| 1 | Tempus Unlimited INC Stoughton | $2.1B | $2.1B | $223.5M |
| 2 | Sight Savers International INC Boston | $228.1M | $228.1M | $154K |
| 3 | C H M C Anesthesia Foundation INC Boston | $168.5M | $159.8M | $351.1M |
| 4 | Amego INC Attleboro | $106.9M | $106.5M | $99.8M |
| 5 | Joslin Diabetes Center INC Boston | $72.7M | $71.6M | $176.8M |
| 6 | Mab Community Services INC Brookline | $47.9M | $46.5M | $51.6M |
| 7 | Evergreen Center INC Milford | $31.8M | $30.2M | $35.3M |
| 8 | Community Research Initiative of New England INC Boston | $25.5M | $25.5M | $6.0M |
| 9 | Childrens Hospital Pathology Foundation INC Boston | $24.7M | $15.8M | $13.1M |
| 10 | Boston Pediatric Neurosurgical Foundation INC Boston | $21.7M | $20.6M | $25.7M |
| 11 | Integrate Health INC Medway | $14.3M | $14.3M | $18.4M |
| 12 | United Cerebral Palsy Association of Metro Boston INC Watertown | $14.2M | $14.2M | $14.7M |
| 13 | Carroll Center for the Blind INC Newton | $13.7M | $10.8M | $15.5M |
| 14 | Aspire Developmental Services INC Lynn | $12.4M | $12.4M | $8.6M |
| 15 | Ucp of Western Massachusetts INC Pittsfield | $11.9M | $11.8M | $5.6M |
| 16 | Family Reach Foundation Boston | $9.2M | $9.1M | $6.4M |
| 17 | Concussion Legacy Foundation INC Boston | $8.6M | $3.9M | $4.3M |
| 18 | Barton Center for Diabetes Education INC North Oxford | $8.0M | $3.2M | $13.6M |
| 19 | Aids Support Group of Cape Cod INC Provincetown | $7.4M | $7.4M | $2.4M |
| 20 | Sightsavers INC Boston | $7.4M | $7.4M | $1.8M |
| 21 | Association for Autism and Neurodiversity INC Watertown | $6.8M | $6.8M | $2.1M |
| 22 | Rogerson House INC Boston | $6.6M | $6.6M | $9.2M |
| 23 | Doug Flutie Jr Foundation for Autism INC Newton | $6.4M | $3.8M | $4.6M |
| 24 | Joe Andruzzi Foundation INC North Attleboro | $6.1M | $3.7M | $4.0M |
| 25 | Neuroendocrine Tumor Research Foundation Quincy | $5.3M | $4.0M | $21.4M |
| 26 | D-tree International INC Norwell | $5.0M | $5.0M | $3.2M |
| 27 | Massachusetts Health Officers Association Incorporated Walpole | $4.5M | $4.4M | $2.2M |
| 28 | Aids Project Worcester INC Worcester | $4.2M | $4.2M | $3.9M |
| 29 | Lumind Idsc Foundation Woburn | $4.1M | $4.0M | $848K |
| 30 | Multinational Association of Supportive Care in Cancer Newton | $4.0M | $3.1M | $6.5M |
| 31 | 10000 Brains Neuro Ai INC Chestnut Hill | $3.9M | $3.9M | $3.2M |
| 32 | Childrens Oncology Services of Massachusetts INC Brookline | $3.4M | $1.9M | $7.5M |
| 33 | Angiogenesis Foundation INC Cambridge | $3.1M | $3.1M | $2.8M |
| 34 | The Familiescn2a Foundation INC E Longmeadow | $3.1M | $3.1M | $3.6M |
| 35 | Island Autism Group INC West Tisbury | $2.9M | $2.9M | $9.5M |
| 36 | Accelerated Cure Project INC Waltham | $2.9M | $2.9M | $3.5M |
| 37 | Institute for Healthcare Optimization INC Lexington | $2.9M | $2.9M | $1.1M |
| 38 | Alzheimers Family Caregiver Support Center INC Brewster | $2.8M | $1.8M | $6.6M |
| 39 | Pediatric Development Center INC Pittsfield | $2.8M | $2.6M | $3.2M |
| 40 | College Diabetes Network INC Boston | $2.7M | $2.1M | $2.5M |
| 41 | Coalition for Health Ai INC Sudbury | $2.6M | $2.6M | $1.8M |
| 42 | Prostate Health Education Network INC Quincy | $2.5M | $2.5M | $5.4M |
| 43 | Tufts Medical Center Parent INC Boston | $2.3M | $1.3M | $8.6M |
| 44 | Als One INC Whitman | $2.3M | $2.3M | $1.1M |
| 45 | International Society for Infectious Diseases INC Boston | $2.0M | $2.0M | $1.1M |
| 46 | Society for Surgery of the Alimentary Tract Beverly | $2.0M | $1.8M | $5.9M |
| 47 | American Association of Plastic Surgeons Beverly | $1.7M | $1.5M | $8.7M |
| 48 | Impact Melanoma INC Concord | $1.7M | $1.2M | $649K |
| 49 | Retina Society INC Boston | $1.7M | $1.7M | $4.0M |
| 50 | Community Autism Resources Incorporated Swansea | $1.4M | $1.4M | $774K |