| 1 | Massachusetts Society for the Prevention of Cruelty to Animals Boston | $176.0M | $144.8M | $252.2M |
| 2 | New England Aquarium Corporation Boston | $55.0M | $54.6M | $126.6M |
| 3 | International Fund for Animal Welfare INC Yarmouth Port | $53.8M | $36.5M | $53.1M |
| 4 | Animal Rescue League of Boston Dedham | $49.4M | $21.9M | $141.0M |
| 5 | Commonwealth Zoological Corporation Boston | $31.8M | $29.1M | $58.9M |
| 6 | New England Wildlife Center S Weymouth | $30.8M | $30.8M | $38.6M |
| 7 | Norcross Wildlife Foundation INC Wales | $10.7M | $0 | $59.7M |
| 8 | Ecohealth Alliance INC Boston | $9.5M | $8.0M | $1.7M |
| 9 | Second Chance Animal Services INC E Brookfield | $8.4M | $8.2M | $7.1M |
| 10 | Preservation of At-risk Wildlife Paw Foundation Boston | $8.4M | $0 | $6.2M |
| 11 | Buttonwood Park Zoological Society INC New Bedford | $7.7M | $1.7M | $2.5M |
| 12 | Baypath Humane Society of Hopkinton INC Hopkinton | $6.8M | $2.4M | $6.4M |
| 13 | Dakin Pioneer Valley Humane Societyinc Springfield | $6.2M | $5.5M | $7.3M |
| 14 | American Fondouk Maintenance Committee INC Boston | $5.7M | $1.8M | $19.9M |
| 15 | New England Regional Fishery Management Council Newburyport | $5.3M | $5.3M | $1.3M |
| 16 | Nantucket Safe Harbor for Animals INC Nantucket | $5.2M | $4.2M | $9.0M |
| 17 | Yankee Golden Retriever Rescue INC Hudson | $4.5M | $646K | $5.6M |
| 18 | Berkshire Humane Society INC Pittsfield | $3.4M | $2.8M | $6.7M |
| 19 | Foster Parrots Ltd Rockland | $3.3M | $2.8M | $7.2M |
| 20 | Cape Ann Animal Aid Association INC Gloucester | $3.0M | $2.6M | $7.9M |
| 21 | Gorilla Rehabilitation and Conservation Education Grace Cente Falmouth | $2.8M | $2.8M | $4.0M |
| 22 | Four Paws International INC Boston | $2.5M | $2.5M | $407K |
| 23 | Buddy Dog Humane Society INC Sudbury | $2.5M | $1.4M | $11.9M |
| 24 | Amboseli Trust for Elephants Newbury | $1.9M | $1.9M | $1.7M |
| 25 | Vested Interest in K9s INC East Taunton | $1.8M | $1.8M | $4.6M |
| 26 | Animal Shelter INC Sterling | $1.6M | $1.6M | $2.0M |
| 27 | Sweet Paws Rescue Groveland | $1.4M | $1.4M | $2.9M |
| 28 | International Animal Rescue US Berlin | $1.4M | $1.4M | $492K |
| 29 | Ellen M Gifford Sheltering Home Corporation Brighton | $1.4M | $1.1M | $4.6M |
| 30 | Harmony Fund INC Holden | $1.4M | $1.4M | $56K |
| 31 | Quincy Animal Shelter INC Quincy | $1.4M | $719K | $2.0M |
| 32 | New England Anti-vivisection Society Boston | $1.4M | $1.4M | $5.0M |
| 33 | Merwin Memorial Free Clinic for Animals INC Allston | $1.3M | $450K | $5.6M |
| 34 | Forest Park Zoological Society INC Springfield | $1.3M | $1.3M | $637K |
| 35 | Merrimack River Feline Rescue Society INC Salisbury | $1.3M | $1.2M | $6.8M |
| 36 | Worcester Animal Rescue League Worcester | $1.2M | $1.2M | $5.6M |
| 37 | Standish Humane Society INC Duxbury | $1.2M | $647K | $2.4M |
| 38 | Birdscaribbean Natick | $1.1M | $984K | $842K |
| 39 | Grey2k USA Worldwide INC Arlington | $1.1M | $739K | $1.4M |
| 40 | Humane Society-lowell Lowell | $1.0M | $1.0M | $694K |
| 41 | Last Hope K9 Rescue Boston | $1.0M | $888K | $288K |
| 42 | Humane Society & Shelter Southcoast N Dartmouth | $1.0M | $790K | $1.9M |
| 43 | John Butler Mulliken Foundation INC Waltham | $991K | $0 | $1.3M |
| 44 | Wild Care INC Eastham | $985K | $867K | $1.8M |
| 45 | Beneath the Waves INC Charlestown | $974K | $974K | $644K |
| 46 | Pet Partners INC Fall River | $968K | $847K | $302K |
| 47 | Gunung Palung Orangutan Conservation Program INC Lexington | $922K | $922K | $263K |
| 48 | Wellfleet Shellfish Promotion and Tasting INC Wellfleet | $883K | $883K | $1.1M |
| 49 | World Federation for Animals INC Jamaica Plain | $823K | $823K | $691K |
| 50 | Communities Living Among Wildlife Sustainably Conservancy INC Worcester | $821K | $809K | $660K |