| 1 | Animal Rescue League of Iowa Incorporated Des Moines | $13.2M | $13.0M | $13.7M |
| 2 | Blank Park Zoo Foundation INC Des Moines | $12.5M | $11.9M | $28.5M |
| 3 | Great Outdoors Foundation Wdm | $5.3M | $5.2M | $12.1M |
| 4 | Humane Society of Scott County Davenport | $3.3M | $2.8M | $4.6M |
| 5 | Humane Society of North Iowa Mason City | $2.5M | $1.3M | $4.8M |
| 6 | Protectors of Animal Welfare of North Lee County Fort Madison | $2.5M | $2.5M | $6.7M |
| 7 | Cedar Valley Humane Society Cedar Rapids | $2.4M | $2.3M | $8.4M |
| 8 | Monarch Research Project Marion | $1.9M | $1.3M | $1.8M |
| 9 | Siouxland Humane Society INC Sioux City | $1.8M | $1.3M | $5.7M |
| 10 | Dubuque Humane Society Dubuque | $1.6M | $1.5M | $4.1M |
| 11 | Midlands Humane Society Council Blfs | $1.4M | $1.3M | $6.6M |
| 12 | Cedar Bend Humane Society INC Waterloo | $1.4M | $1.4M | $3.5M |
| 13 | Happy Doggo Ames | $1.1M | $1.1M | $874K |
| 14 | A Heinz57 Pet Rescue and Transport INC De Soto | $909K | $904K | $1.8M |
| 15 | It Takes a Village Animal Rescue & Resources Muscatine | $847K | $625K | $14K |
| 16 | Ny Foundation Glenwood | $792K | $0 | $725K |
| 17 | Blank Park Endowment Corporation Des Moines | $717K | $422K | $9.8M |
| 18 | Kiya Koda Humane Society Indianola | $716K | $715K | $872K |
| 19 | Animal Lifeline of Iowa INC Des Moines | $705K | $678K | $1.8M |
| 20 | Friends of the Animal Center Foundation Iowa City | $692K | $678K | $1.9M |
| 21 | Animal Rescue League of Marshalltown Iowa Marshalltown | $655K | $620K | $1.9M |
| 22 | Iowa Humane Alliance Cedar Rapids | $631K | $631K | $1.4M |
| 23 | Iowa Farm Sanctuary Oxford | $630K | $630K | $543K |
| 24 | K9sensus Foundation Lucas | $609K | $609K | $122K |
| 25 | Muscatine Humane Society INC Muscatine | $598K | $598K | $1.8M |
| 26 | Animal Welfare Friends Monticello | $594K | $590K | $677K |
| 27 | Clinton Humane Society Clinton | $582K | $571K | $2.3M |
| 28 | Des Moines County Humane Society Burlington | $551K | $425K | $1.9M |
| 29 | Humane Society of North Central Iowa Fort Dodge | $539K | $490K | $1.3M |
| 30 | Giving Back Outdoors Wdm | $536K | $536K | $186K |
| 31 | Furry Friends Refuge Wdm | $524K | $524K | $2.4M |
| 32 | Raptor Resource Project Decorah | $502K | $464K | $344K |
| 33 | Muddy Paws Second Chance Rescue Council Blfs | $449K | $449K | $157K |
| 34 | Noahs Hope Sioux City | $439K | $439K | $827K |
| 35 | Wildlife Lodge & Clinic Sioux City | $418K | $25K | $665K |
| 36 | Bailing Out Benji Ames | $409K | $363K | $516K |
| 37 | Humane Society of Northwest Iowa Milford | $394K | $394K | $319K |
| 38 | Last Hope Animal Rescue INC Cedar Rapids | $392K | $392K | $401K |
| 39 | Muscatine Humane Society INC Endowment Trust Muscatine | $380K | $123K | $2.3M |
| 40 | Ape Cognition & Communication Institute Des Moines | $374K | $355K | $10.9M |
| 41 | Heartland Humane Society Ltd Ottumwa | $367K | $359K | $711K |
| 42 | Boone Area Humane Society INC Boone | $361K | $361K | $456K |
| 43 | Critter Crusaders of Cedar Rapids INC Cedar Rapids | $347K | $334K | $50K |
| 44 | Marion County Humane Society Knoxville | $345K | $345K | $340K |
| 45 | People for Pets Spencer | $343K | $331K | $542K |
| 46 | Humane Society of Northeast Iowa Decorah | $334K | $334K | $837K |
| 47 | Iowa Wildlife Center Ames | $324K | $324K | $684K |
| 48 | Stephen Memorial Animal Shelter INC Oskaloosa | $322K | $322K | $24K |
| 49 | Safe Haven of Iowa County Williamsburg | $299K | $299K | $475K |
| 50 | Emmet County Animal Shelter Estherville | $283K | $283K | $342K |