Louisville | $36.3M | $36.3M | $60.7M |
| 31 | Wku Student Life Foundation INC Bowling Green | $31.5M | $31.5M | $177.0M |
| 32 | Kentucky Country Day School INC Louisville | $30.1M | $21.7M | $66.0M |
| 33 | Central Kentucky Community Foundation INC Elizabethtown | $27.7M | $4.7M | $41.6M |
| 34 | Brescia University INC Owensboro | $26.3M | $20.8M | $40.9M |
| 35 | Kceoc Community Action Partnership INC Barbourville | $25.6M | $25.6M | $17.4M |
| 36 | Lexington Public Library Lexington | $24.7M | $24.7M | $68.8M |
| 37 | Kentucky Christian University INC Grayson | $21.5M | $19.5M | $37.9M |
| 38 | Eastern Kentucky Univ Foundation INC Giltner Crt 007 38 6206718 Richmond | $21.0M | $7.2M | $117.0M |
| 39 | Lexington Christian Academy INC Lexington | $20.1M | $19.8M | $43.3M |
| 40 | University of Kentucky Alumni Association INC Lexington | $19.7M | $5.3M | $28.9M |
| 41 | Sayre School Lexington | $18.7M | $17.4M | $34.8M |
| 42 | Northern Kentucky Cooperative for Educational Services Cold Spring | $18.6M | $18.6M | $8.8M |
| 43 | Simmons College of Kentucky INC Louisville | $17.5M | $17.3M | $22.1M |
| 44 | Christian Academy of Indiana INC Louisville | $16.5M | $16.5M | $18.1M |
| 45 | Green River Regional Education Cooperative INC Bowling Green | $14.3M | $14.3M | $8.0M |
| 46 | Murray State University Foundation Murray | $14.0M | $14.0M | $215.1M |
| 47 | Bowling Green Warren County Primary Care Center INC Bowling Green | $13.7M | $13.7M | $15.4M |
| 48 | National Center for Families Learning INC Louisville | $12.6M | $12.2M | $20.6M |
| 49 | Family INC Louisville | $11.6M | $11.3M | $33.1M |
| 50 | Kentucky Cattlemens Foundation INC Lexington | $11.4M | $11.4M | $12.4M |