| 1 | The Trustees of Grinnell College Grinnell | $1.4B | $397.4M | $3.2B |
| 2 | Intermediaryed Mount Vernon | $647.7M | $352.5M | $420.5M |
| 3 | Iowa State University Foundation Ames | $435.2M | $143.2M | $2.0B |
| 4 | State University of Iowa Foundation Iowa City | $435.0M | $202.5M | $2.3B |
| 5 | Drake University Des Moines | $363.3M | $256.3M | $628.3M |
| 6 | University of Dubuque Dubuque | $184.2M | $104.0M | $446.9M |
| 7 | St. Ambrose University Davenport | $159.9M | $121.5M | $410.9M |
| 8 | Coe College Cedar Rapids | $155.6M | $81.1M | $174.7M |
| 9 | Des Moines University Osteopathic Medical Center Des Moines | $151.0M | $108.2M | $522.1M |
| 10 | Wartburg College Waverly | $143.5M | $108.6M | $243.0M |
| 11 | Palmer College Foundation Davenport | $140.9M | $93.6M | $320.1M |
| 12 | University of Northern Iowa Foundation Cedar Falls | $140.3M | $35.6M | $290.6M |
| 13 | Luther College Decorah | $138.1M | $132.2M | $326.7M |
| 14 | Iowa Student Loan Liquidity Corp. Wdm | $115.5M | $115.4M | $1.6B |
| 15 | Cornell College Mount Vernon | $98.5M | $87.7M | $230.2M |
| 16 | Dordt University Incorporated Sioux Center | $95.8M | $95.4M | $231.5M |
| 17 | Simpson College Indianola | $93.6M | $66.7M | $145.5M |
| 18 | St. Lukes-jones Regional Medical Center Anamosa | $83.8M | $65.5M | $97.0M |
| 19 | Grand View University Des Moines | $79.6M | $66.1M | $116.5M |
| 20 | Mount Mercy University Cedar Rapids | $75.0M | $53.1M | $100.2M |
| 21 | Buena Vista University Storm Lake | $60.4M | $60.3M | $249.7M |
| 22 | Maharishi International University Fairfield | $59.6M | $55.3M | $87.3M |
| 23 | North Iowa Area Community College Foundation Mason City | $53.1M | $8.0M | $48.6M |
| 24 | Graceland University Lamoni | $52.5M | $46.8M | $117.5M |
| 25 | Central College Pella | $51.8M | $51.7M | $176.3M |
| 26 | William Penn University Oskaloosa | $51.4M | $51.3M | $85.0M |
| 27 | Clarke University of Dubuque Iowa Dubuque | $51.2M | $50.4M | $73.5M |
| 28 | Upper Iowa University Fayette | $50.0M | $48.4M | $110.7M |
| 29 | P E O Foundation Des Moines | $44.9M | $21.0M | $167.8M |
| 30 | Briar Cliff University Sioux City | $44.3M | $41.0M | $50.6M |
| 31 | University of Iowa Strategic Initiatives Fund Iowa City | $37.2M | $37.2M | $1.2B |
| 32 | Cooper-hansen Foundation Des Moines | $31.8M | $8.4M | $28.4M |
| 33 | Waldorf Lutheran College Foundation INC Forest City | $26.5M | $26.5M | $27.6M |
| 34 | Des Moines Christian School Urbandale | $26.5M | $25.7M | $51.1M |
| 35 | Krell Institute INC Ames | $20.0M | $20.0M | $3.8M |
| 36 | Indian Hills Community College Foundation Ottumwa | $19.5M | $2.5M | $40.1M |
| 37 | Iowa Western Community College Foundation Council Bluffs | $19.1M | $5.7M | $38.6M |
| 38 | Resurrection University Ames | $18.8M | $15.4M | $61.1M |
| 39 | Iowa State University Research Park Corp. Ames | $14.2M | $14.0M | $100.4M |
| 40 | Grand View Christian School Association INC Des Moines | $13.1M | $13.0M | $15.6M |
| 41 | Faith Baptist Bible College Ankeny | $12.0M | $11.7M | $21.6M |
| 42 | Kirkwood Community College Foundation Cedar Rapids | $11.9M | $8.5M | $83.0M |
| 43 | Cedar Rapids Public Library Foundation Cedar Rapids | $11.2M | $5.6M | $17.5M |
| 44 | P E O Educational Loan Fund Des Moines | $11.1M | $5.3M | $99.2M |
| 45 | Iowa Association for the Education of Young Children INC Wdm | $11.1M | $11.1M | $3.5M |
| 46 | Iowa High School Athleticassociation Boone | $8.1M | $7.4M | $12.2M |
| 47 | Sioux Center Christian School Sioux Center | $8.0M | $8.0M | $24.7M |
| 48 | The Family Leader Foundation INC Urbandale | $7.7M | $7.0M | $10.5M |
| 49 | Paul B Sharar Foundation for Clinton Campus Clinton | $7.2M | $1.8M | $8.8M |
| 50 | Rsm US Foundation Davenport | $7.2M | $7.0M | $17.5M |