| 1 | Saint Louis Symphony Orchestra Saint Louis | $194.7M | $45.2M | $212.4M |
| 2 | Missouri Historical Society Jefferson Memorial Building Saint Louis | $119.2M | $35.1M | $156.3M |
| 3 | St. Louis Art Museum Foundation Saint Louis | $70.6M | $29.7M | $319.2M |
| 4 | Nelson Gallery Foundation Kansas City | $57.6M | $55.6M | $611.5M |
| 5 | Municipal Theatre Association of St. Louis Saint Louis | $49.0M | $37.2M | $127.6M |
| 6 | Kauffman Center for the Performing Arts Kansas City | $43.5M | $40.2M | $439.0M |
| 7 | Union Station Kansas City INC Kansas City | $40.8M | $20.5M | $89.5M |
| 8 | Johnny Morris Wonders of Wildlife Foundation Springfield | $32.6M | $32.4M | $102.3M |
| 9 | American Theatre Guild Kansas City | $32.3M | $32.3M | $39.8M |
| 10 | Starlight Theatre Association of Kansas City INC Kansas City | $28.4M | $23.2M | $50.1M |
| 11 | Kansas City Symphony Kansas City | $26.0M | $22.7M | $89.2M |
| 12 | National World War I Museum and Memorial Kansas City | $25.0M | $24.4M | $58.4M |
| 13 | Regional Cultural & Performing Arts Development Commission St. Louis | $16.8M | $15.0M | $9.2M |
| 14 | Saint Louis Symphony Endowment Trust Saint Louis | $16.0M | -$26.7M | $260.7M |
| 15 | Opera Theatre of Saint Louis Saint Louis | $16.0M | $11.3M | $114.0M |
| 16 | St. Louis Regional Public Media INC Saint Louis | $16.0M | $15.4M | $32.4M |
| 17 | Counterpublic Saint Louis | $15.2M | $15.2M | $14.6M |
| 18 | Magic House Saint Louis | $14.1M | $10.8M | $36.9M |
| 19 | Public Television 19 INC Kansas City | $13.6M | $13.5M | $21.7M |
| 20 | Kansas City Ballet Association Kansas City | $12.0M | $11.0M | $50.2M |
| 21 | Whimsadoodle INC Kansas City | $9.9M | $0 | $9.7M |
| 22 | Jefferson National Parks Association INC Saint Louis | $9.6M | $5.7M | $16.5M |
| 23 | Gateway Creative Broadcasting INC Saint Louis | $9.3M | $8.7M | $20.4M |
| 24 | Center of Creative Arts Saint Louis | $8.8M | $7.9M | $21.5M |
| 25 | Springfield Little Theatre INC Springfield | $8.4M | $8.3M | $13.3M |
| 26 | Thomas H and Diane Demell Jacobsen Ph D Foundation Saint Louis | $8.3M | $0 | $41.9M |
| 27 | The Sheldon Arts Foundation Saint Louis | $8.2M | $6.6M | $20.0M |
| 28 | Lyric Opera of Kansas City INC Kansas City | $7.9M | $7.5M | $31.5M |
| 29 | Unlimited Opportunities INC Boonville | $7.8M | $7.8M | $13.3M |
| 30 | Mid-america Arts Alliance Kansas City | $7.7M | $7.7M | $13.7M |
| 31 | Repertory Theatre of St. Louis Webster Grvs | $7.4M | $5.8M | $2.7M |
| 32 | Negro Leagues Baseball Museum INC Kansas City | $7.3M | $6.8M | $16.7M |
| 33 | National Museum of Toys and Miniatures Kansas City | $7.2M | $2.5M | $44.4M |
| 34 | Kranzberg Arts Foundation Saint Louis | $7.1M | $0 | $23.1M |
| 35 | Kansas City Repertory Theatre INC Kansas City | $7.0M | $6.8M | $17.2M |
| 36 | G & a Outsourcing INC Saint Louis | $6.6M | $932K | $12.1M |
| 37 | Missouri Humanities Council Kansas City | $6.0M | $5.9M | $7.7M |
| 38 | Life Christian Outreach INC Columbia | $5.7M | $5.7M | $3.4M |
| 39 | Hatch Foundation Springfield | $5.6M | $0 | $44.3M |
| 40 | Womenconnect4good INC Springfield | $5.5M | $0 | $2.3M |
| 41 | Cityserve International Springfield | $5.2M | $4.9M | $3.8M |
| 42 | Investigative Reporters and Editors INC Columbia | $4.8M | $3.2M | $8.9M |
| 43 | Contemporary Art Museum St. Louis Saint Louis | $4.7M | $3.6M | $30.7M |
| 44 | The Rabbit Hole Kc N Kansas City | $4.7M | $4.1M | $14.1M |
| 45 | Community Capital Fund Kansas City | $3.9M | $3.9M | $2.9M |
| 46 | The Independent Living Center INC Joplin | $3.8M | $3.8M | $8.2M |
| 47 | Lyric Opera of Kansas City Foundation Kansas City | $3.8M | $797K | $14.3M |
| 48 | World Chess Museum INC Saint Louis | $3.7M | $3.5M | $5.3M |
| 49 | Jazz St. Louis Saint Louis | $3.5M | $3.2M | $6.1M |
| 50 | Missouri Sports Hall of Fame Springfield | $3.4M | $1.2M | $4.3M |