| 1 | National Wildlife Federation Reston | $159.3M | $156.4M | $178.9M |
| 2 | Trout Unlimited INC Arlington | $114.2M | $109.5M | $60.7M |
| 3 | People for the Ethical Treatment of Animals INC Norfolk | $90.8M | $77.2M | $52.7M |
| 4 | American Bird Conservancy Marshall | $36.4M | $27.0M | $22.6M |
| 5 | Foundation to Support Animal Protection Norfolk | $24.4M | $20.7M | $40.4M |
| 6 | Sea Shepherd Conservation Society Alexandria | $15.2M | $15.1M | $8.4M |
| 7 | Richmond Society for the Prevention of Cruelty to Animals Richmond | $13.8M | $13.4M | $61.0M |
| 8 | Virginia Zoological Society Norfolk | $12.1M | $10.5M | $21.8M |
| 9 | Paws of Honor INC Williamsburg | $11.5M | $10.6M | $2.6M |
| 10 | Colbys Crew Rescue Keswick | $11.4M | $11.4M | $5.7M |
| 11 | Anicira Veterinary Center Harrisonburg | $11.2M | $11.2M | $9.1M |
| 12 | Albemarle Society for the Prevention of Cruelty to Animals Charlottesvle | $9.3M | $4.9M | $13.8M |
| 13 | Richmond Animal League INC N Chesterfld | $8.0M | $5.0M | $8.7M |
| 14 | Cheetah Conservation Fund Alexandria | $7.0M | $6.7M | $3.9M |
| 15 | Virginia Beach SPCA Virginia Bch | $6.4M | $6.1M | $5.6M |
| 16 | Humane Society of Fairfax County INC Fairfax | $6.0M | $2.4M | $17.6M |
| 17 | Lynchburg Humane Society INC Lynchburg | $5.3M | $3.5M | $13.4M |
| 18 | Animal Welfare League of Arlington Va Incorporated Arlington | $5.1M | $4.3M | $10.1M |
| 19 | Animal Welfare League of Alexandria Virginia Incorporated Alexandria | $5.1M | $4.5M | $9.4M |
| 20 | Humane Farm Animal Care Middleburg | $4.9M | $4.9M | $11.4M |
| 21 | Lucky Dog Animal Rescue Arlington | $4.7M | $3.6M | $10.8M |
| 22 | Assisi Animal Clinics of Virginia INC Roanoke | $4.6M | $4.5M | $5.9M |
| 23 | Wildlife Center of Virginia Waynesboro | $4.5M | $4.4M | $6.6M |
| 24 | Middleburg Humane Foundation Marshall | $4.4M | $4.0M | $8.4M |
| 25 | Fauquier S P C a Incorporated Warrenton | $4.2M | $2.5M | $9.5M |
| 26 | Bristol Humane Society Bristol | $3.9M | $3.6M | $3.0M |
| 27 | Mutts with a Mission Virginia Bch | $3.6M | $3.6M | $3.3M |
| 28 | Ompompanoosuc Foundation INC Virginia Beach | $3.2M | $0 | $3.1M |
| 29 | Norfolk Society for the Prevention of Cruelty to Animals INC Norfolk | $2.9M | $2.8M | $1.7M |
| 30 | Rebas Rescue Chesapeake | $2.9M | $2.9M | $665K |
| 31 | Homeward Trails Animal Rescue Incorporated Fairfax Sta | $2.8M | $2.7M | $2.1M |
| 32 | The William and Charlotte Parks Foundation for Animal Welfare Glen Allen | $2.6M | $478K | $5.6M |
| 33 | Institute for Feed Education and Research Arlington | $2.4M | $545K | $3.1M |
| 34 | Rockingham Harrisonburg Society for Prevention of Cruelty to Animals Rockingham | $2.4M | $2.4M | $6.6M |
| 35 | Gloucester-mathews Humane Society Gloucester | $2.3M | $2.2M | $5.9M |
| 36 | Heritage Humane Society Williamsburg | $2.2M | $1.7M | $4.9M |
| 37 | Chesapeake Humane Society INC Chesapeake | $2.1M | $2.1M | $3.0M |
| 38 | Front Range Equine Rescue Great Falls | $2.1M | $2.1M | $11.9M |
| 39 | Society for Integrative and Comparative Biology Herndon | $2.1M | $1.6M | $2.8M |
| 40 | SPCA of Winc & Fred & Clarke C Winchester | $2.0M | $1.3M | $3.4M |
| 41 | Project Perry Louisa | $2.0M | $2.0M | $2.7M |
| 42 | Southside Society for the Prevention of Cruelty to Animals Meherrin | $2.0M | $1.4M | $5.3M |
| 43 | Friends of the Animal Shelter and Guardians of the Homeless Animals Aldie | $1.9M | $1.9M | $2.9M |
| 44 | Pastured Place Charlottesvle | $1.9M | $0 | $170K |
| 45 | Lost Dog Rescue Foundation Falls Church | $1.9M | $1.8M | $4.9M |
| 46 | Wounded Paw Project Clifton | $1.8M | $1.8M | $644K |
| 47 | Augusta Regional SPCA INC Staunton | $1.8M | $1.8M | $5.9M |
| 48 | Roanoke Valley SPCA Roanoke | $1.7M | $1.2M | $5.6M |
| 49 | Peninsula Society for the Preven Tion of Cruelty to Animals INC Newport News | $1.7M | $1.5M | $1.5M |
| 50 | Blue Ridge Zoological Society of Virginia INC Roanoke | $1.6M | $1.6M | $2.0M |