| 1 | Lowry Park Zoological Society of Tampa INC Tampa | $57.7M | $48.6M | $83.5M |
| 2 | Fish & Wildlife Foundation of Florida INC Tallahassee | $53.4M | $30.0M | $90.6M |
| 3 | Florida Aquarium INC Tampa | $48.5M | $40.6M | $60.6M |
| 4 | Jacksonville Zoological Society INC Jacksonville | $43.3M | $39.3M | $103.8M |
| 5 | Peggy Adams Animal Rescue League of the Palm Beaches INC West Palm Bch | $31.4M | $15.8M | $145.2M |
| 6 | East Coast Zoological Society of Florida INC Melbourne | $31.4M | $30.8M | $70.2M |
| 7 | Ocean Habitat and Wildlife Rescue INC West Palm Bch | $30.2M | $30.2M | $49.5M |
| 8 | Florida Veterinary Medicine Faculty Association INC Gainesville | $28.2M | $28.2M | $17.3M |
| 9 | Clearwater Marine Aquarium INC Clearwater | $24.8M | $22.9M | $102.2M |
| 10 | K9s for Warriors INC Ponte Vedra | $24.7M | $24.2M | $38.5M |
| 11 | Humane Society of Tampa Bay INC Tampa | $24.5M | $17.7M | $30.6M |
| 12 | Zoological Society of the Palm Beaches INC West Palm Bch | $23.4M | $22.3M | $55.9M |
| 13 | Big Dog Ranch Rescue INC Lxhtchee Grvs | $20.6M | $18.6M | $22.9M |
| 14 | Igfa Foundation INC Dania Beach | $16.4M | $3.2M | $22.4M |
| 15 | Naples Zoo INC Naples | $13.1M | $12.1M | $42.8M |
| 16 | Zoo Miami Foundation INC Miami | $12.9M | $7.9M | $36.4M |
| 17 | Society for the Prevention of Cruel Ty to Animals Tampa Bay Florida in Largo | $12.6M | $8.6M | $10.9M |
| 18 | Humane Society of Broward County Ft Lauderdale | $12.6M | $11.8M | $25.5M |
| 19 | National Save the Sea Turtle Foundation INC N Palm Beach | $11.5M | $11.5M | $38.6M |
| 20 | Wags Pet Clinic INC Tampa | $11.5M | $10.6M | $6.3M |
| 21 | S P C a INC Lakeland | $10.8M | $8.4M | $12.9M |
| 22 | First Coast No More Homeless Pets INC Jacksonville | $10.6M | $10.6M | $6.7M |
| 23 | Furry Friends Humane INC Jupiter | $10.1M | $8.8M | $14.6M |
| 24 | Humane Society of the Treasure Coast INC Palm City | $9.7M | $4.1M | $12.2M |
| 25 | Jacksonville Humane Society Jacksonville | $9.3M | $8.1M | $35.2M |
| 26 | Central Florida Zoological Society INC Lake Monroe | $9.0M | $9.0M | $17.0M |
| 27 | Alaqua Animal Refuge INC Freeport | $8.8M | $7.8M | $13.9M |
| 28 | Humane Society of Collier County INC Naples | $8.2M | $5.7M | $15.0M |
| 29 | Pet Alliance of Greater Orlando INC Orlando | $8.1M | $7.8M | $26.9M |
| 30 | Gulf Coast Humane Society INC Fort Myers | $7.8M | $7.0M | $14.6M |
| 31 | Center for Orangutan and Chimpanzee Conservation INC Wauchula | $7.3M | $7.3M | $18.9M |
| 32 | Halifax Humane Society INC Daytona Beach | $7.2M | $4.9M | $18.9M |
| 33 | Paws 2 Help INC West Palm Bch | $7.1M | $7.1M | $2.4M |
| 34 | Dolphin Research Center INC Grassy Key | $6.8M | $6.3M | $10.3M |
| 35 | Humane Society of Sarasota County INC Sarasota | $6.8M | $6.0M | $24.4M |
| 36 | Tri County Humane Society Boca Raton | $6.7M | $6.6M | $28.5M |
| 37 | Flamingo Gardens INC Davie | $6.6M | $6.6M | $6.5M |
| 38 | Humane Society of Pinellas INC Clearwater | $6.6M | $3.5M | $21.7M |
| 39 | Save the Chimps INC Fort Pierce | $6.5M | $5.3M | $19.0M |
| 40 | Orcat INC Key Largo | $6.2M | $5.8M | $7.4M |
| 41 | Bonefish & Tarpon Trust INC Miami | $6.0M | $5.5M | $2.5M |
| 42 | Hidden Harbor Marine Environmental Project INC Marathon | $5.9M | $5.4M | $14.6M |
| 43 | Veterinary Care Foundation Incorporated Reddick | $5.8M | $5.8M | $4.3M |
| 44 | Mark and Carol Zemmel Fund for the Human Animal Connection INC Coral Gables | $5.8M | $0 | $7.4M |
| 45 | Marion and Richard Graham Foundation Ft Lauderdale | $5.6M | $0 | $36.6M |
| 46 | Ding Darling Wildlife Society INC Sanibel | $5.6M | $5.1M | $27.4M |
| 47 | Florida Aid to Animals Spay Neuter Medical Facility INC Melbourne | $5.6M | $5.6M | $1.9M |
| 48 | Suncoast Humane Society INC Englewood | $5.2M | $4.1M | $17.3M |
| 49 | Humane Society of Vero Beach Fla Vero Beach | $5.1M | $4.2M | $6.7M |
| 50 | Great Plains Conservation Foundation Jupiter | $5.1M | $5.0M | $7.7M |