| 1 | Babinski Foundation Sioux Falls | $13.9M | $0 | $66.2M |
| 2 | Sioux Falls Zoo & Aquarium Sioux Falls | $12.8M | $12.8M | $33.3M |
| 3 | Hanson Family Foundation for the Well-being of Gods Creatures Sioux Falls | $3.8M | $0 | $4.7M |
| 4 | Institute of Range & the American Mustang Hot Springs | $3.5M | $2.2M | $6.7M |
| 5 | Sioux Falls Humane Society Sioux Falls | $2.5M | $2.4M | $29.1M |
| 6 | Humane Society of the Black Hills Rapid City | $1.6M | $1.6M | $2.4M |
| 7 | Aberdeen Area Humane Society Aberdeen | $1.5M | $1.5M | $3.0M |
| 8 | Tanka Fund Kyle | $1.1M | $1.1M | $744K |
| 9 | Youth for the Quality Care of Animals INC Pierre | $984K | $984K | $682K |
| 10 | Heartland Humane Society INC Yankton | $923K | $435K | $1.2M |
| 11 | South Dakota Discovery Center Pierre | $777K | $773K | $518K |
| 12 | Battle Mountain Humane Society Hot Springs | $581K | $581K | $560K |
| 13 | Lake Area Zoological Society Watertown | $568K | $567K | $2.1M |
| 14 | Mitchell Rodeo Foundation Mitchell | $498K | $498K | $1.6M |
| 15 | The Brookings Regional Humane Society INC Brookings | $374K | $364K | $407K |
| 16 | Glacial Lakes Humane Society and Shelter Watertown | $290K | $290K | $861K |
| 17 | Beadle County Humane Society Huron | $274K | $274K | $382K |
| 18 | Almost Home Canine Rescue Sioux Falls | $252K | $252K | $27K |
| 19 | Sioux Empire Pit Bull Rescue INC Sioux Falls | $239K | $239K | $373K |
| 20 | Dakota Dachshund Rescue INC Sioux Falls | $227K | $227K | $226K |
| 21 | Black Hills Raptor Center Caputa | $214K | $214K | $643K |
| 22 | Pierre-fort Pierre Area Humane Society INC Pierre | $193K | $193K | $579K |
| 23 | Save Them All Rescue Services Hot Springs | $185K | $74K | $1 |
| 24 | Cats Meow Foster Care INC Mitchell | $181K | $181K | $365K |
| 25 | Saving Huey Foundation Sioux Falls | $178K | $178K | $182K |
| 26 | Lake Traverse Animal Rezcue Sisseton | $166K | $166K | $76K |
| 27 | Shiloh Charitable Trust Deadwood | $145K | $145K | $170K |
| 28 | Sd Caring for Canines Rapid City | $130K | $130K | $2K |
| 29 | South Dakota Wildlife Federation INC Pierre | $128K | $115K | $207K |
| 30 | A Place for Meow Rescue Rapid City | $125K | $125K | $4K |
| 31 | South Dakota Walleyes Unlimited Rapid City | $121K | $39K | $72K |
| 32 | Allcatsrescue Sioux Falls | $107K | $107K | $15K |
| 33 | Gentle Spirit Horses Rescue & Sanctuary Scotland | $102K | $102K | $25K |
| 34 | South Dakota West River Spay-neutercoalition Deadwood | $89K | $89K | $12K |
| 35 | Codington County Pro Phesants INC Watertown | $84K | $30K | $82K |
| 36 | Happy Tails Haven Piedmont | $79K | $79K | $196 |
| 37 | Sioux Empire Tnr Coalition Sioux Falls | $76K | $76K | $20K |
| 38 | Horse Help Providers INC Crooks | $72K | $72K | $32K |
| 39 | Friends of the Twin Cities Animal Shelter Lead | $65K | $65K | $203K |
| 40 | Milbank Animal Rescue Services Milbank | $56K | $56K | $13K |
| 41 | Spirit of the Hills Wildlife Sanctuary a Non Profit Sd Corp. Spearfish | $50K | $50K | $1 |
| 42 | Oglala Pet Project Kyle | $50K | $50K | $244K |
| 43 | Black Hills Mountain Lion Foundation Piedmont | $18K | $4K | $519 |
| 44 | Webster Paws Walk Webster | $7K | $7K | $31K |
| 45 | Warhorse Liberation Rapid City | $5K | $5K | $1 |
| 46 | Little Prairie Collective Vale | $1 | $0 | $1 |
| 47 | E T Farms Animal Rescue & Sanctuary Delmont | $0 | $0 | $0 |
| 48 | Western Bat Working Group Rapid City | $0 | $0 | $0 |
| 49 | Wbfi Research Foundation Britton | $0 | $0 | $0 |
| 50 | Wildlife Habitat and More Viborg | $0 | $0 | $0 |