| 1 | Iowa 80 Trucking Museum Walcott | $16.8M | $0 | $44.1M |
| 2 | Anna K Meredith Endowment Trust Des Moines | $14.9M | $3.9M | $56.6M |
| 3 | Edmundson Art Foundation INC Des Moines | $14.6M | $9.4M | $175.7M |
| 4 | National Czech & Slovak Museum & Library Cedar Rapids | $12.9M | $3.5M | $32.8M |
| 5 | Historic Arnolds Park INC Arnolds Park | $11.8M | $11.1M | $50.4M |
| 6 | Living History Farms Foundation Urbandale | $11.2M | $4.1M | $21.6M |
| 7 | Iowa Public Radio INC Des Moines | $11.0M | $10.0M | $16.0M |
| 8 | Earthmoving Legacy Center INC Elkader | $10.1M | $10.1M | $10.2M |
| 9 | Science Center of Iowa Des Moines | $10.0M | $7.1M | $48.5M |
| 10 | Bravo Greater des Moines INC Des Moines | $9.4M | $8.9M | $11.3M |
| 11 | Vesterheim Norwegian American Museum Decorah | $8.1M | $4.7M | $30.7M |
| 12 | Dubuque County Historical Society Dubuque | $7.7M | $7.3M | $38.8M |
| 13 | National Forensic League Wdm | $6.8M | $6.7M | $5.6M |
| 14 | Figge Art Museum Davenport | $6.4M | $4.8M | $14.1M |
| 15 | Des Moines Metro Opera INC Indianola | $6.2M | $6.1M | $6.5M |
| 16 | Hoyt Sherman Place Foundation Des Moines | $6.1M | $6.1M | $59.0M |
| 17 | Community Theatre of Cedar Rapids INC Cedar Rapids | $5.5M | $5.4M | $18.6M |
| 18 | National Sprint Car Hall of Fame & Museum Foundation INC Knoxville | $4.8M | $3.6M | $10.7M |
| 19 | Des Moines Community Playhouse Des Moines | $4.7M | $3.5M | $13.5M |
| 20 | Brucemore INC Cedar Rapids | $4.4M | $3.6M | $20.7M |
| 21 | Greater des Moines Public Art Foundation Des Moines | $4.0M | $4.0M | $4.6M |
| 22 | Des Moines Symphony Association Des Moines | $3.6M | $3.6M | $20.9M |
| 23 | Putnam Museum and Science Center Davenport | $3.2M | $3.1M | $12.0M |
| 24 | Midwest Old Settlers and Threshers Assn INC Mt Pleasant | $3.0M | $3.0M | $6.6M |
| 25 | Quad City Symphony Orchestra Association Davenport | $3.0M | $3.0M | $2.0M |
| 26 | Brenton Arboretum INC Dallas Center | $3.0M | $902K | $6.8M |
| 27 | Danish Immigrant Museum Elk Horn | $3.0M | $2.3M | $11.8M |
| 28 | Ballet Theatre of des Moines Des Moines | $2.9M | $2.8M | $1.3M |
| 29 | Pella Historical Society Pella | $2.8M | $2.6M | $7.4M |
| 30 | Saturday in the Park INC Sioux City | $2.8M | $2.1M | $674K |
| 31 | Englert Civic Theatre INC Non Profit Iowa City | $2.7M | $2.7M | $5.8M |
| 32 | Orpheum Theatre Preservation Project Ltd Sioux City | $2.7M | $2.7M | $8.4M |
| 33 | Sioux City Symphony Orchestra Association Sioux City | $2.7M | $2.1M | $4.9M |
| 34 | Orchestra Iowa INC Cedar Rapids | $2.6M | $2.6M | $7.0M |
| 35 | Ballet Nebraska Council Blfs | $2.5M | $2.5M | $4.2M |
| 36 | Legion-aires Drum and Bugle Corps Dubuque | $2.4M | $2.3M | $1.5M |
| 37 | North Iowa Cultural Center and Museum Clear Lake | $2.4M | $1.3M | $2.2M |
| 38 | Civic Music Association Des Moines | $2.2M | $2.2M | $4.0M |
| 39 | Iowa Childrens Museum Coralville | $2.1M | $2.0M | $3.8M |
| 40 | Preucil School of Music Iowa City | $2.0M | $1.4M | $5.7M |
| 41 | Pearson Lakes Art Center Okoboji | $2.0M | $2.0M | $13.3M |
| 42 | Filmscene Iowa City | $2.0M | $1.9M | $3.5M |
| 43 | Dubuque Museum of Art Dubuque | $1.9M | $1.3M | $6.0M |
| 44 | Grout Museum INC Waterloo | $1.7M | $1.7M | $802K |
| 45 | Mainframe Studios Des Moines | $1.7M | $1.7M | $9.8M |
| 46 | Midwest Honor Flight - Ia & Sd Sioux Center | $1.6M | $1.6M | $1.5M |
| 47 | Downtown Events Group INC Des Moines | $1.5M | $1.5M | $193K |
| 48 | Madison County Historical Society Winterset | $1.5M | $0 | $6.0M |
| 49 | Student Publications Incorporated Iowa City | $1.3M | $1.3M | $2.1M |
| 50 | Four Mounds Foundation Dubuque | $1.3M | $1.3M | $699K |